Team:NYMU-Taipei

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Revision as of 00:40, 28 September 2013

National Yang Ming University
Taipei, Taiwan

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Overview of 2013 NYMU iGEM Team Project

To save bees from Nosema ceranae infection, the culprit of colony collapse disorder (CCD), NYMU-Taipei iGEM team has created the so-called Bee. coli from Escherichia coli MG1655, which naturally resides in bees. The Bee. coli is designed to work successively as follows: (1) Bee. coli continuously secretes mannosidase to inhibit the sprouting of N. ceranae spores. (2) if the bee is infected with N. ceranae, the fungus-killing-circuit with a positive feedback design will be turned on to wipe out N. ceranae. (3) if these designer weapons should fail to conquer N. ceranae, a bee-suicide-operon will be activated to kill the infected bees but save their companions.

Besides, a light-inducible lysis system is created to ensure our Bee. coil only lives inside of the bee. In addition, we have used encapsulation as the method to send our Bee. coli into the bee. Since the capsule will only dissolve in a bee’s gut, our Bee. coli will not wantonly spread out.

Achievements

1. We have documented and sent 23 new standard BioBrick Parts or devices to part registry.

2. We have done the experiments to prove that at least 16 parts or devices that we sent really work as expected. The results are shown in each parts’ registry page.

3. We have demonstrated a convenient and safe way to send genetic modified E. coli into other organism and prove to be effective in bee in vivo.

4. We have designed a light-induced lysis system to make sure genetic modified E. coli will not spread through the environment.

5. We improve the function of an existing BioBrick Part AhpC promoter K362001 by point mutation and enzyme splicing.

6. We have constructed a T7 polymerase mediated positive feedback circuit.

7. We help NTU-Taida characterized their BioBrick devices, the ACE (BBa_K1157020) and CEA (BBa_K1157021).

8. We have made some videos of doing experiments. They are the way we teach students how to conduct wet lab experiments.

9. We have gone to bee farms and help the beekeepers to check if their bees were infected by Nosema ceranea.

10. We created primers to identified Nosema ceranea. This provides a fast and precise way to find out Nosema ceranea besides barely using microscope.

Revision as of 00:38, 28 September 2013 (view source) Lawrence19993 (Talk \| contribs) ← Older edit		Revision as of 00:40, 28 September 2013 (view source) Lawrence19993 (Talk \| contribs) Newer edit →
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	[https://2013.igem.org/Team:NYMU-Taipei/HumanPractice/HumPracticalApp 9. We have gone to bee farms and help the beekeepers to check if their bees were infected by Nosema ceranea.]		[https://2013.igem.org/Team:NYMU-Taipei/HumanPractice/HumPracticalApp 9. We have gone to bee farms and help the beekeepers to check if their bees were infected by Nosema ceranea.]
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	[https://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Id-Nosema 10. We created primers to identified Nosema ceranea. This provides a fast and precise way to find out Nosema ceranea besides barely using microscope.]		[https://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Id-Nosema 10. We created primers to identified Nosema ceranea. This provides a fast and precise way to find out Nosema ceranea besides barely using microscope.]