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Team:Paris Saclay/Labwork
From 2013.igem.org
(Difference between revisions)
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* Digestion of Bba_K1155001 by EcoRI/PstI and SacII to check the size of fragments | * Digestion of Bba_K1155001 by EcoRI/PstI and SacII to check the size of fragments | ||
* PCR of digestion of Bba_K1155001 | * PCR of digestion of Bba_K1155001 | ||
| + | |||
| + | 07/16/13 | ||
| + | * Electrophoresis of the digestion of Bba_K1155001 [[File:Psright.png|20px]] | ||
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* Digestion of Bba_K1155002 by EcoRI/PstI and SacII to check the size of fragments | * Digestion of Bba_K1155002 by EcoRI/PstI and SacII to check the size of fragments | ||
* PCR of digestion of Bba_K1155002 | * PCR of digestion of Bba_K1155002 | ||
| + | |||
| + | 07/16/13 | ||
| + | * Electrophoresis of the digestion of Bba_K1155002 [[File:Pswrong.png|20px]] | ||
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07/15/13 | 07/15/13 | ||
* Liquid culture of Bba_B0015, Bba_B0017 [[File:Psright.png|20px]] | * Liquid culture of Bba_B0015, Bba_B0017 [[File:Psright.png|20px]] | ||
| + | |||
| + | 07/16/13 | ||
| + | * Extraction of Bba_B0015, Bba_B0017 from DH5α | ||
| + | * Digestion of Bba_B0015, Bba_B0017, Bba_I732019 by EcoRI/PstI | ||
| Line 148: | Line 158: | ||
07/15/13 | 07/15/13 | ||
* Liquid culture of Bba_B0015, Bba_B0017, Bba_I732019 [[File:Psright.png|20px]] | * Liquid culture of Bba_B0015, Bba_B0017, Bba_I732019 [[File:Psright.png|20px]] | ||
| + | |||
| + | 07/16/13 | ||
| + | * Extraction of Bba_B0015, Bba_B0017, Bba_I732019 from DH5α | ||
| + | * Digestion of Bba_B0015, Bba_B0017, Bba_I732019 by EcoRI/PstI | ||
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* Digestion of BphR2 in PSB1C3 by EcoRI/PstI and SacII to check the size of fragments | * Digestion of BphR2 in PSB1C3 by EcoRI/PstI and SacII to check the size of fragments | ||
* PCR of digestion of BphR2 in PSB1C3 | * PCR of digestion of BphR2 in PSB1C3 | ||
| + | |||
| + | 07/16/13 | ||
| + | * Electrophoresis of the digestion of BphR2 in PSB1C3 [[File:Pswrong.png|20px]] | ||
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* Digestion of Bba_J04450 to chek the size for the plasmid | * Digestion of Bba_J04450 to chek the size for the plasmid | ||
* Electrophoresis of the digestion of Bba_J04450 [[File:Psright.png|20px]] | * Electrophoresis of the digestion of Bba_J04450 [[File:Psright.png|20px]] | ||
| + | |||
| + | 07/16/13 | ||
| + | * Digestion of PSB3K3 by EcoRI/PstI | ||
| + | * Electrophoresis of the digestion of PSB3K3 by EcoRI/PstI [[File:Psright.png|20px]] | ||
| + | * Gel purification of electrophoresis of the digestion of PSB3K3 by EcoRI/PstI | ||
{{Team:Paris_Saclay/incl_fin}} | {{Team:Paris_Saclay/incl_fin}} | ||
Revision as of 19:53, 26 September 2013
Contents |
Labwork overview
Bba_K1155000
07/01/13
- Digestion of PSB1C3 plasmid and PCR products : Pfnr by EcoRI/ PstI
- Ligation of PSB1C3 and Pfnr
07/02/13
- Tranformation of Bba_K1155000 in DH5α
07/03/13
- Colony PCR of Bba_K1155000 to check good insertion of Pfnr in PSB1C3
- New culture of Bba_K1155000
07/04/13
- Estimation of Pfnr Colony PCR size fragments thanks to Gene manager
- Electrophoresis of Colony PCR products
- Stock of Bba_K1155000
- Culture of Bba_K1155000
- Extraction of Bba_K1155000 from DH5α
- Digestion of Bba_K1155000 by NotI, MluI, HpaI to check good insertion of Pfnr in PSB1C3 again
07/05/13
- Electrophoresis of the digestion of Bba_K1155000 by NotI, HpaI, MluI
- Extraction of Bba_K1155000 from DH5α
07/15/13
- Sequensis analysis of Bba_K1155000
WE OBTAIN OUR BIOBRICK
Bba_K1155001
07/09/13
- PCR purification of BphR1
- Digestion PCR products : BphR1 and digestion of PSB1C3
- Denaturation of EcoRI/PstI used for the digestion of PCR products : BphR1 and PSB1C3
