Template:Kyoto/Notebook/Sep 27

From 2013.igem.org

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!state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2
!state||colspan="2"|Vector||colspan="2"|Inserter||Ligation High ver.2
|-
|-
-
|experiment||name||xx µL||name||xx µL||xx µL
+
|experiment||9/17 pSB1C3 (E+S) 10.0ng/µL||10µL||9/26 pT181 attenuator(E+S) 7.5ng/µL||1.3µL||xx µL
|-
|-
-
|NC||name||xx µL||name||xx µL||xx µL
+
|experiment||9/25 DT (E+X) 42.8ng/µL||2.3µL||9/22 Pcon-pT181 antisense(E+S) 46.7ng/µL||1.5µL||xx µL
 +
|-
 +
|experiment||9/27 pSB4K5(E+P) 12.9ng/µL||7.8µL||9/10 Pcon-attenuator(E+S) 13.8ng/µL||8.0µL||xx µL
 +
|-
 +
|experiment||9/27 pSB4K5(E+P) 12.9ng/µL||7.8µL||9/25 RBS-GFP-DT(X+P) 13.9ng/µL||12µL||xx µL
 +
|-
 +
|experiment||9/8 pSB4K5 (E+S) 17.6ng/µL||xx µL||8/21 Pcon-GFP-DT(E+S) 11ng/µL||12.6µL||xx µL
|}
|}
</div>
</div>
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===Liquid Culture===
===Liquid Culture===

Revision as of 06:19, 27 September 2013

Contents

Sep 27

Gel Extraction

Kojima

LaneDNAEnzyme
11kbp ladder--
2pSB1C3EcoRI+SpeI
3
4
6Pcon-attenuator-aptmer-DTEcoRI+SpeI
7
8
10Pcon-attenuator-aptmer-DTEcoRI+SpeI
11
12

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
Pcon-attenuator-DT(EcoRI+SpeI)6.81.980.43
pT181-attenuator(EcoRI+SpeI)7.51.840.06
pSB1C3(EcoRI+SpeI)17.71.970.71

Gel Extraction

じっけんしゃ

LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）

Electrophoresis

じっけんしゃ

LaneSampleEnzyme1Enzyme2
（°Д°）

File:Igku xxxxxx.xxx

Gel Extraction

じっけんしゃ

LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）

Liquid Culture

No name

Samplemedium
(・ω・)
（・Ｘ・） 　

Ligation

じっけんしゃ

stateVectorInserterLigation High ver.2
experiment9/17 pSB1C3 (E+S) 10.0ng/µL10µL9/26 pT181 attenuator(E+S) 7.5ng/µL1.3µLxx µL
experiment9/25 DT (E+X) 42.8ng/µL2.3µL9/22 Pcon-pT181 antisense(E+S) 46.7ng/µL1.5µLxx µL
experiment9/27 pSB4K5(E+P) 12.9ng/µL7.8µL9/10 Pcon-attenuator(E+S) 13.8ng/µL8.0µLxx µL
experiment9/27 pSB4K5(E+P) 12.9ng/µL7.8µL9/25 RBS-GFP-DT(X+P) 13.9ng/µL12µLxx µL
experiment9/8 pSB4K5 (E+S) 17.6ng/µLxx µL8/21 Pcon-GFP-DT(E+S) 11ng/µL12.6µLxx µL

Liquid Culture

No name

Samplemedium
Pcon-spinach-DTplusgrow Amp
Pcon-tetRaptamer-DTplusgrow Amp　
Pcon-antisense-spinach-DTplusgrow Amp
spinach-DTplusgrow CP

RNA Extraction

Nameconcentration[µg/mL]260/280260/230
9/26 Pcon-GFP-DT423.01.831.46
9/26 Pcon-GFP-DT K 298.71.821.50

Electrophoresis

じっけんしゃ

LaneSample
19/27 Pcon-GFP-DT(RNA)
21kb ladder
39/27 Pcon-GFP-DT K(RNA)

File:Igku xxxxxx.xxx

Miniprep

No name

DNA
Pcon-aptamer-DT
Pcon-spinach-DT
Pcon-antisense
Pcon-tetR-DT
Pcon-attenuator-DT
Pcon-antisense-spinach-DT

Transformation

No name

Name1Name2Sample1(µL)Sample2(µL)Competent Cells(µL)Total(µL)
Pcon-attenuator+RBS-GFP-DT(4K5)Pcon-attenuator-DT(1A2)213033
Pcon-attenuator+RBS-GFP-DT(4K5)Pcon-antisense-spinach-DT(1A2)213033
Pcon-attenuator+RBS-GFP-DT(4K5)Pcon-tetR aptamer-DT(1A2)213033
Pcon-RBS-GFP-DT(4K5)Pcon-attenuator-DT(1A2)213033
Pcon-RBS-GFP-DT(4K5)Pcon-antisense-spinach-DT(1A2)213033
Pcon-RBS-GFP-DT(4K5)Pcon-tetR aptamer-DT(1A2)213033
Pcon-attenuator+RBS-GFP-DT(4K5)--2--2022
Pcon-RBS-GFP-DT(4K5)--2--2022
attenuator(1C3)--2--2022
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