Template:Team:Uppsala/JS/notebook

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(Difference between revisions)
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  {
  {
ds = '<div id="dairy-text"><h1>Tuesday 2013-06-18</h1><br><b>Name of participants: </b>Malin B, Pontus D, Magnus B, Alexander V, Thorsteinn O, Niclas S, Nils A, Christoffer F, Kristoffer L, Emil M, Theodor L, Marcus H, Ken B.-A, Peter C, Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Jens I, Viktor T, Viktor B, Mikael S, Anton B<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>2. 1063-pLUL631<br>1. CF48-pLR581<br>3. DSM20016-pVs2<br> <br><b>Lactoccocus lactis:</b><br>13. MG1363-pJP059  <br><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>22. pSB1C3-B0032-BFP<br><br><h2>Todays work:</h2><br><b>Streaking of strains from Stefan Roos: </b><br>Lb1. reuteri CF48-pLR581<br>Lb2. reuteri 1063-pLUL631 <br>Lb3. reuteri DSM 20016-pVS2<br>Lb13. Lc. lactis MG1363-pJP059 <br><br><b>Transformation</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>22. pSB1C3-B0032-BFP<br><br><b>Restreak</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br><br><h2>Results:</h2><br><b>Previous day</b><br><b>Restreak:</b><br>14. pET13b-zifz:4cl-PBSII:STS: Growth<br><br><b>Today</b><br><b>Transformation:</b><br>1. pSB1C3-red: fail<br>2. pSB3C5-red: fail<br>3. pSB3T5-red: fail<br>4. pSB1A3-red: fail<br>6. pSB1K3-red: fail<br>7. pSB3K3-red: fail<br>12. pSB4K5-red: fail<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT: fail<br>16. pSB1C3-TAL_m: successful<br>17. pSB1C3-4Cl_m: successful<br>18. pSB1C3-CHS_m: successful<br>22. pSB1C3-B0032-BFP: successful<br><br><b>Restreak:</b><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF: Growth<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: Growth<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF: Growth<br><br><h2>Other experiments:</h2><br><b>LB-agar</b></div>'
ds = '<div id="dairy-text"><h1>Tuesday 2013-06-18</h1><br><b>Name of participants: </b>Malin B, Pontus D, Magnus B, Alexander V, Thorsteinn O, Niclas S, Nils A, Christoffer F, Kristoffer L, Emil M, Theodor L, Marcus H, Ken B.-A, Peter C, Christoffer A, Nafisa B, Stephanie H, Alona N, Anders E, Jens I, Viktor T, Viktor B, Mikael S, Anton B<br><br><h2>Ongoing constructs:</h2><br><b>L. reuteri: </b><br>2. 1063-pLUL631<br>1. CF48-pLR581<br>3. DSM20016-pVs2<br> <br><b>Lactoccocus lactis:</b><br>13. MG1363-pJP059  <br><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>22. pSB1C3-B0032-BFP<br><br><h2>Todays work:</h2><br><b>Streaking of strains from Stefan Roos: </b><br>Lb1. reuteri CF48-pLR581<br>Lb2. reuteri 1063-pLUL631 <br>Lb3. reuteri DSM 20016-pVS2<br>Lb13. Lc. lactis MG1363-pJP059 <br><br><b>Transformation</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>22. pSB1C3-B0032-BFP<br><br><b>Restreak</b><br>14. pET13b-zifz:4cl-PBSII:STS<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br><br><h2>Results:</h2><br><b>Previous day</b><br><b>Restreak:</b><br>14. pET13b-zifz:4cl-PBSII:STS: Growth<br><br><b>Today</b><br><b>Transformation:</b><br>1. pSB1C3-red: fail<br>2. pSB3C5-red: fail<br>3. pSB3T5-red: fail<br>4. pSB1A3-red: fail<br>6. pSB1K3-red: fail<br>7. pSB3K3-red: fail<br>12. pSB4K5-red: fail<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT: fail<br>16. pSB1C3-TAL_m: successful<br>17. pSB1C3-4Cl_m: successful<br>18. pSB1C3-CHS_m: successful<br>22. pSB1C3-B0032-BFP: successful<br><br><b>Restreak:</b><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF: Growth<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: Growth<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF: Growth<br><br><h2>Other experiments:</h2><br><b>LB-agar</b></div>'
-
  }  else if(id == 'd2013617')
+
  }   
 +