- Ligation of BphR1 and PSB1C3
07/10/13
- Transformation of ligation of PSB1C3 and BphR1
07/11/13
- Colony PCR of Bba_K1155001 in DH5α to check good insertion of BphR1 in PSB1C3
- Electrophoresis of Colony PCR products : Bba_K1155001 in PSB1C3
07/12/13
- Stock of clones 5, 6 for Bba_K1155001
- Extraction of clones 5, 6 for Bba_K1155001 from DH5α
07/15/13
- Digestion of Bba_K1155001 by EcoRI/PstI and SacII to check the size of fragments
- PCR of digestion of Bba_K1155001
07/16/13
- Electrophoresis of the digestion of Bba_K1155001
Bba_K1155002
07/09/13
- PCR purification of BphA1
- Digestion PCR products : BphA1 and digestion of PSB1C3
- Denaturation of EcoRI/PstI used for the digestion of PCR products : BphA1 and PSB1C3
- Ligation of BphA1 and PSB1C3
07/10/13
- Transformation of ligation of PSB1C3 and BphA1
07/11/13
- Colony PCR of Bba_K1155002 in DH5α to check good insertion of BphA1 in PSB1C3
- Electrophoresis of Colony PCR products : Bba_K1155002 in PSB1C3
07/12/13
- Stock of clones 5, 6, 7, 8 for Bba_K1155002
- Streak of Bba_K1155002
- Extraction of clones 5, 6, 7, 8 for Bba_K1155002 from DH5α
07/15/13
- Digestion of Bba_K1155002 by EcoRI/PstI and SacII to check the size of fragments
- PCR of digestion of Bba_K1155002
07/16/13
- Electrophoresis of the digestion of Bba_K1155002
Bba_K1155003
07/02/13
- Tranformation of Bba_K592009 in DH5α
07/03/13
- New culture of Bba_K592009
07/05/13
- Extraction of Bba_K592009 from DH5α
07/08/13
- Digestion of Bba_K592009 by NotI, XhoI, EcoRI, EcoRI/PstI
- Electrophoresis of the digestion of Bba_K592009 by NotI, XhoI, EcoRI, EcoRI/PstI
07/12/13
- Transformation of Bba_B0015, Bba_B0017
- Design of RBS-AmilCP oligos
07/15/13
- Liquid culture of Bba_B0015, Bba_B0017
07/16/13
- Extraction of Bba_B0015, Bba_B0017 from DH5α
- Digestion of Bba_B0015, Bba_B0017, Bba_I732019 by EcoRI/PstI
Bba_K1155004
Bba_K1155005
Bba_K1155006
Bba_K1155007
07/02/13
- Tranformation of Bba_I732017 in DH5α
07/03/13
- New culture of Bba_I732017
07/05/13
- Extraction of Bba_I732017 from DH5α
07/08/13
- Digestion of Bba_I732017 by NotI, XhoI, EcoRI, EcoRI/PstI
- Electrophoresis of the digestion of Bba_I732017 by NotI, XhoI, EcoRI, EcoRI/PstI
07/10/13
- Transformation of Bba_I732019 in DH5α
07/12/13
- Transformation of Bba_I732019, Bba_B0015, Bba_B0017
07/15/13
- Liquid culture of Bba_B0015, Bba_B0017, Bba_I732019
07/16/13
- Extraction of Bba_B0015, Bba_B0017, Bba_I732019 from DH5α
- Digestion of Bba_B0015, Bba_B0017, Bba_I732019 by EcoRI/PstI
Bba_K1155008
Bba_K1155009
07/01/13
- Design of oligos for amplification of BphR2 gene of Pseudomonas pseudoalcaligenes, Pseudomonas oleovorans
07/09/13
- PCR purification of BphR2
- Digestion PCR products : BphR2 and digestion of PSB1C3
- Denaturation of EcoRI/PstI used for the digestion of PCR products : BphR2 and PSB1C3
- Ligation of BphR2 and PSB1C3
07/10/13
- Transformation of ligation of PSB1C3 and BphR2
07/11/13
- Colony PCR of BphR2 in PSB1C3 in DH5α to check good insertion of BphR2 in PSB1C3
- Electrophoresis of Colony PCR products : BphR2 in PSB1C3
07/12/13
- Stock of clones 3, 4 for BphR2
- Streak of BphR2
- Extraction of clones 3, 4 for BphR2 from DH5α
07/15/13
- Digestion of BphR2 in PSB1C3 by EcoRI/PstI and SacII to check the size of fragments
- PCR of digestion of BphR2 in PSB1C3
07/16/13
- Electrophoresis of the digestion of BphR2 in PSB1C3
PSB3K3
07/09/13
- Transformation of Bba_J04450 in DH5α
07/10/13
- Mini and maxi preparation of the culture of Bba_J04450
07/11/13
- Extraction of Bba_J04450 from DH5α
- Digestion of Bba_J04450 to chek the size for the plasmid
- Electrophoresis of the digestion of Bba_J04450
07/16/13
- Digestion of PSB3K3 by EcoRI/PstI
- Electrophoresis of the digestion of PSB3K3 by EcoRI/PstI
- Gel purification of electrophoresis of the digestion of PSB3K3 by EcoRI/PstI
