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ds = '<div id="dairy-text"><h1>Wednesday 2013-07-03</h1> Name of participants: Lovisa P, Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A, Nils A, Alexander W, Christoffer, Thorsteinn, Magnus, Christoffer Ahlström (CA), Mikael Strandgren (MS), Viktor Blomkvist (VB), Stephanie Herman (SH), Anton Berglund (AB) <h2>Ongoing constructs:</h2> E.coli (strain D5α): 1. pSB1C3-red 7. pSB3K3-red 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 57. pSB1C3-B0034-Idi 58. pSB1C3-B0034-ispA 42. pSB4S15-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77. pSB4C15-red L. plantarum: Lb10. 256-noplasmid Lb11. 36E-noplasmid L. reuteri: Lb12. DSM 20016-noplasmid L. lactis: Lb13. MG1363-pJP059 <h2>Todays work</h2> PCR: Lb13.2.3 Lc. lactis MG1363-pJP059 (plasmid)* Lb13.3 Lc. lactis MG1363-pJP059 (colony)* Lb13.4 Lc. lactis MG1363-pJP059 (colony)* *Two PCR tubes of 13.2.3 were prepared along with one of 13.3 and one of 13.4. Lb13.2.2 lactis MG1363 pJP059 (plasmid)** Lb13.2.3 lactis MG1363 pJP059 (plasmid)** Lb13.3 lactis MG1363 pJP059 (colony)** Lb13.4 Lc. lactis MG1363 pJP059 (colony)** **Gel will be done tomorrow. Followed the PCR protocol with DMSO. 78. pEL3C18-CrtY* -*PCR of CrtZ with lower ™-temp(62 insted of 63.6 which was used last time) Digest: 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 25. pSB1C3-CrtY 27. pEL3K16-red 51. pEL3C18-red Ligation: 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 75. pEL3A15-CrtB 76. pEL3K16-CrtI 78. pEL3C18-CrtY 1. pSB1C3-red -as N/C, one that we use plus a digest that has worked for other groups 7. pSB3K3-red -as N/C, one that we use plus a digest that has worked for other groups 42. pSB4S15-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77. pSB4C15-red -*We increased the incubation time from 30 min. to 90 min. We also added T4 DNA ligase to the already ligated plasmids we used for transformation yesterday and left them to incubate over night, since we suspect incomplete ligation may be the explanation for the bad results (digests have appeared ok on gel). Thus if transformations fail again, we can use the definitely ligated plasmids to do another immediately in the morning. Transformation: 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 75. pEL3A15-CrtB 76. pEL3K16-CrtI 78. pEL3C18-CrtY 42. pSB4S15-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 66. pSB1C3-CP41 67. pSB1C3-Cp44 77 pSB4C15-red O/N: Lb10.1. plantarum 256 no plasmid Lb11.1. plantarum 36E no plasmid Lb12.1 reuteri DSM 20016 no plasmid Restreak: 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 57. pSB1C3-B0034-Idi 58. pSB1C3-B0034-ispA 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 67. pSB1C3-Cp44 Plasmid preparation: 7.pSB3K3 22.pSB1C3-B0032-BFP 42. pSB4S15-red Gelelectrophoresis: 19. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 78. pEL3C18-CrtY 25. pSB1C3-CrtY 27. pEL3K16-red 51. pEL3C18-red Lb13.2.3 Lc. lactis MG1363-pJP059* Lb13.3 Lc. lactis MG1363-pJP059* Lb13.4 Lc. lactis MG1363-pJP059* *Of first PCR. Two PCR tubes of 13.2.3 were prepared along with one of 13.3 and one of 13.4. Results Gelelectrophoresis: successful: 25. pSB1C3-CrtY 27. pEL3K16-red 51. pEL3C18-red -Digestion Worked failed: 19. