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Team:DTU-Denmark/Notebook/27 June 2013
From 2013.igem.org
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== Main purposes today == | == Main purposes today == | ||
| - | Run gels to verify [https://2013.igem.org/Team:DTU-Denmark/Notebook/26_June_2013 yesterdays] PCR-prodcuts. | + | Run gels to verify [https://2013.igem.org/Team:DTU-Denmark/Notebook/26_June_2013 yesterdays] PCR-prodcuts. Make new PCR to amplify the signal peptides and the backbone. |
==who were in the lab== | ==who were in the lab== | ||
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==Procedure== | ==Procedure== | ||
| + | Signal peptides on 4% gels 80V for 45 min. All other PCR-products on a 1% gel 100V for 45 min. | ||
| + | Two new PCRs with ramp from 70°C → 60°C and 60°C → 50°C. Sec signal peptide on 70°C → 60°C and TAT signal peptide with new primer set 2 and 3 respectively on program 60°C → 50°C. | ||
| + | [[File:27.06.13_Sec_and_TAT_signalP.jpg|thumb|left|The gel of the successful PCR-amp. of the two signal peptides]] | ||
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== Conclusion from today == | == Conclusion from today == | ||
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Revision as of 07:23, 28 June 2013
Contents |
208 lab
Main purposes today
Run gels to verify yesterdays PCR-prodcuts. Make new PCR to amplify the signal peptides and the backbone.
who were in the lab
Gosia, Henrike, Kristian
Procedure
Signal peptides on 4% gels 80V for 45 min. All other PCR-products on a 1% gel 100V for 45 min. Two new PCRs with ramp from 70°C → 60°C and 60°C → 50°C. Sec signal peptide on 70°C → 60°C and TAT signal peptide with new primer set 2 and 3 respectively on program 60°C → 50°C.
