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Team:TMU-Tokyo/Notebook experiment/3rd
From 2013.igem.org
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| - | Transformation following plasmid. | + | Transformation following plasmid to <i>Escherichia coli</i> MG1655 red. |
<Table><Tr><Td>Fragment 1, 2, 3 - Ap</Td></Tr></Table> | <Table><Tr><Td>Fragment 1, 2, 3 - Ap</Td></Tr></Table> | ||
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<b>September 16th</b><Br> | <b>September 16th</b><Br> | ||
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| + | Transformation result.<Br> | ||
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| + | <IMG Src="https://static.igem.org/mediawiki/2013/1/15/TMU-Tokyo_0916_2.jpg"><Br> | ||
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Mini prep following plasmid. | Mini prep following plasmid. | ||
<Table><Tr><Td>Fragment 1, 2, 3 - Ap, 3 - Cm, Km-Blunt</Td></Tr></Table> | <Table><Tr><Td>Fragment 1, 2, 3 - Ap, 3 - Cm, Km-Blunt</Td></Tr></Table> | ||
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| + | PCR following fragment. | ||
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| + | <Table cellpadding="5"> | ||
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| + | <Td>Fragment</Td><Td>Fragment 1</Td><Td>Fragment 2</Td><Td>Fragment 3 - Ap</Td><Td>Fragment 3 - Cm</Td> | ||
| + | </Tr> | ||
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| + | <Td>Primer</Td><Td>lacA-IRR/IRL<Br>cynR-SaApR</Td><Td>araCHC<Br>araA-TcC</Td><Td>lacI-R(FRT)2<Br>mhpA-SaApR</Td><Td>p(SaApR)-lacIN<Br>mhpA-CmC6</Td> | ||
| + | </Tr> | ||
| + | <Tr> | ||
| + | <Td>Templete</Td><Td>IF2<Br>FS2</Td><Td>Fragment 2-C<Br>Fragment 2-D</Td><Td>pSG76A2</Td><Td>lacI<Br>FRT-Cm2</Td> | ||
| + | </Tr> | ||
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Revision as of 20:21, 27 September 2013
Experiment > 3rd week
September 15th
PCR result
1: Fragment 1, 2: Fragment 2, 3: Fragment 3 - Ap,
4: Fragment 3- Cm, 5: pBAD-fimE
Ligation fragment and vector.
| Plasmid name | Fragment 1-A+B | Fragment 2 | Fragment 3 - Ap |
| Fragment | Fragment 1-A Fragment 1-B | Fragment 2 | Fragment 3 - Ap |
| Vector | pSB1C3 | pSB1C3 | pSB1C3 |
Transformation following plasmid to Escherichia coli MG1655 red.
| Fragment 1, 2, 3 - Ap |
September 16th
Transformation result.
Mini prep following plasmid.
| Fragment 1, 2, 3 - Ap, 3 - Cm, Km-Blunt |
1: Fragment 1, 2: Fragment 3 - Ap,
3-5: Fragment 3 - Cm, 6-8: Km-Blunt
PCR following fragment.
| Fragment | Fragment 1 | Fragment 2 | Fragment 3 - Ap | Fragment 3 - Cm |
| Primer | lacA-IRR/IRL cynR-SaApR | araCHC araA-TcC | lacI-R(FRT)2 mhpA-SaApR | p(SaApR)-lacIN mhpA-CmC6 |
| Templete | IF2 FS2 | Fragment 2-C Fragment 2-D | pSG76A2 | lacI FRT-Cm2 |
September 17th
September 18th
September 19th
September 20th
September 21th
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