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1 20. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1 21. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268 78. pEL3C18-CrtY Lb13.2.3 Lc. lactis MG1363-pJP059(plasmid)* Lb13.3 Lc. lactis MG1363-pJP059 (colony)* Lb13.4 Lc. lactis MG1363-pJP059 (colony)* -*The gel showed nothing but primer dimer. Digest: successful: 25. pSB1C3-CrtY 27. pEL3K16-red 51. pEL3C18-red Yesterdays transformation: successful: 61. pSB1C3-CP1: Growth 61. pSB1C3-CP1 10x: Some growth 62. pSB1C3-CP8: Some growth 62. pSB1C3-CP8 10x: No growth 63. pSB1C3-CP11: Some growth 63. pSB1C3-CP11 10x: No growth 64. pSB1C3-CP29: Some growth 64. pSB1C3-CP29 10x: No growth 65. pSB1C3-CP30: Some growth 65. pSB1C3-CP30 10x: No growth 66. pSB1C3-CP41: Growth 66. pSB1C3-CP41 10x: Some growth 67. pSB1C3-Cp44: No growth 67. pSB1C3-Cp44 10x: No growth 68. pSB3K3-CP1-B0032-BFP: No growth 68. pSB3K3-CP1-B0032-BFP 10x: Growth 69. pSB3K3-CP8-B0032-BFP: Growth, possibly contaminated 69. pSB3K3-CP8-B0032-BFP 10x: No growth 70. pSB3K3-CP11-B0032-BFP: Growth 70. pSB3K3-CP11-B0032-BFP 10x: Growth 71. pSB3K3-CP29-B0032-BFP: Growth, olly red colonies 71. pSB3K3-CP29-B0032-BFP 10x: ----- 72. pSB3K3-CP30-B0032-BFP: Growth 72. pSB3K3-CP30-B0032-BFP 10x: No growth 73. pSB3K3-CP41-B0032-BFP: Some growth, only red colonies 73. pSB3K3-CP41-B0032-BFP 10x: No growth 77.1 pSB4C15, clone 1: No growth 77.1 pSB4C15, clone 1 10x: No growth 77.2 pSB4C15, clone 2: Some growth 77.2 pSB4C15, clone 2 10x: No growth 23. pSB1C3-CrtB 24. pSB1C3-CrtI 27. pEL3K16-red 38. pEL3A15-red 51. pEL3C18-red failed: 81. pSB4S15-CP41: No growth 82. pSB4S15-CP29: No growth 83. pSB4S15-CP30: No growth → Redo ligation and transformation. Plasmid preparation: 7.3 pSB3K3 clone 3: 50.1 ng/µl 7. 4 pSB3K3 clone 4: 43.4 ng/µl 7. 5 pSB3K3 clone 5: 48.1 ng/µl 22.1.1 pSB1C3-B0032-BFP clone 1.1: 196.2 ng/µl 22.2.1 pSB1C3-B0032-BFP clone 2.1: 174.3 ng/µ 22.2.2 pSB1C3-B0032-BFP clone 2.2: 189.9 ng/µ 42.1 pSB4S15-red clone 1: 43.6 ng/µl 42.2 pSB4S15-red clone 2: 46.1 ng/µl <h2>Other experiments</h2> Made agarplates with chloramphenicol resistance MRS-broth preparation MRS-broth-agar preparation MRS-broth-agar plates preparation </div>';

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ds = '<div id="dairy-text"><h1>Thursday 2013-06-20</h1> Name of participants: Christoffer A, Stephanie H, Alona N, Viktor T, Viktor B, Mikael S, Anton B, Anders E, Emil M, Marcus H, Ken B-A, Lovisa P, Peter C, Malin B., Pontus D., Magnus B., Alexander V., Niclas S., Christoffer F. <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 E. coli: 1. pSB1C3-red 2. pSB3C5-red 6. pSB1K3-red 14. pET13b-zifz:4cl-PBSII:STS 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 22. pSB1C3-B0032-BFP 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 23. pSB1C3-CrtB 24. pSB1C3-CrtI 26. pEL3K15-red 27. pEL3K16-red 