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ds = '<div id="dairy-text"><h1>Thursday 2013-06-20</h1> Name of participants: Christoffer A, Stephanie H, Alona N, Viktor T, Viktor B, Mikael S (the best), Anton B, Anders E, Emil M, Marcus H, Ken B-A, Lovisa P, Peter C, Malin B., Pontus D., Magnus B., Alexander W., Niclas S., Christoffer F. <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 E. coli: 1. pSB1C3-red 2. pSB3C5-red 6. pSB1K3-red 14. pET13b-zifz:4cl-PBSII:STS 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 22. pSB1C3-B0032-BFP 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 23. pSB1C3-CrtB 24. pSB1C3-CrtI 26. pEL3K15-red 27. pEL3K16-red 28. pEL3K17-red 29. pEL3K17(FRT)-red 30. pEL3C15-red 31. pEL3C16-red 32. pEL3C17-red 33. pEL3C17(FRT)-red 34. pEL3S15-red 35. pEL3S16-red 36. pEL3S17-red 37. pEL3S17(FRT)-red 38. pEL3A15-red 39. pEL3A16-red 40. pEL3A17-red 41. pEL3A17(FRT)-red <h2>Todays work:</h2> Glycerol stock preparation: 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF Lb1. reuteri CF48-pLR581 Lb2. reuteri 1063-pLUL631 Lb3. reuteri DSM 20016-pVS2 Plasmid preparation: 14. pET13b-zifz:4cl-PBSII:STS 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 22. pSB1C3-B0032-BFP 26. pEL3K15-red 26. pEL3K15-red 27. pEL3K16-red 28. pEL3K17-red 29. pEL3K17(FRT)-red 30. pEL3C15-red 31. pEL3C16-red 32. pEL3C17-red 33. pEL3C17(FRT)-red 34. pEL3S15-red 35. pEL3S16-red 36. pEL3S17-red 37. pEL3S17(FRT)-red 38. pEL3A15-red 39. pEL3A16-red 40. pEL3A17-red 41. pEL3A17(FRT)-red Lb1. reuteri CF48-pLR581 Lb2. reuteri 1063-pLUL631 Lb3. reuteri DSM 20016-pVS2 O/N: Lb2. reuteri 1063-pLUL631 Lb1. reuteri CF48-pLR581 Lb3. reuteri DSM 20016-pVs2 Streaking of new strains from Stefan Roos: Lb6. reuteri DSM 20016-pLUL63TsA8 Lb7. reuteri DSM 20016-pGFP Lb12. reuteri DSM 20016-noplasmid * Lb14. reuteri DSM 20016-pLUL631(B?) Lb4. reuteri 100-23-noplasmid Lb8. reuteri 100-23-pGT232 * Lb5. plantarum 256-rifR-pAMβ1 Lb10. plantarum 256-noplasmid * Lb11. plantarum 36E-noplasmid * Lb9. faecalis JH2-2 pAMβ1 ** *both on burned broth without glucose and CaCl2 and regular one. **re-streaked on erythromycin and on antibiotic-free plates. Transformation: 23. pSB1C3-CrtB 24. pSB1C3-CrtI Restreaks: 1. pSB1C3-red 2. pSB3C5-red 6. pSB1K3-red <h2>Results</h2> Previous day: Lb13. Lc. lactis MG1363-pJP059 : No growth those strain. → Try another medium (e.g. M17) Today: Plasmid preparation: 19.1: 50.5 ng/µl 19.2: 33.6 ng/µl 19.3: 45.2 ng/µl 19.4: 36.5 ng/µl 20.1: 28.9 ng/µl 20.2: 29.6 ng/µl 20.3: 41.8 ng/µl 20.4: 35.8 ng/µl 21.1: 47.7 ng/µl 21.2: 23.2 ng/µl 21.3: 38.8 ng/µl 21.4: 48.9 ng/µl Lb1. reuteri CF48-pLR 581 clone 1: 74.8 ng/µl Lb1. reuteri CF48-pLR 581 clone 2: Non-usable concentration → try again. Lb1. reuteri CF48-pLR 581 clone 3: 77.5 ng/µl Lb1. reuteri CF48-pLR 581 clone 4: Non-usable concentration → try again. Lb2. reuteri 1063-pLUL 631 clone 1: 70.3 ng/µl Lb2. reuteri 1063-pLUL 631 clone 2: 39.4 ng/µl Lb2. reuteri 1063-pLUL 631 clone 3: 72.4 ng/µl