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{

ds = '<div id="dairy-text"><h1>Monday 2013-06-24</h1> Name of participants: Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Viktor T, Viktor B, Mikael S, Anton B, Marcus H, Lovisa P, Emil M, Ken B-A, Karl H, Peter C, Alexander W., Malin B., Magnus B., Thorsteinn O., Niclas S., Pontus D. <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 L. lactis 13. MG1363-pJP059 E. coli: 14. pET13b-zifz:4cl-PBSII:STS 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m <h2>Todays work</h2> Plasmid preparation: Lb8. reuteri 100-23 pGT232 Lb9. faecalis JH2-2 pAMβ1 Digestion of construct: 14. pET13b-zifz:4cl-PBSII:STS 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m O/N (from frozen stock): Lb2. 1063 pLUL631 Lb6. DSM 20016 pLUL63TsA8 Lb7. DSM 20016 pGFP Lb8. 100-23 pGT232 Lb9. faecalis JH2-2 pAMβ1 PCR: 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT* Lb13. Lc. lactis MG1363 pJP059* *Colony-PCR. Cells might need lysing. Two samples from the same stock was resuspended in 100μl distilled water. Lyzosyme and buffer was added to one of the samples followed by heating to 95 degrees celsius. Transformation test of competent cells (D5alpha strain number 10) with plasmid preps: 1.1. pSB1C3-red 18.2. pSB1C3-CHS_m 40. pEL3A17-red <h2>Results</h2> Plasmid preparation: Lb8. Lc. lactis 100-23 pGT232 clone 2: 14.8 ng/μl Lb9. Lc. lactis JH2-2 pAMβ1 clone 1: 13.5 ng/μl Lb9. Lc. lactis JH2-2 pAMβ1 clone 2: 13.4 ng/μl Lb9. faecaliJH2-2 pAMβ1 clone 4: 0.5 ng/μl Plasmid concentrations were low on the plasmid preparations from today and the ones done on 22/6-13. New plasmid preparations will be done tomorrow on these strains from the O/N that were done today. <h2>Other experiments</h2> Primer stock preparation Dilution of sequencing primers: 3 tubes of 5 µM VF2-primer, 200 uL 3 tubes 5 µM VR-primer, 200 µL Re-measuring concentration of earlier plasmid preps: * 1.4 CF48-pLR581: 127.8 ng/µl 1.5 CF48-pLR581: 215.9 ng/µl 3.2 DSM 20016-pVs2: 201.2 ng/µl 3.3 DSM 20016-pVs2: 139.1 ng/µl 3.10 DSM 20016-pVs2: 183.5 ng/µl *We deduced that the low, and sometimes even negative, concentrations were caused by a bubble when loading the samples. Changes to protocol have been made with this in mind. Preparing M17-broth, 950 ml. (“) SOB (“) CCMB80 (20% glycerol, pH 6,8) Alliquoting dNTP, buffer, enzymes</div>'

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}

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else if(id == 'd2013623')

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{

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ds = '<div id="dairy-text"><h1>Sunday 2013-06-23</h1> Name of participants: Hampus E, Alexander V <h2>Ongoing constructs:</h2> L. reuteri: 4. 100-23-noplasmid L. plantarum: 5. 256-rifR-pAMβ1 E. coli: 23.pCrcSB1C3-Crt-B 24.pSB1C3-Crt-I 25. pSB1C3-CrtY <h2>Todays work</h2> Glycerol stock preparation: Lb4. reuteri 100-23 no plasmid Lb5. plantarum 256 rifR-Pamβ1 23.pCrcSB1C3-Crc-B 24.pSB1C3-Crc-I Restreak: 25. pSB1C3- crtY Plasmidpreparation 23.pCrcSB1C3-Crc-B 24.pSB1C3-Crc-I <h2>Result</h2> O/N: 25.pSB1C3-crtY: One of the overnights did not grow </div>'

}

else if(id == 'd201373')

