Team:Paris Saclay/Labwork
From 2013.igem.org
(Difference between revisions)
Line 53: | Line 53: | ||
* Colony PCR of Bba_K1155001 in PSB1C3 in DH5α | * Colony PCR of Bba_K1155001 in PSB1C3 in DH5α | ||
* Electrophoresis of Colony PCR products : Bba_K1155001 in PSB1C3 [[File:Psright.png|20px]] | * Electrophoresis of Colony PCR products : Bba_K1155001 in PSB1C3 [[File:Psright.png|20px]] | ||
+ | |||
+ | 07/12/13 | ||
+ | * Stock of clones 5, 6 for Bba_K1155001 | ||
+ | * Extraction of clones 5, 6 for Bba_K1155001 from DH5α | ||
Line 68: | Line 72: | ||
07/11/13 | 07/11/13 | ||
* Colony PCR of Bba_K1155002 in PSB1C3 in DH5α | * Colony PCR of Bba_K1155002 in PSB1C3 in DH5α | ||
- | *Electrophoresis of Colony PCR products : Bba_K1155002 in PSB1C3 [[File:Psright.png|20px]] | + | * Electrophoresis of Colony PCR products : Bba_K1155002 in PSB1C3 [[File:Psright.png|20px]] |
+ | |||
+ | 07/12/13 | ||
+ | * Stock of clones 5, 6, 7, 8 for Bba_K1155002 | ||
+ | * Streak of Bba_K1155002 | ||
+ | Extraction of clones 5, 6, 7, 8 for Bba_K1155002 from DH5α | ||
+ | |||
Line 85: | Line 95: | ||
* Digestion of Bba_K592009 by NotI, XhoI, EcoRI, EcoRI/PstI | * Digestion of Bba_K592009 by NotI, XhoI, EcoRI, EcoRI/PstI | ||
* Electrophoresis of the digestion of Bba_K592009 by NotI, XhoI, EcoRI, EcoRI/PstI [[File:Psright.png|20px]] | * Electrophoresis of the digestion of Bba_K592009 by NotI, XhoI, EcoRI, EcoRI/PstI [[File:Psright.png|20px]] | ||
+ | |||
+ | 07/12/13 | ||
+ | * Transformation of Bba_B0015, Bba_B0017 [[File:Psright.png|20px]] | ||
+ | * Design of RBS-AmilCP oligos | ||
Line 113: | Line 127: | ||
07/10/13 | 07/10/13 | ||
* Transformation of Bba_I732019 in DH5α [[File:Pswrong.png|20px]] | * Transformation of Bba_I732019 in DH5α [[File:Pswrong.png|20px]] | ||
+ | |||
+ | 07/12/13 | ||
+ | * Transformation of Bba_I732019, Bba_B0015, Bba_B0017 [[File:Psright.png|20px]] | ||
Line 136: | Line 153: | ||
* Electrophoresis of Colony PCR products : BphR2 in PSB1C3 [[File:Psright.png|20px]] | * Electrophoresis of Colony PCR products : BphR2 in PSB1C3 [[File:Psright.png|20px]] | ||
+ | 07/12/13 | ||
+ | * Stock of clones 3, 4 for BphR2 | ||
+ | * Streak of BphR2 | ||
+ | * Extraction of clones 3, 4 for BphR2 from DH5α | ||
=='''PSB3K3'''== | =='''PSB3K3'''== |
Revision as of 15:48, 26 September 2013
Contents |
Labwork overview
Bba_K1155000
07/01/13
- Digestion of PSB1C3 plasmid and PCR products : Pfnr by EcoRI/ PstI
- Ligation of PSB1C3 and Pfnr
07/02/13
07/03/13
- Colony PCR of Bba_K1155000 to check good insertion of Pfnr in PSB1C3
- New culture of Bba_K1155000
07/04/13
- Estimation of Pfnr Colony PCR size fragments thanks to Gene manager
- Electrophoresis of Colony PCR products
- Stock of Bba_K1155000
- Culture of Bba_K1155000
- Extraction of Bba_K1155000 from DH5α
- Digestion of Bba_K1155000 by NotI, MluI, HpaI to check good insertion of Pfnr in PSB1C3 again
07/05/13
- Electrophoresis of the digestion of Bba_K1155000 by NotI, HpaI, MluI
- Extraction of Bba_K1155000 from DH5α
Bba_K1155001
07/09/13
- PCR purification of BphR1
- Digestion PCR products : BphR1 and digestion of PSB1C3
- Denaturation of EcoRI/PstI used for the digestion of PCR products : BphR1 and PSB1C3
- Ligation of BphR1 and PSB1C3
07/10/13
07/11/13
- Colony PCR of Bba_K1155001 in PSB1C3 in DH5α
- Electrophoresis of Colony PCR products : Bba_K1155001 in PSB1C3
07/12/13
- Stock of clones 5, 6 for Bba_K1155001
- Extraction of clones 5, 6 for Bba_K1155001 from DH5α
Bba_K1155002
07/09/13
- PCR purification of BphA1
- Digestion PCR products : BphA1 and digestion of PSB1C3
- Denaturation of EcoRI/PstI used for the digestion of PCR products : BphA1 and PSB1C3
- Ligation of BphA1 and PSB1C3
07/10/13
07/11/13
- Colony PCR of Bba_K1155002 in PSB1C3 in DH5α
- Electrophoresis of Colony PCR products : Bba_K1155002 in PSB1C3
07/12/13
- Stock of clones 5, 6, 7, 8 for Bba_K1155002
- Streak of Bba_K1155002
Extraction of clones 5, 6, 7, 8 for Bba_K1155002 from DH5α
Bba_K1155003
07/02/13
07/03/13
- New culture of Bba_K592009
07/05/13
- Extraction of Bba_K592009 from DH5α
07/08/13
- Digestion of Bba_K592009 by NotI, XhoI, EcoRI, EcoRI/PstI
- Electrophoresis of the digestion of Bba_K592009 by NotI, XhoI, EcoRI, EcoRI/PstI
07/12/13
Bba_K1155004
Bba_K1155005
Bba_K1155006
Bba_K1155007
07/02/13
07/03/13
- New culture of Bba_I732017
07/05/13
- Extraction of Bba_I732017 from DH5α
07/08/13
- Digestion of Bba_I732017 by NotI, XhoI, EcoRI, EcoRI/PstI
- Electrophoresis of the digestion of Bba_I732017 by NotI, XhoI, EcoRI, EcoRI/PstI
07/10/13
07/12/13
Bba_K1155008
Bba_K1155009
07/01/13
- Design of oligos for amplification of BphR2 gene of Pseudomonas pseudoalcaligenes, Pseudomonas oleovorans
07/09/13
- PCR purification of BphR2
- Digestion PCR products : BphR2 and digestion of PSB1C3
- Denaturation of EcoRI/PstI used for the digestion of PCR products : BphR2 and PSB1C3
- Ligation of BphR2 and PSB1C3
07/10/13
07/11/13
07/12/13
- Stock of clones 3, 4 for BphR2
- Streak of BphR2
- Extraction of clones 3, 4 for BphR2 from DH5α
PSB3K3
07/09/13
07/10/13
07/11/13
- Extraction of Bba_J04450 from DH5α
- Digestion of Bba_J04450 to chek the size for the plasmid
- Electrophoresis of the digestion of Bba_J04450