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Team:Washington/60mmPetriDishAssay
From 2013.igem.org
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Revision as of 04:38, 27 September 2013
60 mm Petri Dish Assay 1. Add 7.5 ml of the overnight culture prepared according to the general protocol above into two 60 mm petri dishes. 2. Cover one of the 60 mm petri dishes with foil. 3. Secure the foiled petri dish with tape on top of the orbital shaker. 4. Take another 60 mm petri dish (spacer plate) and place it below the uncovered petri dish. 5. Place the stacked plates such that the bottom of the spacer plate aligns with the lighted circle in the app. 6. Secure the plates with tape. Incubate the tablet and dishes on an orbital shaker (RPM?) for 16 hours at 37C 7. Transfer 200 ul culture to a 96-well black, clear-bottom plate for analysis. 8. Follow the Fluorescent Analysis Protocol.
