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Team:British Columbia/Notebook/Protocols
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===[[Team:British_Columbia/Notebook/Protocols/Biobrickdigest|BioBrick Restriction Digest]]=== | ===[[Team:British_Columbia/Notebook/Protocols/Biobrickdigest|BioBrick Restriction Digest]]=== | ||
Restriction digest with BioBrick plasmid an inserts | Restriction digest with BioBrick plasmid an inserts | ||
| + | |||
| + | ===[[Team:British_Columbia/Notebook/Protocols/ligation|BioBrick Ligation]]=== | ||
| + | Ligate Biobrick insert to vector | ||
===[[Team:British_Columbia/Notebook/Protocols/RepeatSpacerAssembly |CRISPR Repeat-Spacer Array Assembly]]=== | ===[[Team:British_Columbia/Notebook/Protocols/RepeatSpacerAssembly |CRISPR Repeat-Spacer Array Assembly]]=== | ||
Revision as of 00:42, 28 September 2013
iGEM Home
Protocols
PCR
Instruction for colony PCR and regular PCR
Gel Verification
Make a gel and conduct gel elecrophoresis
BioBrick Restriction Digest
Restriction digest with BioBrick plasmid an inserts
BioBrick Ligation
Ligate Biobrick insert to vector
CRISPR Repeat-Spacer Array Assembly
Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides
Assembling Parts from Oligonucleotides
Assemble short parts from annealed oligonucleotides
(Multi) Site Directed Mutagenesis
Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.
Oligonucleotide Phosphorylation
Phosphorylate oligonucleotides prior to ligation
Electrocompetent Cell Production
Gas chromatography-mass spectrometry for biosynthetic product detection
GC-MS Protocol for assaying biosynthetic product production.
Make Combinatorial Part Libraries
Make combinatorial part libraries from compatible parts