28. pEL3K17-red 29. pEL3K17(FRT)-red 30. pEL3C15-red 31. pEL3C16-red 32. pEL3C17-red 33. pEL3C17(FRT)-red 34. pEL3S15-red 35. pEL3S16-red 36. pEL3S17-red 37. pEL3S17(FRT)-red 38. pEL3A15-red 39. pEL3A16-red 40. pEL3A17-red 41. pEL3A17(FRT)-red <h2>Todays work</h2> Glycerol stock preparation: 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF Lb1. reuteri CF48-pLR581 Lb2. reuteri 1063-pLUL631 Lb3. reuteri DSM 20016-pVS2 Plasmid preparation: 14. pET13b-zifz:4cl-PBSII:STS 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 22. pSB1C3-B0032-BFP 26. pEL3K15-red 26. pEL3K15-red 27. pEL3K16-red 28. pEL3K17-red 29. pEL3K17(FRT)-red 30. pEL3C15-red 31. pEL3C16-red 32. pEL3C17-red 33. pEL3C17(FRT)-red 34. pEL3S15-red 35. pEL3S16-red 36. pEL3S17-red 37. pEL3S17(FRT)-red 38. pEL3A15-red 39. pEL3A16-red 40. pEL3A17-red 41. pEL3A17(FRT)-red Lb1. reuteri CF48-pLR581 Lb2. reuteri 1063-pLUL631 Lb3. reuteri DSM 20016-pVS2 O/N: Lb2. reuteri 1063-pLUL631 Lb1. reuteri CF48-pLR581 Lb3. reuteri DSM 20016-pVs2 Streaking of new strains from Stefan Roos: Lb6. reuteri DSM 20016-pLUL63TsA8 Lb7. reuteri DSM 20016-pGFP Lb12. reuteri DSM 20016-noplasmid * Lb14. reuteri DSM 20016-pLUL631(B?) Lb4. reuteri 100-23-noplasmid Lb8. reuteri 100-23-pGT232 * Lb5. plantarum 256-rifR-pAMβ1 Lb10. plantarum 256-noplasmid * Lb11. plantarum 36E-noplasmid * Lb9. faecalis JH2-2 pAMβ1 ** *both on burned broth without glucose and CaCl2 and regular one. **re-streaked on erythromycin and on antibiotic-free plates. Transformation: 23. pSB1C3-CrtB 24. pSB1C3-CrtI Restreaks: 1. pSB1C3-red 2. pSB3C5-red 6. pSB1K3-red <h2>Results</h2> Previous day: Lb13. Lc. lactis MG1363-pJP059 : No growth those strain. → Try another medium (e.g. M17) Today: Plasmid preparation: 19.1: 50.5 ng/µl 19.2: 33.6 ng/µl 19.3: 45.2 ng/µl 19.4: 36.5 ng/µl 20.1: 28.9 ng/µl 20.2: 29.6 ng/µl 20.3: 41.8 ng/µl 20.4: 35.8 ng/µl 21.1: 47.7 ng/µl 21.2: 23.2 ng/µl 21.3: 38.8 ng/µl 21.4: 48.9 ng/µl Lb1. reuteri CF48-pLR 581 clone 1: 74.8 ng/µl Lb1. reuteri CF48-pLR 581 clone 2: Non-usable concentration → try again. Lb1. reuteri CF48-pLR 581 clone 3: 77.5 ng/µl Lb1. reuteri CF48-pLR 581 clone 4: Non-usable concentration → try again. Lb2. reuteri 1063-pLUL 631 clone 1: 70.3 ng/µl Lb2. reuteri 1063-pLUL 631 clone 2: 39.4 ng/µl Lb2. reuteri 1063-pLUL 631 clone 3: 72.4 ng/µl Lb2. reuteri 1063-pLUL 631 clone 6: 61.0 ng/µl Lb3. reuteri DSM 20016 pVS2 clone 1: 231.7 ng/µl Lb3. reuteri DSM 20016 pVS2 clone 2: Non-usable concentration → try again. Lb3. reuteri DSM 20016 pVS2 clone 3: Non-usable concentration → try again. Lb3. reuteri DSM 20016 pVS2 clone 4: 86.3 ng/µl <h2>Other experiments</h2> Antibiotic-free MRS-broth plates preparation: SET-buffer and Lysis-buffer preparation: MRS-broth agar preparation: Preparation of plates with and without antibiotic: </div>'

