Team:Goettingen/Team/Reporter

From 2013.igem.org

(Difference between revisions)
Line 48: Line 48:
<div style="display:inline-block;margin-left:20px;background:#fff" class="prev half" >
<div style="display:inline-block;margin-left:20px;background:#fff" class="prev half" >
<img src="https://static.igem.org/mediawiki/2013/c/c5/Goe-reporter-2.png" style="width:100%" />
<img src="https://static.igem.org/mediawiki/2013/c/c5/Goe-reporter-2.png" style="width:100%" />
-
<p><b>Figure 1b: </b> When there is c-di-AMP present, the c-di-AMP can act as ligand of the repressor gene DarR, activating tis function. DarR binds to its binding site, blocking the RNA polymerase, and therefore disrupt transcription. Then we are supposed to see no signal or reduced signal.</p></div>
+
<p><b>Figure 1b: </b> In the presence of c-di-AMP, c-di-AMP stimulates DNA-binding activity of DarR and the RNA polymerase cannot initiate transcription. The cells do not produce GFP and are therefore non-fluorescent.</p></div>
<br />
<br />
<p>Reference:</p>
<p>Reference:</p>

Revision as of 10:56, 27 September 2013





The beast and its Achilles heel:

 A novel target to fight multi-resistant pathogenic bacteria



Reporter Team

The activity of transcriptional regulators can be easily detected by monitoring the activity of a promoter that is fused to a reporter gene. Recently, in Mycobacterium smegmatis, a transcriptional repressor (DarR) was identified that is able to bind to a specific DNA sequence upon association with c-di-AMP. This led us to the idea of developing a screening system for potential drugs interfering with c-di-AMP biofunction. While many Gram-positive bacteria require c-di-AMP for their growth, this molecule is not synthesized by the Gram-negative bacterium E. coli. Thus, we intend to build a reporter system that is composed of existing and of novel biobricks that will be developed during the project (Figure 1).


Reference:

1. Zhang et al. (2013) DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in Mycobacterium smegmatis, J. Biol. Chem. 288:3085–3096

 

Previous Next