Lb2. reuteri 1063-pLUL 631 clone 6: 61.0 ng/µl Lb3. reuteri DSM 20016 pVS2 clone 1: 231.7 ng/µl Lb3. reuteri DSM 20016 pVS2 clone 2: Non-usable concentration → try again. Lb3. reuteri DSM 20016 pVS2 clone 3: Non-usable concentration → try again. Lb3. reuteri DSM 20016 pVS2 clone 4: 86.3 ng/µl <h2>Other experiments</h2> Antibiotic-free MRS-broth plates preparation: SET-buffer and Lysis-buffer preparation: MRS-broth agar preparation: Preparation of plates with and without antibiotic: </div>'

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ds = '<div id="dairy-text"><h1>Thursday 2013-06-20</h1> Name of participants: Christoffer A, Stephanie H, Alona N, Viktor T, Viktor B, Mikael S (the best, hi to anyone reading), Anton B, Anders E, Emil M, Marcus H, Ken B-A, Lovisa P, Peter C, Malin B., Pontus D., Magnus B., Alexander W., Niclas S., Christoffer F. <h2>Ongoing constructs:</h2> L. reuteri: 1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP 8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 10. 256-noplasmid 11. 36E-noplasmid E. faecalis: 9. JH2-2 pAMβ1 E. coli: 1. pSB1C3-red 2. pSB3C5-red 6. pSB1K3-red 14. pET13b-zifz:4cl-PBSII:STS 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 22. pSB1C3-B0032-BFP 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 23. pSB1C3-CrtB 24. pSB1C3-CrtI 26. pEL3K15-red 27. pEL3K16-red 28. pEL3K17-red 29. pEL3K17(FRT)-red 30. pEL3C15-red 31. pEL3C16-red 32. pEL3C17-red 33. pEL3C17(FRT)-red 34. pEL3S15-red 35. pEL3S16-red 36. pEL3S17-red 37. pEL3S17(FRT)-red 38. pEL3A15-red 39. pEL3A16-red 40. pEL3A17-red 41. pEL3A17(FRT)-red <h2>Todays work:</h2> Glycerol stock preparation: 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF Lb1. reuteri CF48-pLR581 Lb2. reuteri 1063-pLUL631 Lb3. reuteri DSM 20016-pVS2 Plasmid preparation: 14. pET13b-zifz:4cl-PBSII:STS 16. pSB1C3-TAL_m 17. pSB1C3-4Cl_m 18. pSB1C3-CHS_m 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 22. pSB1C3-B0032-BFP 26. pEL3K15-red 26. pEL3K15-red 27. pEL3K16-red 28. pEL3K17-red 29. pEL3K17(FRT)-red 30. pEL3C15-red 31. pEL3C16-red 32. pEL3C17-red 33. pEL3C17(FRT)-red 34. pEL3S15-red 35. pEL3S16-red 36. pEL3S17-red 37. pEL3S17(FRT)-red 38. pEL3A15-red 39. pEL3A16-red 40. pEL3A17-red 41. pEL3A17(FRT)-red Lb1. reuteri CF48-pLR581 Lb2. reuteri 1063-pLUL631 Lb3. reuteri DSM 20016-pVS2 O/N: Lb2. reuteri 1063-pLUL631 Lb1. reuteri CF48-pLR581 Lb3. reuteri DSM 20016-pVs2 Streaking of new strains from Stefan Roos: Lb6. reuteri DSM 20016-pLUL63TsA8 Lb7. reuteri DSM 20016-pGFP Lb12. reuteri DSM 20016-noplasmid * Lb14. reuteri DSM 20016-pLUL631(B?) Lb4. reuteri 100-23-noplasmid Lb8. reuteri 100-23-pGT232 * Lb5. plantarum 256-rifR-pAMβ1 Lb10. plantarum 256-noplasmid * Lb11. plantarum 36E-noplasmid * Lb9. faecalis JH2-2 pAMβ1 ** *both on burned broth without glucose and CaCl2 and regular one. **re-streaked on erythromycin and on antibiotic-free plates. Transformation: 23. pSB1C3-CrtB 24. pSB1C3-CrtI Restreaks: 1. pSB1C3-red 2. pSB3C5-red 6. pSB1K3-red <h2>Results</h2> Previous day: Lb13. Lc. lactis MG1363-pJP059 : No growth those strain. → Try another medium (e.g. M17) Today: Plasmid preparation: 19.1: 50.5 ng/µl 19.2: 33.6 ng/µl 19.3: 45.2 ng/µl 19.4: 36.5 ng/µl 20.1: 28.9 ng/µl 20.2: 29.6 ng/µl 20.3: 41.8 ng/µl 20.4: 35.8 ng/µl 21.1: 47.7 ng/µl 21.2: 23.2 ng/µl 21.3: 38.8 ng/µl 21.4: 48.9 ng/µl Lb1. reuteri CF48-pLR 581 clone 1: 74.8 ng/µl Lb1. reuteri CF48-pLR 581 clone 2: Non-usable concentration → try again. Lb1. reuteri CF48-pLR 581 clone 3: 77.5 ng/µl Lb1. reuteri CF48-pLR 581 clone 4: Non-usable concentration → try again. Lb2. reuteri 1063-pLUL 631 clone 1: 70.3 ng/µl Lb2. reuteri 1063-pLUL 631 clone 2: 39.4 ng/µl Lb2. reuteri 1063-pLUL 631 clone 3: 72.4 ng/µl Lb2. reuteri 1063-pLUL 631 clone 6: 61.0 ng/µl Lb3. reuteri DSM 20016 pVS2 clone 1: 231.7 ng/µl Lb3. reuteri DSM 20016 pVS2 clone 2: Non-usable concentration → try again. Lb3. reuteri DSM 20016 pVS2 clone 3: Non-usable concentration → try again. Lb3. reuteri DSM 20016 pVS2 clone 4: 86.3 ng/µl <h2>Other experiments</h2> Antibiotic-free MRS-broth plates preparation: SET-buffer and Lysis-buffer preparation: MRS-broth agar preparation: Preparation of plates with and without antibiotic: </div>'

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   {
-	ds = '<div id="dairy-text"><h1>Thursday 2013-06-20</h1><br><b>Name of participants: </b>Christoffer A, Stephanie H, Alona N, Viktor T, Viktor B, Mikael S (the best), Anton B, Anders E, Emil M, Marcus H, Ken B-A, Lovisa P, Peter C, Malin B., Pontus D., Magnus B., Alexander W., Niclas S., Christoffer F.<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>22. pSB1C3-B0032-BFP<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>23. pSB1C3-CrtB<br>24. pSB1C3-CrtI<br>26. pEL3K15-red<br>27. pEL3K16-red<br>28. pEL3K17-red<br>29. pEL3K17(FRT)-red<br>30. pEL3C15-red<br>31. pEL3C16-red<br>32. pEL3C17-red<br>33. pEL3C17(FRT)-red<br>34. pEL3S15-red<br>35. pEL3S16-red<br>36. pEL3S17-red<br>37. pEL3S17(FRT)-red<br>38. pEL3A15-red<br>39. pEL3A16-red<br>40. pEL3A17-red<br>41. pEL3A17(FRT)-red<br><br><br><h2>Todays work:</h2><br><b>Glycerol stock preparation: </b><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>Lb1. reuteri CF48-pLR581<br>Lb2. reuteri 1063-pLUL631<br>Lb3. reuteri DSM 20016-pVS2 <br><br><b>Plasmid preparation:</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>22. pSB1C3-B0032-BFP<br>26. pEL3K15-red<br>26. pEL3K15-red<br>27. pEL3K16-red<br>28. pEL3K17-red<br>29. pEL3K17(FRT)-red<br>30. pEL3C15-red<br>31. pEL3C16-red<br>32. pEL3C17-red<br>33. pEL3C17(FRT)-red<br>34. pEL3S15-red<br>35. pEL3S16-red<br>36. pEL3S17-red<br>37. pEL3S17(FRT)-red<br>38. pEL3A15-red<br>39. pEL3A16-red<br>40. pEL3A17-red<br>41. pEL3A17(FRT)-red<br>Lb1. reuteri CF48-pLR581<br>Lb2. reuteri 1063-pLUL631<br>Lb3. reuteri DSM 20016-pVS2 <br><br><b>O/N: </b><br>Lb2. reuteri 1063-pLUL631<br>Lb1. reuteri CF48-pLR581<br>Lb3. reuteri DSM 20016-pVs2 <br><br><b>Streaking of new strains from Stefan Roos: </b><br>Lb6. reuteri DSM 20016-pLUL63TsA8<br>Lb7. reuteri DSM 20016-pGFP<br>Lb12. reuteri DSM 20016-noplasmid *<br>Lb14. reuteri DSM 20016-pLUL631(B?)