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ds = '<div id="dairy-text"><h1>Wednesday 2013-06-19</h1> Name of participants: Emil M, Marcus H, Ken B-A, Sabri J., Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Jens I, Viktor T, Viktor B, Mikael S, Anton B, Malin B, Pontus D, Magnus B, Alexander W, Thorsteinn O, Niclas S, Nils A <h2>Ongoing constructs:</h2> L. reuteri: 2. 1063-pLUL631 1. CF48-pLR581 3. DSM20016-pVs2 Lactoccocus lactis: 13. MG1363-pJP059 E. coli: 1. pSB1C3-red 2. pSB3C5-red 3. pSB3T5-red 4. pSB1A3-red 6. pSB1K3-red 7. pSB3K3-red 12. pSB4K5-red 14. pET13b-zifz:4cl-PBSII:STS 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 22. pSB1C3-B0032-BFP 23. pSB1C3-CrtB 24. pSB1C3-CrtI 25. pSB1C3-CrtY 26. pEL3K15-red 27. pEL3K16-red 28. pEL3K17-red 29. pEL3K17(FRT)-red 30. pEL3C15-red 31. pEL3C16-red 32. pEL3C17-red 33. pEL3C17(FRT)-red 34. pEL3S15-red 35. pEL3S16-red 36. pEL3S17-red 37. pEL3S17(FRT)-red 38. pEL3A15-red 39. pEL3A16-red 40. pEL3A17-red 41. pEL3A17(FRT)-red <h2>Todays work</h2> Re-streak: 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 22. pSB1C3-B0032-BFP Lb1. reuteri CF48-pLR581 Lb2. reuteri 1063-pLUL631 Lb3. reuteri DSM20016-pVs2 Spreading: Lb13. Lc. lactis MG1363-pJP059 O/N: 14. pET13b-zifz:4cl-PBSII:STS 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 26. pEL3K15-red 27. pEL3K16-red 28. pEL3K17-red 29. pEL3K17(FRT)-red 30. pEL3C15-red 31. pEL3C16-red 32. pEL3C17-red 33. pEL3C17(FRT)-red 34. pEL3S15-red 35. pEL3S16-red 36. pEL3S17-red 37. pEL3S17(FRT)-red 38. pEL3A15-red 39. pEL3A16-red 40. pEL3A17-red 41. pEL3A17(FRT)-red Lb1. reuteri CF48-pLR581 Lb2. reuteri 1063-pLUL631 Lb3. reuteri DSM20016-pVs2 Transformation: 1. pSB1C3-red 2. pSB3C5-red 3. pSB3T5-red 4. pSB1A3-red 6. pSB1K3-red 7. pSB3K3-red 12. pSB4K5-red 23. pSB1C3-CrtB 24. pSB1C3-CrtI 25. pSB1C3-CrtY <h2>Results</h2> Previous day: Spreading: Lb13. Lc. lactis MG1363-pJP059 : No growth. → Try growing with less oxygen. Transformation 1. pSB1C3-red: Successful 2. pSB3C5-red: Successful 3. pSB3T5-red: fail 4. pSB1A3-red: fail 6. pSB1K3-red: Successful 7. pSB3K3-red: fail 12. pSB4K5-red: fail Restreak: 16. pSB1C3-TAL_m: Successful 17. pSB1C3-4Cl_m: Successful 18. pSB1C3-CHS_m: Successful 22. pSB1C3-B0032-BFP: Successful Today: O/N: 19. pSB4C5-CrtE~I /w RBS, Linker & ZF: Growth 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: Growth 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF: Growth Transformation: 23. pSB1C3-CrtB: fail 24. pSB1C3-CrtI: fail 25. pSB1C3-CrtY: fail <h2>Other experiments</h2> LB-agar plates CaCl2-solution</div>'

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   {
    ds = '<div id="dairy-text"><h1>Wednesday 2013-07-03</h1><br><b>Name of participants:</b> Lovisa P, Kristoffer L, Emil M, Marcus H, Karl H, Peter C, Ken B.-A,  Nils A, Alexander W, Christoffer, Thorsteinn, Magnus, Christoffer Ahlström (CA), Mikael Strandgren (MS), Viktor Blomkvist (VB), Stephanie Herman (SH), Anton Berglund (AB)<br><br><h2>Ongoing constructs:</h2><br><b>E.coli (strain D5α):</b><br>1. pSB1C3-red<br>7. pSB3K3-red<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>57. pSB1C3-B0034-Idi<br>58. pSB1C3-B0034-ispA<br>42. pSB4S15-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red<br><br><b>L. plantarum:</b><br>Lb10. 256-noplasmid<br>Lb11. 