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   {
 	ds = '<div id="dairy-text"><h1>Monday 2013-06-24</h1><br><b>Name of participants: </b>Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Viktor T, Viktor B, Mikael S, Anton B, Marcus H, Lovisa P, Emil M, Ken B-A, Karl H, Peter C, Alexander W., Malin B., Magnus B., Thorsteinn O., Niclas S., Pontus D.<br><br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>L. lactis</b><br>13. MG1363-pJP059<br><br><b>E. coli:</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m	<br><br><h2>Todays work</h2><br><b>Plasmid preparation:</b><br>Lb8. reuteri 100-23 pGT232<br>Lb9. faecalis JH2-2 pAMβ1<br><br><b>Digestion of construct:</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br><br><b>O/N (from frozen stock): </b><br>Lb2. 1063 pLUL631<br>Lb6. DSM 20016 pLUL63TsA8<br>Lb7. DSM 20016 pGFP<br>Lb8. 100-23 pGT232<br>Lb9. faecalis JH2-2 pAMβ1<br><br><b>PCR:</b><br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT*<br>Lb13. Lc. lactis MG1363 pJP059*<br>*Colony-PCR. Cells might need lysing. Two samples from the same stock was resuspended in 100μl distilled water. Lyzosyme and buffer was added to one of the samples followed by heating to 95 degrees celsius. <br><br><b>Transformation test of competent cells (D5alpha strain number 10) with plasmid preps: </b><br>1.1. pSB1C3-red<br>18.2. pSB1C3-CHS_m<br>40. pEL3A17-red<br><br><h2>Results</h2><br><b>Plasmid preparation:</b><br>Lb8. Lc. lactis 100-23 pGT232 clone 2: 14.8 ng/μl<br>Lb9. Lc. lactis JH2-2 pAMβ1 clone 1: 13.5 ng/μl<br>Lb9. Lc. lactis JH2-2 pAMβ1 clone 2: 13.4 ng/μl<br>Lb9. faecaliJH2-2 pAMβ1 clone 4: 0.5 ng/μl<br>Plasmid concentrations were low on the plasmid preparations from today and the ones done on 22/6-13. New plasmid preparations will be done tomorrow on these strains from the O/N that were done today.<br><br><h2>Other experiments</h2><br><b>Primer stock preparation</b><br><br><b>Dilution of sequencing primers: </b><br>3 tubes of 5 µM VF2-primer, 200 uL<br>3 tubes 5 µM VR-primer, 200 µL<br><br><b>Re-measuring concentration of earlier plasmid preps: *</b><br>1.4 CF48-pLR581: 127.8 ng/µl<br>1.5 CF48-pLR581: 215.9 ng/µl<br>3.2 DSM 20016-pVs2: 201.2 ng/µl<br>3.3 DSM 20016-pVs2: 139.1 ng/µl<br>3.10 DSM 20016-pVs2: 183.5 ng/µl<br>*We deduced that the low, and sometimes even negative, concentrations were caused by a bubble when loading the samples. Changes to protocol have been made with this in mind.<br><br><b>Preparing M17-broth, 950 ml.</b><br><b>(“) SOB</b><br><b>(“) CCMB80 (20% glycerol, pH 6,8)</b><br><b>Alliquoting dNTP, buffer, enzymes</b></div>'
+ }
+  else if(id == 'd2013623')
+ {
+	ds = '<div id="dairy-text"><h1>Sunday 2013-06-23</h1><br><b>Name of participants: </b>Hampus E, Alexander V<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>4. 100-23-noplasmid<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br><br><b>E. coli: </b><br>23.pCrcSB1C3-Crt-B<br>24.pSB1C3-Crt-I<br>25. pSB1C3-CrtY<br><br><h2>Todays work</h2><br><b>Glycerol stock preparation:</b><br>Lb4. reuteri 100-23 no plasmid<br>Lb5. plantarum 256 rifR-Pamβ1<br>23.pCrcSB1C3-Crc-B<br>24.pSB1C3-Crc-I<br><br><b>Restreak:</b><br>25. pSB1C3- crtY<br> <br><b>Plasmidpreparation </b><br>23.pCrcSB1C3-Crc-B<br>24.pSB1C3-Crc-I<br><br><h2>Result</h2><br><b>O/N:</b><br>25.pSB1C3-crtY: One of the overnights did not grow<br></div>'
   }
   else if(id == 'd201373')

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