Team:BYU Provo/Notebook/Phage Purification/Fallexp/Period3/Dailylog

From 2013.igem.org

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<font size="4"> '''9/16/13''' </font>
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<font size="4"> '''10/21/13''' </font>
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- We ran three different gradients for the small phage team today.  We ran one of the wild type, one targeting large phage, and one targeting small phage.
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-Today we ran PCR on T4 wild type and mutant phage to determine the sequence differences in the capsid proteins.
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:: - [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.16CsClGradient|9.16 CsCl Gradient]]
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:: - [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/10.21T4PCR|10.21 T4 PCR]]
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<font size="4"> '''9/18/13''' </font>
 
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- We focussed on completing the wiki and finishing the videos for our team collaboration.  The T7 phage from the previous gradient were spot tested by the small phage team and we will have the results to that next class.
 
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<font size="4"> '''9/20/13''' </font>
 
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- The T7 group spot tested the aliquots and found concentrations to the -4 in almost every aliquot. Hopefully, with the knowledge that wild type bands at 1.3 we will be able to isolate mutated phage. Today we worked extensively on the Wiki as well and began math modeling for our results.
 
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<font size="4"> '''9/23/13''' </font>
 
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- We worked on the wiki and math modeling.
 
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<font size="4"> '''9/25/13''' </font>
 
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- We focussed on completing our final projects for the presentation next week.
 
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<font size="4"> '''9/27/13''' </font>
 
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- We worked on finalizing our wiki today before the wiki freeze.
 
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Revision as of 21:06, 21 October 2013


Phage Purification September - October Notebook: September 15 - September 30 Daily Log



Phage Purification
March-April
May-June
July-August
September-October



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10/21/13

-Today we ran PCR on T4 wild type and mutant phage to determine the sequence differences in the capsid proteins.

- 10.21 T4 PCR

AB DL AC



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