36E-noplasmid<br><br><br><b>L. reuteri:</b><br>Lb12. DSM 20016-noplasmid<br><br><b>L. lactis:</b><br>Lb13. MG1363-pJP059<br><br><h2>Todays work</h2><br><b>PCR:</b><br>Lb13.2.3 Lc. lactis MG1363-pJP059 (plasmid)*<br>Lb13.3 Lc. lactis MG1363-pJP059	  (colony)*<br>Lb13.4 Lc. lactis MG1363-pJP059	  (colony)*<br><i>*Two PCR tubes of 13.2.3 were prepared along with one of 13.3 and one of 13.4.</i><br>Lb13.2.2 lactis MG1363 pJP059	(plasmid)**<br>Lb13.2.3 lactis MG1363 pJP059	(plasmid)**<br>Lb13.3 lactis MG1363 pJP059		(colony)**<br>Lb13.4 Lc. lactis MG1363 pJP059	(colony)**<br><i>**Gel will be done tomorrow. Followed the PCR protocol with DMSO.</i><br>78. pEL3C18-CrtY*<br><i>-*PCR of CrtZ with lower ™-temp(62 insted of 63.6 which was used last time)</i><br><br>Digest:<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>51. pEL3C18-red<br><br><b>Ligation:</b><br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>75. pEL3A15-CrtB<br>76. pEL3K16-CrtI<br>78. pEL3C18-CrtY<br>1. pSB1C3-red <i>-as N/C, one that we use plus a digest that has worked for other groups</i><br>7. pSB3K3-red <i>-as N/C, one that we use plus a digest that has worked for other groups</i><br>42. pSB4S15-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77. pSB4C15-red<br><i>-*We increased the incubation time from 30 min. to 90 min. We also added T4 DNA ligase to the already ligated plasmids we used for transformation yesterday and left them to incubate over night, since we suspect incomplete ligation may be the explanation for the bad results (digests have appeared ok on gel). Thus if transformations fail again, we can use the definitely ligated plasmids to do another immediately in the morning.</i><br><br><b>Transformation:</b><br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>75. pEL3A15-CrtB<br>76. pEL3K16-CrtI<br>78. pEL3C18-CrtY<br>42. pSB4S15-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>77 pSB4C15-red<br><br><b>O/N:</b><br>Lb10.1. plantarum 256 no plasmid<br>Lb11.1. plantarum 36E no plasmid <br>Lb12.1 reuteri DSM 20016 no plasmid<br><br><b>Restreak:</b><br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>57. pSB1C3-B0034-Idi<br>58. pSB1C3-B0034-ispA<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>67. pSB1C3-Cp44<br><br><b>Plasmid preparation:</b><br>7.pSB3K3<br>22.pSB1C3-B0032-BFP<br>42. pSB4S15-red<br><br><b>Gelelectrophoresis:</b><br>19. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>78. pEL3C18-CrtY<br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>51. pEL3C18-red<br>Lb13.2.3 Lc. lactis MG1363-pJP059*<br>Lb13.3 Lc. lactis MG1363-pJP059*<br>Lb13.4 Lc. lactis MG1363-pJP059*<br><i>*Of first PCR. Two PCR tubes of 13.2.3 were prepared along with one of 13.3 and one of 13.4.</i><br><br><b>Results</b><br><b>Gelelectrophoresis:</b><br><b>successful:</b><br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>51. pEL3C18-red<br><i>-Digestion Worked</i><br><br><b>failed:</b><br>19. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-HivC-Linker-CrtO-B0034-CrtZ-Linker-Gli1<br>20. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268-B0034-PBSII-Linker-CrtY-B0034-CrtZ-Linker-Gli1<br>21. pSB4C5-pBAD/AraC-B0034-Jazz-Linker-CrtE-B0034-Blues-Linker-CrtB-B0034-CrtI-Linker-Zif268<br>78. pEL3C18-CrtY<br>Lb13.2.3 Lc. lactis MG1363-pJP059(plasmid)*<br>Lb13.3 Lc. lactis MG1363-pJP059	(colony)*<br>Lb13.4 Lc. lactis MG1363-pJP059	(colony)*<br><i>-*The gel showed nothing but primer dimer.</i><br><br><b>Digest:</b><br><b>successful:</b><br>25. pSB1C3-CrtY<br>27. pEL3K16-red<br>51. pEL3C18-red<br><br><b>Yesterdays transformation:</b><br><b>successful:</b><br>61. pSB1C3-CP1: Growth<br>61. pSB1C3-CP1 10x: Some growth<br>62. pSB1C3-CP8: Some growth<br>62. pSB1C3-CP8 10x: No growth<br>63. pSB1C3-CP11: Some growth<br>63. pSB1C3-CP11 10x: No growth<br>64. pSB1C3-CP29: Some growth<br>64. pSB1C3-CP29 10x: No growth<br>65. pSB1C3-CP30: Some growth<br>65. pSB1C3-CP30 10x: No growth<br>66. pSB1C3-CP41: Growth<br>66. pSB1C3-CP41 10x: Some growth<br>67. pSB1C3-Cp44: No growth<br>67. pSB1C3-Cp44 10x: No growth<br>68. pSB3K3-CP1-B0032-BFP: No growth<br>68. pSB3K3-CP1-B0032-BFP 10x: Growth<br>69. pSB3K3-CP8-B0032-BFP: Growth, possibly contaminated<br>69. pSB3K3-CP8-B0032-BFP 10x: No growth<br>70. pSB3K3-CP11-B0032-BFP: Growth<br>70. pSB3K3-CP11-B0032-BFP 10x:  Growth<br>71. pSB3K3-CP29-B0032-BFP: Growth, olly red colonies<br>71. pSB3K3-CP29-B0032-BFP 10x: -----<br>72. pSB3K3-CP30-B0032-BFP: Growth<br>72. pSB3K3-CP30-B0032-BFP 10x: No growth<br>73. pSB3K3-CP41-B0032-BFP: Some growth, only red colonies<br>73. pSB3K3-CP41-B0032-BFP 10x: No growth<br>77.1 pSB4C15, clone 1: No growth<br>77.1 pSB4C15, clone 1 10x: No growth<br>77.2 pSB4C15, clone 2: Some  growth<br>77.2 pSB4C15, clone 2 10x: No growth<br>23. pSB1C3-CrtB<br>24. pSB1C3-CrtI<br>27. pEL3K16-red<br>38. pEL3A15-red<br>51. pEL3C18-red<br><br><b>failed:</b><br>81. pSB4S15-CP41: No growth<br>82. pSB4S15-CP29: No growth<br>83. pSB4S15-CP30: No growth<br><i>→ Redo ligation and transformation.</i><br><br><b>Plasmid preparation:</b><br>7.3  pSB3K3 clone 3: 50.1 ng/µl<br>7. 4 pSB3K3 clone 4: 43.4 ng/µl<br>7. 5 pSB3K3 clone 5: 48.1 ng/µl<br>22.1.1 pSB1C3-B0032-BFP clone 1.1: 196.2 ng/µl<br>22.2.1 pSB1C3-B0032-BFP clone 2.1: 174.3 ng/µ<br>22.2.2 pSB1C3-B0032-BFP clone 2.2: 189.9 ng/µ<br>42.1 pSB4S15-red clone 1: 43.6 ng/µl<br>42.2 pSB4S15-red clone 2: 46.1 ng/µl<br><br><h2>Other experiments</h2><br>Made agarplates with chloramphenicol resistance<br>MRS-broth preparation<br>MRS-broth-agar preparation <br>MRS-broth-agar plates preparation </div>';
+ }
+ else if(id == 'd2013620')
+ {
+	ds = '<div id="dairy-text"><h1>Thursday 2013-06-20</h1><br><b>Name of participants: </b>Christoffer A, Stephanie H, Alona N, Viktor T, Viktor B, Mikael S, Anton B, Anders E, Emil M, Marcus H, Ken B-A, Lovisa P, Peter C, Malin B., Pontus D., Magnus B., Alexander V., Niclas S., Christoffer F.