Template:Kyoto/Notebook/Sep 27

From 2013.igem.org

(Difference between revisions)
(→Sep 27)
(→Sep 27)
Line 140: Line 140:
|}
|}
[[File:igku_xxxxxx.xxx]]<br>
[[File:igku_xxxxxx.xxx]]<br>
 +
===Miniprep===
 +
<div class="experiment">
 +
<span class="author">No name</span>
 +
{|class="wikitable"
 +
!DNA||concentration[&micro;g/mL]||260/280||260/230
 +
|-
 +
|Pcon-aptamer-DT
 +
|-
 +
|Pcon-spinach-DT
 +
|-
 +
|Pcon-antisense
 +
|-
 +
|Pcon-tetR-DT
 +
|-
 +
|Pcon-attenuator-DT
 +
|-
 +
|Pcon-antisense-spinach-DT
 +
|-
 +
|}

Revision as of 05:49, 27 September 2013

Contents

Sep 27

Gel Extraction

じっけんしゃ

LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）

Gel Extraction

じっけんしゃ

LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）

Electrophoresis

じっけんしゃ

LaneSampleEnzyme1Enzyme2
（°Д°）

File:Igku xxxxxx.xxx

Gel Extraction

じっけんしゃ

LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）

Liquid Culture

No name

Samplemedium
(・ω・)
（・Ｘ・） 　

Ligation

じっけんしゃ

stateVectorInserterLigation High ver.2
experimentnamexx µLnamexx µLxx µL
NCnamexx µLnamexx µLxx µL


Liquid Culture

No name

Samplemedium
Pcon-spinach-DTplusgrow Amp
Pcon-tetRaptamer-DTplusgrow Amp　
Pcon-antisense-spinach-DTplusgrow Amp
spinach-DTplusgrow CP

RNA Extraction

Nameconcentration[µg/mL]260/280260/230
9/26 Pcon-GFP-DT423.01.831.46
9/26 Pcon-GFP-DT K 298.71.821.50

Electrophoresis

じっけんしゃ

LaneSampleEnzyme1Enzyme2
19/27 Pcon-GFP-DT(RNA)
21kb ladder
39/27 Pcon-GFP-DT K(RNA)

File:Igku xxxxxx.xxx

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
Pcon-aptamer-DT
Pcon-spinach-DT
Pcon-antisense
Pcon-tetR-DT
Pcon-attenuator-DT
Pcon-antisense-spinach-DT
Retrieved from "http://2013.igem.org/Template:Kyoto/Notebook/Sep_27"