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Team:DTU-Denmark/Notebook/28 June 2013
From 2013.igem.org
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The days PCR where not very successful. The gel pic. looks like that we can only see supercoiled plasmid DNA which must originate from the template DNA. | The days PCR where not very successful. The gel pic. looks like that we can only see supercoiled plasmid DNA which must originate from the template DNA. | ||
| - | + | [[File:29.06.13 all PCR products with x7.jpg|thumb|left|The gel from the days PCR. Note the head and trail the band leaves behind it. It looks like supercoiled plasmid DNA]] | |
== Conclusion from today == | == Conclusion from today == | ||
We might have used the wrong polymerase and the products we have gotten from previous PCRs with PHUSION-polymerase are without any uracil insertion. | We might have used the wrong polymerase and the products we have gotten from previous PCRs with PHUSION-polymerase are without any uracil insertion. | ||
Revision as of 01:02, 1 July 2013
Contents |
208 lab
Main purposes today
Make PCR with x7-polymerase.
who were in the lab
Kristian
Procedure
Made PCRs on pZA21, GFP SF TAT, GFP SF Sec, RFP all samples where made in duplicates.
In tube 1+2, 9+10 where pZA21. In 3+4, 11+12 GFP SF TAT In 5+6, 13+14, 17+18 GFP SF Sec In 7+8, 15+16 RFP
First program was 59°C annealing and 2:00 extension time with tube: 1+2, 3+4, 5+6, 7+8. Second program was 68°C annealing and 2:00 extension time with tube: 9+10, 11+12, 13+14, 15+16. Last program was 70°C annealing and 1:00 extension time with tube: 17+18.
Results
The days PCR where not very successful. The gel pic. looks like that we can only see supercoiled plasmid DNA which must originate from the template DNA.
Conclusion from today
We might have used the wrong polymerase and the products we have gotten from previous PCRs with PHUSION-polymerase are without any uracil insertion.