<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>22. pSB1C3-B0032-BFP<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>23. pSB1C3-CrtB<br>24. pSB1C3-CrtI<br>26. pEL3K15-red<br>27. pEL3K16-red<br>28. pEL3K17-red<br>29. pEL3K17(FRT)-red<br>30. pEL3C15-red<br>31. pEL3C16-red<br>32. pEL3C17-red<br>33. pEL3C17(FRT)-red<br>34. pEL3S15-red<br>35. pEL3S16-red<br>36. pEL3S17-red<br>37. pEL3S17(FRT)-red<br>38. pEL3A15-red<br>39. pEL3A16-red<br>40. pEL3A17-red<br>41. pEL3A17(FRT)-red<br><br><br><h2>Todays work</h2><br><b>Glycerol stock preparation: </b><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>Lb1. reuteri CF48-pLR581<br>Lb2. reuteri 1063-pLUL631<br>Lb3. reuteri DSM 20016-pVS2 <br><br><b>Plasmid preparation:</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>22. pSB1C3-B0032-BFP<br>26. pEL3K15-red<br>26. pEL3K15-red<br>27. pEL3K16-red<br>28. pEL3K17-red<br>29. pEL3K17(FRT)-red<br>30. pEL3C15-red<br>31. pEL3C16-red<br>32. pEL3C17-red<br>33. pEL3C17(FRT)-red<br>34. pEL3S15-red<br>35. pEL3S16-red<br>36. pEL3S17-red<br>37. pEL3S17(FRT)-red<br>38. pEL3A15-red<br>39. pEL3A16-red<br>40. pEL3A17-red<br>41. pEL3A17(FRT)-red<br>Lb1. reuteri CF48-pLR581<br>Lb2. reuteri 1063-pLUL631<br>Lb3. reuteri DSM 20016-pVS2 <br><br><b>O/N: </b><br>Lb2. reuteri 1063-pLUL631<br>Lb1. reuteri CF48-pLR581<br>Lb3. reuteri DSM 20016-pVs2 <br><br><b>Streaking of new strains from Stefan Roos: </b><br>Lb6. reuteri DSM 20016-pLUL63TsA8<br>Lb7. reuteri DSM 20016-pGFP<br>Lb12. reuteri DSM 20016-noplasmid *<br>Lb14. reuteri DSM 20016-pLUL631(B?)<br>Lb4. reuteri 100-23-noplasmid<br>Lb8. reuteri 100-23-pGT232 *<br>Lb5. plantarum 256-rifR-pAMβ1<br>Lb10. plantarum 256-noplasmid *<br>Lb11. plantarum 36E-noplasmid *<br>Lb9. faecalis JH2-2 pAMβ1 **<br><i>*both on burned broth without glucose and CaCl2 and regular one.</i><br><i>**re-streaked on erythromycin and on antibiotic-free plates.</i><br><br><b>Transformation:</b><br>23. pSB1C3-CrtB<br>24. pSB1C3-CrtI<br><br><b>Restreaks:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red<br><br><h2>Results</h2><br><b>Previous day:</b><br>Lb13. Lc. lactis MG1363-pJP059 : No growth those strain. → Try another medium (e.g. M17)   <br><br><b>Today:</b><br><b>Plasmid preparation: </b><br>19.1: 50.5 ng/µl  <br>19.2: 33.6 ng/µl<br>19.3: 45.2 ng/µl<br>19.4: 36.5 ng/µl<br>20.1: 28.9 ng/µl<br>20.2: 29.6 ng/µl<br>20.3: 41.8 ng/µl<br>20.4: 35.8 ng/µl<br>21.1: 47.7 ng/µl<br>21.2: 23.2 ng/µl<br>21.3: 38.8 ng/µl<br>21.4: 48.9 ng/µl<br>Lb1. reuteri CF48-pLR 581 clone 1: 74.8 ng/µl<br>Lb1. reuteri CF48-pLR 581 clone 2: Non-usable concentration → try again.<br>Lb1. reuteri CF48-pLR 581 clone 3: 77.5 ng/µl<br>Lb1. reuteri CF48-pLR 581 clone 4: Non-usable concentration → try again.<br>Lb2. reuteri 1063-pLUL 631 clone 1: 70.3 ng/µl<br>Lb2. reuteri 1063-pLUL 631 clone 2: 39.4 ng/µl<br>Lb2. reuteri 1063-pLUL 631 clone 3: 72.4 ng/µl<br>Lb2. reuteri 1063-pLUL 631 clone 6: 61.0 ng/µl<br>Lb3. reuteri DSM 20016 pVS2 clone 1: 231.7 ng/µl<br>Lb3. reuteri DSM 20016 pVS2 clone 2: Non-usable concentration → try again.<br>Lb3. reuteri DSM 20016 pVS2 clone 3: Non-usable concentration → try again.<br>Lb3. reuteri DSM 20016 pVS2 clone 4: 86.3 ng/µl<br><br><br><h2>Other experiments</h2><br><b>Antibiotic-free MRS-broth plates preparation: </b><br><b>SET-buffer and Lysis-buffer preparation: </b><br><b>MRS-broth agar preparation: </b><br><b>Preparation of plates with and without antibiotic:</b> </div>'
