Template:Team:Uppsala/JS/notebook

From 2013.igem.org

(Difference between revisions)
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  else if(id == 'd2013629')
  else if(id == 'd2013629')
  {
  {
-
  ds = '<div id="dairy-text"><h1>Saturday 2013-06-29</h1><br><p><b>Name of participants:</b> Magnus B, Alexander W, Nils A, Anders E,'+
+
  ds = '<div id="dairy-text"><h1>Saturday 2013-06-29</h1><br><p><b>Name of participants:</b> Magnus B, Alexander W, Nils'+ 'A, Anders E, Mikael S<br><br>'+
-
'Mikael S, Lovisa P., Emil M., Peter C., Ken B.-A., Karl H.<br><br>'+
+
'<h2>Ongoing constructs:</h2>'+
-
'<h2>Ongoing constructs:</h2>'+
+
'<b>L. reuteri:</b><br>'+
-
'<b>L. reuteri:</b><br>'+
+
'1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>'+
-
'1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>'+
+
'8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>'+
-
'8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>'+
+
'10. 256-noplasmid<br>11. 36E-noplasmid<br><br>'+
-
'10. 256-noplasmid<br>11. 36E-noplasmid<br><br>'+
+
'<b>E. faecalis:</b><br>'+
-
'<b>E. faecalis:</b><br>'+
+
'9. JH2-2 pAMβ1<br><br>'+
-
'9. JH2-2 pAMβ1<br><br>'+
+
'<b>L. lactis:</b><br>'+
-
'<b>L. lactis:</b><br>'+
+
'13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br>'+
-
'13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br>'+
+
'<b>E.coli (strain D5α):</b><br>'+
-
'<b>E.coli (strain D5α):</b><br>'+
+
'19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>'+
-
'3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>7. pSB3K3-red<br>11. pSB4A15<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>'+
+
'23. pSB1C3-CrtB<br>24.B0034-CrtI<br>38. pEL3A16-red<br>42. pSB4S15-red<br>57.B0034-Idi<br>75. pEL3A15-CrtB<br>'+
-
'19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>'+
+
'76. pEL3K16-CrtI<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>'+
-
'23. pSB1C3-CrtB<br>24.B0034-CrtI<br>38. pEL3A16-red<br>42. pSB4S15-red<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>'+
+
'66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>'+
-
'45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>57.B0034-Idi<br>75. pEL3A15-CrtB<br>'+
+
'71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15<br><br>'+
-
'76. pEL3K16-CrtI<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>'+
+
'<h2>Todays work</h2>'+
-
'66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>'+
+
'<b>PCR purification:</b><br>'+
-
'71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15<br><br>'+
+
'CP1<br>CP8<br>CP11<br>CP29<br>CP30<br>CP41<br>Cp44<br><br>'+
-
'<h2>Todays work</h2>'+
+
'<b>Digestion:</b><br>'+
-
'<b>PCR purification:</b><br>'+
+
'27. pEL3K16-red<br>51.pEL3C18-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>'+
-
'CP1<br>CP8<br>CP11<br>CP29<br>CP30<br>CP41<br>Cp44<br><br>'+
+
'66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>'+
-
'<b>Digestion:</b><br>'+
+
'71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>'+
-
'27. pEL3K16-red<br>51.pEL3C18-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>'+
+
'77. pSB4C15 (Assembly of 42. pSB4S15 and precut CmR)<br><br>'+
-
'66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>'+
+
'<b>Ligation:</b><br>'+
-