<br>Lb4. reuteri 100-23-noplasmid<br>Lb8. reuteri 100-23-pGT232 *<br>Lb5. plantarum 256-rifR-pAMβ1<br>Lb10. plantarum 256-noplasmid *<br>Lb11. plantarum 36E-noplasmid *<br>Lb9. faecalis JH2-2 pAMβ1 **<br><i>*both on burned broth without glucose and CaCl2 and regular one.</i><br><i>**re-streaked on erythromycin and on antibiotic-free plates.</i><br><br><b>Transformation:</b><br>23. pSB1C3-CrtB<br>24. pSB1C3-CrtI<br><br><b>Restreaks:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red<br><br><h2>Results</h2><br><b>Previous day:</b><br>Lb13. Lc. lactis MG1363-pJP059 : No growth those strain. → Try another medium (e.g. M17)   <br><br><b>Today:</b><br><b>Plasmid preparation: </b><br>19.1: 50.5 ng/µl  <br>19.2: 33.6 ng/µl<br>19.3: 45.2 ng/µl<br>19.4: 36.5 ng/µl<br>20.1: 28.9 ng/µl<br>20.2: 29.6 ng/µl<br>20.3: 41.8 ng/µl<br>20.4: 35.8 ng/µl<br>21.1: 47.7 ng/µl<br>21.2: 23.2 ng/µl<br>21.3: 38.8 ng/µl<br>21.4: 48.9 ng/µl<br>Lb1. reuteri CF48-pLR 581 clone 1: 74.8 ng/µl<br>Lb1. reuteri CF48-pLR 581 clone 2: Non-usable concentration → try again.<br>Lb1. reuteri CF48-pLR 581 clone 3: 77.5 ng/µl<br>Lb1. reuteri CF48-pLR 581 clone 4: Non-usable concentration → try again.<br>Lb2. reuteri 1063-pLUL 631 clone 1: 70.3 ng/µl<br>Lb2. reuteri 1063-pLUL 631 clone 2: 39.4 ng/µl<br>Lb2. reuteri 1063-pLUL 631 clone 3: 72.4 ng/µl<br>Lb2. reuteri 1063-pLUL 631 clone 6: 61.0 ng/µl<br>Lb3. reuteri DSM 20016 pVS2 clone 1: 231.7 ng/µl<br>Lb3. reuteri DSM 20016 pVS2 clone 2: Non-usable concentration → try again.<br>Lb3. reuteri DSM 20016 pVS2 clone 3: Non-usable concentration → try again.<br>Lb3. reuteri DSM 20016 pVS2 clone 4: 86.3 ng/µl<br><br><br><h2>Other experiments</h2><br><b>Antibiotic-free MRS-broth plates preparation: </b><br><b>SET-buffer and Lysis-buffer preparation: </b><br><b>MRS-broth agar preparation: </b><br><b>Preparation of plates with and without antibiotic:</b> </div>'
+	ds = '<div id="dairy-text"><h1>Thursday 2013-06-20</h1><br><b>Name of participants: </b>Christoffer A, Stephanie H, Alona N, Viktor T, Viktor B, Mikael S (the best, hi to anyone reading), Anton B, Anders E, Emil M, Marcus H, Ken B-A, Lovisa P, Peter C, Malin B., Pontus D., Magnus B., Alexander W., Niclas S., Christoffer F.<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>10. 256-noplasmid<br>11. 36E-noplasmid<br><br><b>E. faecalis:</b><br>9. JH2-2 pAMβ1<br><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>22. pSB1C3-B0032-BFP<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>23. pSB1C3-CrtB<br>24. pSB1C3-CrtI<br>26. pEL3K15-red<br>27. pEL3K16-red<br>28. pEL3K17-red<br>29. pEL3K17(FRT)-red<br>30. pEL3C15-red<br>31. pEL3C16-red<br>32. pEL3C17-red<br>33. pEL3C17(FRT)-red<br>34. pEL3S15-red<br>35. pEL3S16-red<br>36. pEL3S17-red<br>37. pEL3S17(FRT)-red<br>38. pEL3A15-red<br>39. pEL3A16-red<br>40. pEL3A17-red<br>41. pEL3A17(FRT)-red<br><br><br><h2>Todays