+ }
+   else if(id == 'd2013619')
+ {
+	ds = '<div id="dairy-text"><h1>Wednesday 2013-06-19</h1><br><br><b>Name of participants: </b>Emil M, Marcus H, Ken B-A, Sabri J., Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Jens I, Viktor T, Viktor B, Mikael S, Anton B, Malin B, Pontus D, Magnus B, Alexander W, Thorsteinn O, Niclas S, Nils A<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>2. 1063-pLUL631<br>1. CF48-pLR581<br>3. DSM20016-pVs2<br><br><b>Lactoccocus lactis:</b><br>13. MG1363-pJP059<br><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>22. pSB1C3-B0032-BFP<br>23. pSB1C3-CrtB<br>24. pSB1C3-CrtI<br>25. pSB1C3-CrtY<br>26. pEL3K15-red<br>27. pEL3K16-red<br>28. pEL3K17-red<br>29. pEL3K17(FRT)-red<br>30. pEL3C15-red<br>31. pEL3C16-red<br>32. pEL3C17-red<br>33. pEL3C17(FRT)-red<br>34. pEL3S15-red<br>35. pEL3S16-red<br>36. pEL3S17-red<br>37. pEL3S17(FRT)-red<br>38. pEL3A15-red<br>39. pEL3A16-red<br>40. pEL3A17-red<br>41. pEL3A17(FRT)-red  <br><br><h2>Todays work</h2><br><b>Re-streak:</b><br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>22. pSB1C3-B0032-BFP<br>Lb1. reuteri CF48-pLR581<br>Lb2. reuteri 1063-pLUL631<br>Lb3. reuteri DSM20016-pVs2<br><br><b>Spreading:</b> <br>Lb13. Lc. lactis MG1363-pJP059 <br><br><b>O/N:</b> <br>14. pET13b-zifz:4cl-PBSII:STS<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>26. pEL3K15-red<br>27. pEL3K16-red<br>28. pEL3K17-red<br>29. pEL3K17(FRT)-red<br>30. pEL3C15-red<br>31. pEL3C16-red<br>32. pEL3C17-red<br>33. pEL3C17(FRT)-red<br>34. pEL3S15-red<br>35. pEL3S16-red<br>36. pEL3S17-red<br>37. pEL3S17(FRT)-red<br>38. pEL3A15-red<br>39. pEL3A16-red<br>40. pEL3A17-red<br>41. pEL3A17(FRT)-red<br>Lb1. reuteri CF48-pLR581<br>Lb2. reuteri 1063-pLUL631<br>Lb3. reuteri DSM20016-pVs2<br><br><b>Transformation:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>23. pSB1C3-CrtB<br>24. pSB1C3-CrtI<br>25. pSB1C3-CrtY<br><br><h2>Results</h2><br><b>Previous day: </b><br><b>Spreading:</b><br>Lb13. Lc. lactis MG1363-pJP059 : No growth. → Try growing with less oxygen. <br><br><b>Transformation</b><br>1. pSB1C3-red: Successful<br>2. pSB3C5-red: Successful<br>3. pSB3T5-red: fail<br>4. pSB1A3-red: fail<br>6. pSB1K3-red: Successful<br>7. pSB3K3-red: fail<br>12. pSB4K5-red: fail<br><br><b>Restreak:</b><br>16. pSB1C3-TAL_m: Successful<br>17. pSB1C3-4Cl_m: Successful<br>18. pSB1C3-CHS_m: Successful<br>22. pSB1C3-B0032-BFP: Successful<br><br><b>Today:</b><br><b>O/N: </b><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF: Growth<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: Growth<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF: Growth<br><br><b>Transformation:</b><br>23. pSB1C3-CrtB: fail<br>24. pSB1C3-CrtI: fail<br>25. pSB1C3-CrtY: fail<br><br><h2>Other experiments</h2><br><b>LB-agar plates </b><br><b>CaCl2-solution</b></div>'
   }
   else if(id == 'd201372')

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Revision as of 00:48, 28 September 2013