Template:Team:Uppsala/JS/notebook

From 2013.igem.org

(Difference between revisions)
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  {
  {
ds = '<div id="dairy-text"><h1>Sunday 2013-06-23</h1><br><b>Name of participants: </b>Hampus E, Alexander W<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>4. 100-23-noplasmid<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br><br><b>E. coli: </b><br>23.pCrcSB1C3-Crt-B<br>24.pSB1C3-Crt-I<br>25. pSB1C3-CrtY<br><br><h2>Todays work</h2><br><b>Glycerol stock preparation:</b><br>Lb4. reuteri 100-23 no plasmid<br>Lb5. plantarum 256 rifR-Pamβ1<br>23.pCrcSB1C3-Crc-B<br>24.pSB1C3-Crc-I<br><br><b>Restreak:</b><br>25. pSB1C3- crtY<br> <br><b>Plasmidpreparation </b><br>23.pCrcSB1C3-Crc-B<br>24.pSB1C3-Crc-I<br><br><h2>Result</h2><br><b>O/N:</b><br>25.pSB1C3-crtY: One of the overnights did not grow<br></div>'
ds = '<div id="dairy-text"><h1>Sunday 2013-06-23</h1><br><b>Name of participants: </b>Hampus E, Alexander W<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>4. 100-23-noplasmid<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br><br><b>E. coli: </b><br>23.pCrcSB1C3-Crt-B<br>24.pSB1C3-Crt-I<br>25. pSB1C3-CrtY<br><br><h2>Todays work</h2><br><b>Glycerol stock preparation:</b><br>Lb4. reuteri 100-23 no plasmid<br>Lb5. plantarum 256 rifR-Pamβ1<br>23.pCrcSB1C3-Crc-B<br>24.pSB1C3-Crc-I<br><br><b>Restreak:</b><br>25. pSB1C3- crtY<br> <br><b>Plasmidpreparation </b><br>23.pCrcSB1C3-Crc-B<br>24.pSB1C3-Crc-I<br><br><h2>Result</h2><br><b>O/N:</b><br>25.pSB1C3-crtY: One of the overnights did not grow<br></div>'
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}
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else if(id == 'd2013622')
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{
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ds = '<div id="dairy-text"><h1>Saturday 2013-06-22<h1><br><b>Name of participants: </b>Nafisa B, Mikael S, Marcus H<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br><br><h2>Todays work</h2><br><b>Glycerol stock preparation: </b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red <br>Lb6. reuteri DSM 20016-pLUL63TsA8<br>Lb7. reuteri DSM 20016-pGFP<br>Lb8. reuteri 100-23-pGT232<br>Lb9. faecalis  JH2-2- pAMβ1<br>Lb10. plantarum 256-noplasmid<br>Lb11. plantarum 36E-noplasmid<br>Lb12. reuteri DSM 20016-noplasmid<br>Lb14. reuteri DSM 20016-pLUL631 (B?)<br><br><b>O/N: </b><br>Lb4. reuteri100-23-noplasmid<br>Lb5. plantarum 256-rifR-pAMβ1<br><br><b>Plasmid preparation:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red <br>Lb14. DSM 20016-pLUL631 (B?)<br>Lb6. DSM 20016-pLUL63TsA8<br>Lb7. DSM 20016-pGFP<br><br><h2>Results</h2><br><b>Plasmid preparation:</b><br><b>L. reuteri:</b><br>Lb14.1 (strain 14, clone 1) DSM 20016-pLUL631 (B?): 10.0 ng/μl<br>Lb14.2 DSM 20016-pLUL631 (B?): 13.6 ng/μl<br>Lb14.3 DSM 20016-pLUL631 (B?): 14.7 ng/μl<br>Lb14.4 DSM 20016-pLUL631 (B?): 28,4 ng/μl<br>Lb6.1 DSM 20016-pLUL63TsA8: 18.7 ng/μl<br>Lb6.2 DSM 20016-pLUL63TsA8: 14.4 ng/μl<br>Lb6.3 DSM 20016-pLUL63TsA8: 12.5 ng/μl<br>Lb6.4 DSM 20016-pLUL63TsA8: 27.7 ng/μl<br>Lb7.2 DSM 20016-pGFP: 29.9 ng/μl<br>Lb7.3 DSM 20016-pGFP: 16.9 ng/μl<br>Lb7.4 DSM 20016-pGFP: 10.9 ng/μl</div>'
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}
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  else if(id == 'd2013621')
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{
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ds = '<div id="dairy-text"><h1>Friday 2013-06-21</h1><br><b>Name of participants: </b>Stephanie H, Alona N, Anton B, Mikael S, Nafisa B, Anders E, Marcus H, Ken B-A, <br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>22. pSB1C3-B0032-BFP<br><br><h2>Todays work</h2><br><b>Plasmid preparation*: </b><br>Lb1. reuteri CF48-pLR581<br>Lb3. reuteri DSM 20016-pVs2 <br><i>*to get more useable material and to look for different result from clones grown in a lock and a CO2-cupboard.</i><br><br><b>O/N: </b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red<br>Lb6. reuteri DSM 20016-pLUL63TsA8<br>Lb7. reuteri DSM 20016-pGFP<br>Lb14. reuteri DSM 20016-pLUL631(B?)<br>Lb12. reuteri DSM 20016-noplasmid<br>Lb8. reuteri 100-23-pGT232<br>Lb10. plantarum 256-noplasmid<br>Lb11. plantarum 36E-noplasmid<br>Lb9. faecalis JH2-2 pAMβ1 *-<br><i>*from the plate without erythromycin. </i><br><br><b>Plasmid preparation:</b><br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>22. pSB1C3-B0032-BFP<br><br><h2>Results</h2><br><b>Previous day:</b><br><b>Re-streak: </b><br>Lb4. reuteri 100-23-noplasmid: No growth. → Grow on non-antibiotic plates  <br><br><b>Today:</b><br><b>Plasmid preparation:</b><br>Lb1.2 (strain 1, clone 2) reuteri CF48-pLR 581: Non-usable concentration *<br>Lb1.4 reuteri CF48-pLR 581: 161.8 ng/µl *<br>Lb3.8 reuteri DSM 20016 pVS2: 148.5 ng/µl *<br>Lb3.10 reuteri DSM 20016 pVS2: Non-usable concentration *<br><i>*there is seemingly no correlation between growth in lock vs. CO2-cupboard and plasmid concentration. This seems strange though. Cupboard is not supposed to create an anaerobic environment → do not use cupboard</i><br><br><h2>Other experiments</h2><br><b>SET buffer preparation: </b><br><b>Lysis buffer preparation: </b></div>'
  }
  }
  else if(id == 'd201373')
  else if(id == 'd201373')

Revision as of 23:55, 27 September 2013