"
Team:Carnegie Mellon/Project/Procedure
From 2013.igem.org
(Difference between revisions)
Enpederson (Talk | contribs) |
Enpederson (Talk | contribs) |
||
| Line 3: | Line 3: | ||
<br> | <br> | ||
<h1>Procedure</h1> | <h1>Procedure</h1> | ||
| + | <p>The basic goals of the project include the following: | ||
| + | <ol> | ||
| + | <li>Engineer a temperate phage (specifically λ) to synthesize KillerRed in <i>E. coli</i></li> | ||
| + | <li>Demonstrate KillerRed's ability to kill <i>E. coli</i></li> | ||
| + | |||
| + | |||
| + | </o> | ||
</html> | </html> | ||
[[image:Plasmid Construct.png|thumb|600px|center|The plasmid construct we use for expression consists of the <partinfo>BBa_R0010</partinfo> lac promoter, the <partinfo>BBa_B0034</partinfo> 100% efficiency RBS standard and either the KillerRed BBa_K1184000 coding sequence or mRFP1 <partinfo>BBa_E1010</partinfo> coding sequence. ]] | [[image:Plasmid Construct.png|thumb|600px|center|The plasmid construct we use for expression consists of the <partinfo>BBa_R0010</partinfo> lac promoter, the <partinfo>BBa_B0034</partinfo> 100% efficiency RBS standard and either the KillerRed BBa_K1184000 coding sequence or mRFP1 <partinfo>BBa_E1010</partinfo> coding sequence. ]] | ||
Revision as of 00:03, 27 September 2013
Procedure
The basic goals of the project include the following:
- Engineer a temperate phage (specifically λ) to synthesize KillerRed in E. coli
- Demonstrate KillerRed's ability to kill E. coli
This is meant to be a guide to our experimental process, including challenges we encountered from conception to results. For a detailed description of our protocols and methods we used, visit our Protocols and Notebook pages.
