Team:NTU Taiwan/index.html

                             <li><a href="#teamCalendar"> Team_Calendar </a> </li>

                 White and brown adipose tissues (WAT and BAT) serve important opposite functions in overall energy balance. While WAT is specialized in energy storage in the form of triacylglycerols, BAT functions to dissipate energy in the form of heat (thermogenesis).BAT possess abundant mitochondria with uncoupling proteins 1(UCP1). UCP1 is a 6-transmembrane protein in inner mitochondria membrane, and it has been proposed to constitute three symmetrical membrane-spanning &ldquo;Us&rdquo;, each comprising about 100 amino acids(fig.?).The functional UCP is believed to be dimer.

             <img class="tipReveal img-responsive" src="images/image001.png">

                 (fig.? The protein (33kDa,306 amino acids) contains six transmembrane alpha-helices, proposed to constitute three symmetric repeats.)

             UCP has been found in several species, including mammals and plants. Skunk cabbage(Symplocarpus foetidus)is a thermogenic plant, using srUCPs to resist in dramatic temperature decreases in the environment. In 1999, Dr. Kikukatsu Ito found that there are two types of SrUCPs, designated as SrUCPA and SrUCPB [4]. SrUCPA is proposed to be the functional thermogenic prtein, and SrUCPB serves as a regulator in thermogenesis in Skunk cabbage.[5][6] We use SrUCPA gene as our material to construct our plasmid.

             [1] Brown adipose tissue: function and physiological significance. Cannon B, Nedergaard J. Physiol Rev. 2004 Jan.

             [2] Uncoupling proteins: the issues from a biochemist point of view. Klingenberg M, Echtay KS. Biochim Biophys Acta. 2001 Mar 1;1504(1):128-43.

             [3] Mitochondrial Uncoupling Proteins in Mammals and Plants. Jirı´ Borecky´, Ivan G. Maia, and Paulo Arruda. Bioscience Reports, Vol. 21, No. 2, April 2001 (2001).

             [4] Isolation of two distinct cold-inducible cDNAs encoding plant uncoupling proteins from the spadix of skunk cabbage (Symplocarpus foetidus). Kikukatsu Ito. Plant Science Vol. 149, Issue 2, 3 Dec. 1999, P.167–173.

             [5] Characterization of the plant uncoupling protein, SrUCPA, expressed in spadix mitochondria of the thermogenic skunk cabbage. Yasuko Ito-Inaba, Yamato Hida, Megumi Ichikawa, Yoshiaki Kato and Tetsuro Yamashita. Journal of Experimental Botany, Vol. 59, No. 4, pp. 995–1005, 2008.

             [6] Functional Coexpression of the Mitochondrial Alternative Oxidase and Uncoupling Protein Underlies Thermoregulation in the Thermogenic Florets of Skunk Cabbage. Yoshihiko Onda, Yoshiaki Kato, Yukie Abe, Takanori Ito, Miyuki Morohashi, Yuka Ito,Megumi Ichikawa4, Kazushige Matsukawa, Yusuke Kakizaki, Hiroyuki Koiwa, and Kikukatsu Ito. Plant Physiology, February 2008, Vol. 146, pp. 636–645.

                     [*]Thermoregulation: what role for UCPs in mammals and birds?Mozo J, Emre Y, Bouillaud F, Ricquier D, Criscuolo F. Biosci Rep. 2005 Jun-Aug;25(3-4):227-49.

             <img class="pull-right img-responsive" src="./images/UT_conference.jpg">

             <img class="pull-left img-responsive" src="./images/UT_conference in Tokyo.jpg">

             <img class="pull-right img-responsive" src="./images/Purdue_conference.jpg">

             <img class="pull-left img-responsive" src="./images/Berkeley_conference.jpg" width=700 height=525>

             <img class="pull-right img-responsive" src="./images/Chiao_Tung_Conference/(27).jpg" width=700>

                     <img class="pull-left img-responsive" src="./images/AZALEA/(1).jpg">

                     <img class="pull-right img-responsive" src="./images/AZALEA/(10).jpg">

        <h1 class="header">Poster Presentation</h1>

                     <img class="img-responsive" src="./images/MEETING/Meeting-(10).jpg" width=400>

                             <img class="img-responsive" src="./images/Human_practice/HUMANPRACTICE_2.jpg" style="margin-right: 30px">

     <script type="text/template" id="teamCalendar">

                     <div class="col-md-5"><img class="img-responsive" src="./images/people/李易遠.jpg"></div>

                     <div class="col-md-4"><img class="img-responsive" src="./images/people/李易遠.jpg"></div>

                     <div class="col-md-4"><img class="img-responsive" src="./images/people/吳泰億.jpg"></div>

                     <div class="col-md-4"><img class="img-responsive" src="./images/people/呂宗諭.jpg"></div>

                     <div class="col-md-4"><img class="img-responsive" src="./images/people/張宏宇.jpg"></div>

                     <div class="col-md-4"><img class="img-responsive" src="./images/people/王德緯.jpg"></div>

                     <div class="col-md-4"><img class="img-responsive" src="./images/people/馬蓁華.jpg"></div>

                     <div class="col-md-4"><img class="img-responsive" src="./images/people/陳俋秀.jpg"></div>

                     <div class="col-md-4"><img class="img-responsive" src="./images/people/盧彥云.jpg"></div>

                     <div class="col-md-4"><img class="img-responsive" src="./images/people/王柏軒.jpg"></div>

                     <div class="col-md-4"><img class="img-responsive" src="./images/people/李威.jpg"></div>