else if(id == 'd2013617')
  {
  {
ds = '<div id="dairy-text"><h1>Monday 2013-06-17</h1><br><b>Name of participants: </b>Kristoffer L, Emil M, Lovisa P, Theodor L, Marcus H, Ken B-A, Peter C, Christoffer A, Anton B, Nafisa B, Stephanie H, Alona N, Anders E, Malin B., Pontus D., Alexander V., Magnus B., Nils A. Thorsteinn O., Christoffer F<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br><br><h2>Todays work:</h2><br><b>Transformation</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br><br><h2>Results:</h2><br><b>Today:</b><br><b>Transformation:</b><br>1. pSB1C3-red: fail<br>2. pSB3C5-red: fail<br>3. pSB3T5-red: fail<br>4. pSB1A3-red: fail<br>6. pSB1K3-red: fail<br>7. pSB3K3-red: fail<br>12. pSB4K5-red: fail<br>14. pET13b-zifz:4cl-PBSII:STS: successful<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m: fail<br>17. pSB1C3-4Cl_m: fail<br>18. pSB1C3-CHS_m: fail<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF: successful<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: successful<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF: successful<br><br><h2>Other experiments:</h2><br><b>Mixing MRS-broth</b><br><b>Making of EDTA solution</b><br><b>MRS-plates preparation</b></div>'
ds = '<div id="dairy-text"><h1>Monday 2013-06-17</h1><br><b>Name of participants: </b>Kristoffer L, Emil M, Lovisa P, Theodor L, Marcus H, Ken B-A, Peter C, Christoffer A, Anton B, Nafisa B, Stephanie H, Alona N, Anders E, Malin B., Pontus D., Alexander V., Magnus B., Nils A. Thorsteinn O., Christoffer F<br><br><h2>Ongoing constructs:</h2><br><b>E. coli:</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br><br><h2>Todays work:</h2><br><b>Transformation</b><br>1. pSB1C3-red<br>2. pSB3C5-red<br>3. pSB3T5-red<br>4. pSB1A3-red<br>6. pSB1K3-red<br>7. pSB3K3-red<br>12. pSB4K5-red<br>14. pET13b-zifz:4cl-PBSII:STS<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m<br>17. pSB1C3-4Cl_m<br>18. pSB1C3-CHS_m<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br><br><h2>Results:</h2><br><b>Today:</b><br><b>Transformation:</b><br>1. pSB1C3-red: fail<br>2. pSB3C5-red: fail<br>3. pSB3T5-red: fail<br>4. pSB1A3-red: fail<br>6. pSB1K3-red: fail<br>7. pSB3K3-red: fail<br>12. pSB4K5-red: fail<br>14. pET13b-zifz:4cl-PBSII:STS: successful<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>16. pSB1C3-TAL_m: fail<br>17. pSB1C3-4Cl_m: fail<br>18. pSB1C3-CHS_m: fail<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF: successful<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: successful<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF: successful<br><br><h2>Other experiments:</h2><br><b>Mixing MRS-broth</b><br><b>Making of EDTA solution</b><br><b>MRS-plates preparation</b></div>'
 +
}
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else if(id == 'd2013613')
 +
{
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ds = '<div id="dairy-text"><h1>Thursday 2013-06-13</h1><br><b>Name of participants:</b> Alexander W, Magnus B, Malin B, Thorsteinn O, Christoffer F.<br><br><h2>Other experiments</h2><br><b>Making competent D5α</b></div>'
 +
}
 +
  else if(id == 'd2013612')
 +
{
 +
ds = '<div id="dairy-text"><h1>Wednesday 2013-06-12</h1><br><b>Name of participants: </b>Alexander W, Magnus B, Malin B, Thorsteinn O, Christoffer F., Pontus D<br><br><h2>Other experiments</h2><br><b>LB-agar plates</b></div>'
 +
}
 +
  else if(id == 'd2013610')
 +
{
 +
ds = '<div id="dairy-text"><h1>Monday 2013-06-10</h1><br><b>Name of participants: </b>Alexander W, Magnus B, Malin B, Thorsteinn O, Nils A, Christoffer F., Pontus D, Niclas S.<br><br><h2>Other experiments:</h2><br><b>LB</b><br><b>CaCl2</b><br><b>Antibiotics</b></div>'
  }
  }
  else if(id == 'd201373')
  else if(id == 'd201373')

Revision as of 01:57, 28 September 2013