Team:BYU Provo/Notebook/Cholera - Enzymes/May-June

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Revision as of 15:00, 27 September 2013


Cholera - Enzyme Notebook May - June



Cholera Detection
March-April
May-June
July-August
September
Protocols

May 1 - May 14


We started contacting teams that have worked with Savinase and DspB. We started the initial work of purifying AmyA which was available at BYU. As well we started outlining the biofilm degradation assay using positive and negative controls.


Daily log


Winter1.JPG
May 15 - May 31


Continued our work of cloning AmyA and Savinase into E. coli. Ran initial biofilm degradation assays using urea as a positive control.


Daily log



Winter1.JPG
June 1 - June 14


We are still having issues with AmyA and Savinase. We are starting to fine tune our biofilm degradation assay.


Daily log



Winter1.JPG
June 15 - June 30


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Daily log



Winter1.JPG