work:</h2><br><b>Glycerol stock preparation: </b><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>Lb1. reuteri CF48-pLR581<br>Lb2. reuteri 1063-pLUL631<br>Lb3. reuteri DSM 20016-pVS2 <br><br><b>Plasmid preparation:</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>22. pSB1C3-B0032-BFP<br>26. pEL3K15-red<br>26. pEL3K15-red<br>27. pEL3K16-red<br>28. pEL3K17-red<br>29. pEL3K17(FRT)-red<br>30. pEL3C15-red<br>31. pEL3C16-red<br>32. pEL3C17-red<br>33. pEL3C17(FRT)-red<br>34. pEL3S15-red<br>35. pEL3S16-red<br>36. pEL3S17-red<br>37. pEL3S17(FRT)-red<br>38. pEL3A15-red<br>39. pEL3A16-red<br>40. pEL3A17-red<br>41. pEL3A17(FRT)-red<br>Lb1. reuteri CF48-pLR581<br>Lb2. reuteri 1063-pLUL631<br>Lb3. reuteri DSM 20016-pVS2 <br><br><b>O/N: </b><br>Lb2. reuteri 1063-pLUL631<br>Lb1. reuteri CF48-pLR581<br>Lb3. reuteri DSM 20016-pVs2 <br><br><b>Streaking of new strains from Stefan Roos: </b><br>Lb6. reuteri DSM 20016-pLUL63TsA8<br>Lb7. reuteri DSM 20016-pGFP<br>Lb12. reuteri DSM 20016-noplasmid *<br>Lb14. reuteri DSM 20016-pLUL631(B?)<br>Lb4. reuteri 100-23-noplasmid<br>Lb8. reuteri 100-23-pGT232 *<br>Lb5. plantarum 256-rifR-pAMβ1<br>Lb10. plantarum 256-noplasmid *<br>Lb11. plantarum 36E-noplasmid *<br>Lb9. faecalis JH2-2 pAMβ1 **<br><i>*both on burned broth without glucose and CaCl2 and regular one.</i><br><i>**re-streaked on erythromycin and on antibiotic-free plates.</i><br><br><b>Transformation:</b><br>23. pSB1C3-CrtB<br>24. pSB1C3-CrtI<br><br><b>Restreaks:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>6. pSB1K3-red<br><br><h2>Results</h2><br><b>Previous day:</b><br>Lb13. Lc. lactis MG1363-pJP059 : No growth those strain. → Try another medium (e.g. M17)   <br><br><b>Today:</b><br><b>Plasmid preparation: </b><br>19.1: 50.5 ng/µl  <br>19.2: 33.6 ng/µl<br>19.3: 45.2 ng/µl<br>19.4: 36.5 ng/µl<br>20.1: 28.9 ng/µl<br>20.2: 29.6 ng/µl<br>20.3: 41.8 ng/µl<br>20.4: 35.8 ng/µl<br>21.1: 47.7 ng/µl<br>21.2: 23.2 ng/µl<br>21.3: 38.8 ng/µl<br>21.4: 48.9 ng/µl<br>Lb1. reuteri CF48-pLR 581 clone 1: 74.8 ng/µl<br>Lb1. reuteri CF48-pLR 581 clone 2: Non-usable concentration → try again.<br>Lb1. reuteri CF48-pLR 581 clone 3: 77.5 ng/µl<br>Lb1. reuteri CF48-pLR 581 clone 4: Non-usable concentration → try again.<br>Lb2. reuteri 1063-pLUL 631 clone 1: 70.3 ng/µl<br>Lb2. reuteri 1063-pLUL 631 clone 2: 39.4 ng/µl<br>Lb2. reuteri 1063-pLUL 631 clone 3: 72.4 ng/µl<br>Lb2. reuteri 1063-pLUL 631 clone 6: 61.0 ng/µl<br>Lb3. reuteri DSM 20016 pVS2 clone 1: 231.7 ng/µl<br>Lb3. reuteri DSM 20016 pVS2 clone 2: Non-usable concentration → try again.<br>Lb3. reuteri DSM 20016 pVS2 clone 3: Non-usable concentration → try again.<br>Lb3. reuteri DSM 20016 pVS2 clone 4: 86.3 ng/µl<br><br><br><h2>Other experiments</h2><br><b>Antibiotic-free MRS-broth plates preparation: </b><br><b>SET-buffer and Lysis-buffer preparation: </b><br><b>MRS-broth agar preparation: </b><br><b>Preparation of plates with and without antibiotic:</b> </div>'
   }
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