'71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>'+
+
'24.3 B0034-CrtI -> 27. pEL3K16-red<br>23.2 pSB1C3-CrtB -> 38. pEL3A16-red<br><br>'+
-
'77. pSB4C15 (Assembly of 42. pSB4S15 and precut CmR)<br><br>'+
+
'<b>Transformation:</b><br>'+
-
'<b>Ligation:</b><br>'+
+
'75. pEL3A15 – CrtB*<br>76. pEL3K16 – CrtI* <br>*Cancelled, wrong solution was used -> redo tomorrow.<br><br>'+
-
'24.3 B0034-CrtI -> 27. pEL3K16-red<br>23.2 pSB1C3-CrtB -> 38. pEL3A16-red<br><br>'+
+
'<b>Re-streak:</b><br>'+
-
'<b>Transformation:</b><br>'+
+
'1.2 pSB1C3-red (from frozen stock)<br>7. pSB3K3 (”)<br>60. PK401 dam<br><br>'+
-
'75. pEL3A15 – CrtB*<br>76. pEL3K16 – CrtI* <br>*Cancelled, wrong solution was used -> redo tomorrow.<br><br>'+
+
'<b>Plasmid preparation:</b><br>'+
-
'<b>Re-streak:</b><br>'+
+
'42. pSB4S15-red<br><br>'+
-
'1.2 pSB1C3-red (from frozen stock)<br>7. pSB3K3 (”)<br>60. PK401 dam<br><br>'+
+
'<b>O/N Screening PCR:</b><br>'+
-
'<b>Plasmid preparation:</b><br>'+
+
'25. pSB1C3-CrtY<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>'+
-
'3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>7. pSB3K3-red<br>11. pSB4A15<br>42. pSB4S15-red<br><br>'+
+
'(19,20,21(with DMSO dilution series(7,9,12))<br><br>'+
-
'<b>O/N Screening PCR:</b><br>'+
+
'<b>PCR (screening?):</b><br>'+
-
'25. pSB1C3-CrtY<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>'+
+
'57.B0034-Idi<br>IspA<br><br>'+
-
'(19,20,21(with DMSO dilution series(7,9,12))<br><br>'+
+
'<b>Gel electrophoresis:</b><br>'+
-
'<b>PCR (screening?):</b><br>'+
+
'57.B0034-Idi<br>IspA<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>'+
-
'57.B0034-Idi<br>IspA<br><br>'+
+
'21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>38. pEL3A16-red (digest)<br>23.1 pSB1C3-CrtB (digest)<br>23.2 pSB1C3-CrtB (digest)<br>'+
-
'<b>Gel electrophoresis:</b><br>'+
+
'27. pEL3K16-red (digest)<br>24.3 B0034-CrtI (digest)<br>24.4 B0034-CrtI (digest)<br><br>'+
-
'43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>'+
+
'<h2>Results</h2>'+
-
'48. pSB1C3-B0034-His-STS<br>57.B0034-Idi<br>IspA<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>'+
+
'<b>Today:</b><br>'+
-
'21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>38. pEL3A16-red (digest)<br>23.1 pSB1C3-CrtB (digest)<br>23.2 pSB1C3-CrtB (digest)<br>'+
+
'<b>Plasmid preparation:</b><br>'+
-
'27. pEL3K16-red (digest)<br>24.3 B0034-CrtI (digest)<br>24.4 B0034-CrtI (digest)<br><br>'+
+
'42.1 pSB4S15-red klon 1: 27.4 ng/µL<br>42.2 pSB4S15-red klon 2: 21.8 ng/µL<br>42.3 pSB4S15-red klon 3: 24.3 ng/µL<br>'+
-
'<h2>Results</h2>'+
+
'42.4 pSB4S15-red klon 4: 25.3 ng/µL<br><br>'+
-
'<b>Today:</b><br>'+
+
'<b>Gel electrophoresis:</b><br>'+
-
'<b>Plasmid preparation:</b><br>'+
+
'57.B0034-Idi: still not good after three attempts<br>IspA: eventually good<br>38. pEL3A16-red (digest): failed, redo subcloning tomorrow <br>'+
-
'42.1 pSB4S15-red klon 1: 27.4 ng/µL<br>42.2 pSB4S15-red klon 2: 21.8 ng/µL<br>42.3 pSB4S15-red klon 3: 24.3 ng/µL<br>'+
+
'23.1 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow<br>23.2 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow<br>'+
-
'42.4 pSB4S15-red klon 4: 25.3 ng/µL<br><br>'+
+
'27. pEL3K16-red (digest): succeded <br>24.3 B0034-CrtI (digest): failed, redo subcloning tomorrow<br>'+
