Team:UT Dallas/project part1

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Revision as of 19:57, 27 September 2013

CscR and FruR

FruR/CscR

We have chosen two inducible repressors for use in this project, FruR and CscR. Sucrose is a disaccharide composed of glucose and fructose. The sucrose is converted to lactic acid by S.Mutans which leads to plaque formation and tooth decay. We will be using operon repressors for both sucrose and fructose to detect the concentrations of both of them. By using the repressor systems, we can detect the formation of plaque and prevent the formation of cavities. The repressor systems can be added with nspC to break down the formation of biofilm.

We have chosen two repressors for use in this project:
FruR
CscR

How They Work
The FruR gene encodes a protein which normally binds to and represses the fructose operon. When fructose is present in the system, it binds to the FruR protein and prevents it from repressing the operon promoter.
CscR works in the similar way, but it will bind to sucrose instead.

What We Did

    We isolated these repressors, FruR and CscR, in order to create a construct to detect low levels of sucrose and high levels of fructose. By using the repressor systems, we can detect the formation of plaque and prevent the formation of cavities.

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 <br>
 <b>FruR/CscR</b><br>
-The mouth is very complex in regards to the molecules that enter it.  In order to selectively fight S. Mutans, we need to detect its presence in the mouth.  One of the ways involved detecting the changing levels of fructose and sucrose. Using operon repressors for both sucrose and fructose, we can detect the concentrations of both of them. <br>
+ We have chosen two inducible repressors for use in this project, FruR and CscR. Sucrose is a disaccharide composed of glucose and fructose. The sucrose is converted to lactic acid by S.Mutans which leads to plaque formation and tooth decay. We will be using operon repressors for both sucrose and fructose to detect the concentrations of both of them. By using the repressor systems, we can detect the formation of plaque and prevent the formation of cavities. The repressor systems can be added with nspC to break down the formation of biofilm.  <br>
 <br>
 We have chosen two repressors for use in this project:<br>
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 <b>What We Did</b><br>
-      We utilized these repressors, FruR and CscR, to create a construct to detect low levels of sucrose and high levels of fructose. By using the repressor systems, we can detect the formation of plaque and prevent the formation of cavities.<br><br>
+      We isolated these repressors, FruR and CscR, in order to create a construct to detect low levels of sucrose and high levels of fructose. By using the repressor systems, we can detect the formation of plaque and prevent the formation of cavities.<br><br>
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 <img src="http://i826.photobucket.com/albums/zz188/lana_khazma/fancy%20stuff/sucrstuff_zps30aefeaf.png" border="0" alt=" photo sucrstuff_zps30aefeaf.png"/><br>