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Team:UT Dallas/project part1
From 2013.igem.org
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| - | <span class="title_spans">CscR and FruR</span><br><br> | + | <br><span class="title_spans">CscR and FruR</span><br><br> |
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| - | <b>FruR/CscR</b><br> | + | <font size=5><b>FruR/CscR</b><br></font> |
We have chosen two inducible repressors for use in this project, FruR and CscR. Sucrose is a disaccharide composed of glucose and fructose. The sucrose is converted to lactic acid by S.Mutans which leads to plaque formation and tooth decay. We will be using operon repressors for both sucrose and fructose to detect the concentrations of both of them. By using the repressor systems, we can detect the formation of plaque and prevent the formation of cavities. The repressor systems can be added with nspC to break down the formation of biofilm. <br> | We have chosen two inducible repressors for use in this project, FruR and CscR. Sucrose is a disaccharide composed of glucose and fructose. The sucrose is converted to lactic acid by S.Mutans which leads to plaque formation and tooth decay. We will be using operon repressors for both sucrose and fructose to detect the concentrations of both of them. By using the repressor systems, we can detect the formation of plaque and prevent the formation of cavities. The repressor systems can be added with nspC to break down the formation of biofilm. <br> | ||
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CscR <br><br> | CscR <br><br> | ||
| - | <b>How They Work</b><br> | + | <b><font size=3>How They Work</font></b><br> |
The FruR gene encodes a protein which normally binds to and represses the fructose operon. When fructose is present in the system, it binds to the FruR protein and prevents it from repressing the operon promoter. <br> | The FruR gene encodes a protein which normally binds to and represses the fructose operon. When fructose is present in the system, it binds to the FruR protein and prevents it from repressing the operon promoter. <br> | ||
CscR works in the similar way, but it will bind to sucrose instead. <br><br> | CscR works in the similar way, but it will bind to sucrose instead. <br><br> | ||
| - | <b>What We Did</b><br> | + | <b><font size=3>What We Did</font></b><br> |
We isolated these repressors, FruR and CscR, in order to create a construct to detect low levels of sucrose and high levels of fructose. By using the repressor systems, we can detect the formation of plaque and prevent the formation of cavities.<br><br> | We isolated these repressors, FruR and CscR, in order to create a construct to detect low levels of sucrose and high levels of fructose. By using the repressor systems, we can detect the formation of plaque and prevent the formation of cavities.<br><br> | ||
<center> | <center> | ||
Revision as of 20:00, 27 September 2013
CscR and FruR
FruR/CscR
We have chosen two inducible repressors for use in this project, FruR and CscR. Sucrose is a disaccharide composed of glucose and fructose. The sucrose is converted to lactic acid by S.Mutans which leads to plaque formation and tooth decay. We will be using operon repressors for both sucrose and fructose to detect the concentrations of both of them. By using the repressor systems, we can detect the formation of plaque and prevent the formation of cavities. The repressor systems can be added with nspC to break down the formation of biofilm.
We have chosen two repressors for use in this project:
FruR
CscR
How They Work
The FruR gene encodes a protein which normally binds to and represses the fructose operon. When fructose is present in the system, it binds to the FruR protein and prevents it from repressing the operon promoter.
CscR works in the similar way, but it will bind to sucrose instead.
What We Did
We isolated these repressors, FruR and CscR, in order to create a construct to detect low levels of sucrose and high levels of fructose. By using the repressor systems, we can detect the formation of plaque and prevent the formation of cavities.
<img src="http://i826.photobucket.com/albums/zz188/lana_khazma/fancy%20stuff/sucrstuff_zps30aefeaf.png" border="0" alt=" photo sucrstuff_zps30aefeaf.png"/>
<img src="http://i826.photobucket.com/albums/zz188/lana_khazma/fancy%20stuff/fruRstuff_zpsaf5e8f5b.png" border="0" alt=" photo fruRstuff_zpsaf5e8f5b.png"/ width=500 >