-
'<b>Gel electrophoresis:</b><br>'+
+
'24.4 B0034-CrtI (digest): failed, redo subcloning tomorrow<br><br>'+
-
'43. pSB1C3-B0034-TAL: failed (image 1 2013.06.28)<br>44. pSB1C3-B0034-His-TAL: failed (“)<br>45. pSB1C3-B0034-4CL: failed (“)<br>'+
+
'<b>Transformation:</b><br>'+
-
'46. pSB1C3-B0034-His-4CL: failed (“)<br>47. pSB1C3-B0034-STS: failed (image 2 2013.06.28)<br>48. pSB1C3-B0034-His-STS: failed (“)<br>'+
+
'75. pEL3A15 - CrtB failed<br><br>'+
-
'57.B0034-Idi: still not good after three attempts<br>IspA: eventually good<br>38. pEL3A16-red (digest): failed, redo subcloning tomorrow <br>'+
+
'<b>PCR purification (of promotors):*</b><br>'+
-
'23.1 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow<br>23.2 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow<br>'+
+
'CP1: 40 ng/µl<br>CP8: 41,3 ng/µl<br>CP11: 46,7 ng/µl<br>CP29: 40 ng/µl<br>CP30: 58 ng/µl<br>CP41: 41,9 ng/µl<br>Cp44: 42,3 ng/µl<br><br>'+
-
'27. pEL3K16-red (digest): succeded <br>24.3 B0034-CrtI (digest): failed, redo subcloning tomorrow<br>'+
+
'*PCR-purification of the promotors may have washed away the promotors themselves, as they are almost as short as the primers that purification '+
-
'24.4 B0034-CrtI (digest): failed, redo subcloning tomorrow<br><br>'+
+
'is meant to remove, on the other hand, the DNA-concentration of the purified product was measured to around 40 ng/µl. → redo all promotor '+
-
'<b>Transformation:</b><br>'+
+
'constructs (not 77) from annealing of R and F (without PCR-cleaning). Everything left in PCR-machine, maybe the digested plasmids and '+
-
'75. pEL3A15 - CrtB failed<br><br>'+
+
'B0032-BFP-parts can be used.<br><br>'+
-
'<b>PCR purification (of promotors):*</b><br>'+
+
'<b>Previous day:</b><br>'+
-
'CP1: 40 ng/µl<br>CP8: 41,3 ng/µl<br>CP11: 46,7 ng/µl<br>CP29: 40 ng/µl<br>CP30: 58 ng/µl<br>CP41: 41,9 ng/µl<br>Cp44: 42,3 ng/µl<br><br>'+
+
'<b>ON screening:</b><br>'+
-
'*PCR-purification of the promotors may have washed away the promotors themselves, as they are almost as short as the primers that purification '+
+
'19. pSB4C5-CrtE~I /w RBS, Linker & ZF: failed -> redo today with dilutions<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: failed -> redo today with dilutions<br>'+
-
'is meant to remove, on the other hand, the DNA-concentration of the purified product was measured to around 40 ng/µl. → redo all promotor '+
+
'21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed: -> redo today with dilutions<br><br></p></div>';
-
'constructs (not 77) from annealing of R and F (without PCR-cleaning). Everything left in PCR-machine, maybe the digested plasmids and '+
+
-
'B0032-BFP-parts can be used.<br><br>'+
+
-
'<b>Previous day:</b><br>'+
+
-
'<b>ON screening:</b><br>'+
+
-
'19. pSB4C5-CrtE~I /w RBS, Linker & ZF: failed -> redo today with dilutions<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: failed -> redo today with dilutions<br>'+
+
-
'21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed: -> redo today with dilutions<br><br></p></div>';
+
  }
  }
   else if(id == 'd201373')
   else if(id == 'd201373')

Revision as of 18:37, 26 September 2013

Retrieved from "http://2013.igem.org/Template:Team:Uppsala/JS/notebook"