                     <div class="col-md-4"><img class="img-responsive" src="./images/people/李啟為.jpg"></div>

                     <div class="col-md-4"><img class="img-responsive" src="./images/people/王凡.jpg"></div>

                     <div class="col-md-4"><img class="img-responsive" src="./images/people/林湧達.jpg"></div>

                     <img class="nohover" id="TaiwanLogo" src="images/TaiwanLogo.png">

                     <a class="pointer-cursor" scroll-to="Result">

                               <i class="icon-beaker icon-light"></i>

                         <div> Result </div>

                         <li><img src="./images/MEETING/Meeting-(1).jpg"  ></li>

                         <li><img src="./images/MEETING/Meeting-(2).jpg"  ></li>

                         <li><img src="./images/MEETING/Meeting-(3).jpg"  ></li>

                         <li><img src="./images/MEETING/Meeting-(4).jpg"  ></li>

                         <li><img src="./images/MEETING/Meeting-(5).jpg"  ></li>

                         <li><img src="./images/MEETING/Meeting-(6).jpg"  ></li>

                         <li><img src="./images/MEETING/Meeting-(7).jpg"  ></li>

                         <li><img src="./images/MEETING/Meeting-(8).jpg"  ></li>

                         <li><img src="./images/MEETING/Meeting-(9).jpg"  ></li>

                         <li><img src="./images/MEETING/Meeting-(10).jpg" ></li>

                         <li><img src="./images/MEETING/Meeting-(11).jpg" ></li>

                         <li><img src="./images/MEETING/Meeting-(12).jpg" ></li>

                         <li><img src="./images/MEETING/Meeting-(13).jpg" ></li>

                         <li><img src="./images/MEETING/Meeting-(14).jpg" ></li>

                         <li><img src="./images/MEETING/Meeting-(15).jpg" ></li>

                         <li><img src="./images/MEETING/Meeting-(16).jpg" ></li>

                         <li><img src="./images/MEETING/Meeting-(17).jpg" ></li>

                         <li><img src="./images/MEETING/Meeting-(18).jpg" ></li>

                         <li><img src="./images/MEETING/Meeting-(19).jpg" ></li>

                         <li><img src="./images/MEETING/Meeting-(20).jpg" ></li>

                         <li><img src="./images/MEETING/Meeting-(21).jpg" ></li>

                         <li><img src="./images/MEETING/Meeting-(22).jpg" ></li>

                         <li><img src="./images/MEETING/Meeting-(23).jpg" ></li>

                         <li><img src="./images/MEETING/Meeting-(24).jpg" ></li>

                         <li><img src="./images/MEETING/Meeting-(25).jpg" ></li>

                         <li><img src="./images/MEETING/Meeting-(26).jpg" ></li>

                         <li><img src="./images/MEETING/Meeting-(27).jpg" ></li>

                         <li><img src="./images/MEETING/Meeting-(28).jpg" ></li>

                         <li><img src="./images/MEETING_2/MEETING_1.jpg"  ></li>

                         <li><img src="./images/MEETING_2/MEETING_2.jpg"  ></li>

                         <li><img src="./images/MEETING_2/MEETING_3.jpg"  ></li>

                         <li><img src="./images/MEETING_2/MEETING_4.jpg"  ></li>

                         <li><img src="./images/MEETING_2/MEETING_5.jpg"  ></li>

                         <li><img src="./images/MEETING_2/MEETING_6.jpg"  ></li>

                         <li><img src="./images/MEETING_2/MEETING_7.jpg"  ></li>

                         <li><img src="./images/AZALEA/(1).jpg"  ></li>

                         <li><img src="./images/AZALEA/(2).jpg"  ></li>

                         <li><img src="./images/AZALEA/(3).jpg"  ></li>

                         <li><img src="./images/AZALEA/(4).jpg"  ></li>

                         <li><img src="./images/AZALEA/(5).jpg"  ></li>

                         <li><img src="./images/AZALEA/(6).jpg"  ></li>

                         <li><img src="./images/AZALEA/(7).jpg"  ></li>

                         <li><img src="./images/AZALEA/(8).jpg"  ></li>

                         <li><img src="./images/AZALEA/(9).jpg"  ></li>

                         <li><img src="./images/AZALEA/(10).jpg" ></li>

                         <li><img src="./images/AZALEA/(11).jpg" ></li>

                         <li><img src="./images/AZALEA/(12).jpg" ></li>

                         <li><img src="./images/AZALEA/(13).jpg" ></li>

                         <li><img src="./images/AZALEA/(14).jpg" ></li>

                 <p class="header">R.g.</p>

                     &nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbspAccording to Jie Tao and his colleague’s study, agricultural and forestry residues can be used as an alternative of carbon source, taking advantage of <i><u>Rhodotorula glutinis’s</u></i> ability to assimilate xylose.  Agricultural residues such as rice straw and corn stalk are usually burned after harvest. Using these materials not only lower the expense but also benefits the environment. Raw materials like rice or wheat straw are first cut into pieces of appropriate size. They are then hydrolyzed using sulfuric acid with boiling water bath, turninig into hemicellulosic hydrolyzate. Saccharides can be obtained in supernatant after centrifugation and washings of the residue with hot water. <br/>

                     &nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbsp&nbspAfter expressing SrUCP in <i><u>Rhodotorula glutinis</u></i>, we will test for appropriate concentration for Agricultural residue extraction considering growth condition and heating power. The optimized heat power will then be used in large scale simulation as well as calculation of cost reduced. If time permitted, we will dig further into the component of the hemicellulosic hydrolyzate, as it is reviewed that there might be inhibiting compound.<br/>