Team:DTU-Denmark/Notebook/2 July 2013

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Create dilution series for biolector experiment.   
Create dilution series for biolector experiment.   
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==Who was in the lab==
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<hr/>
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Helen, Natalia, Ariadni
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==Procedure==
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<hr/>
Weighed:
Weighed:
* 2.3187g Ammonium acetate
* 2.3187g Ammonium acetate
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| Ammonium Acetate || 3
| Ammonium Acetate || 3
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==Who was in the lab==
 
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<hr/>
 
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Henrike, Jakob, Kristian, Gosia
 
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==Procedure==
 
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<hr/>
 
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[[Team:DTU-Denmark/Methods/PCR|PCR]] for 7 fragments:
 
== Conclusion from today ==
== Conclusion from today ==
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Inconsistent results from the first PCR where obtained with bands in some of the reactions but not in the duplicates. Even the g-Block was not seen though we have easily amplified that before with very bright bands. Something must have gone wrong and that's why we made the additional 34rxns which will be analyzed on gels tomorrow.
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No conclusions yet.
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Revision as of 14:52, 2 July 2013

Contents

208 lab

Main purposes today

Create dilution series for biolector experiment.


Who was in the lab

Helen, Natalia, Ariadni

Procedure

Weighed:

  • 2.3187g Ammonium acetate
  • 0.2557g Sodium nitrate
  • 0.2080g Sodium nitrite

Diluted each in 10mL M9 medium.

Created dilution series as in https://docs.google.com/spreadsheet/ccc?key=0AkM9Z7voM1otdFZ5SWstOWpURGs2S3M5bElMMllkenc

Each solution was diluted with media.

Salt mM
Sodium Nitrate 300
Sodium Nitrate 30
Sodium Nitrate 3
Sodium Nitrate 0.3
Sodium Nitrite 300 (Master)
Sodium Nitrite 30
Sodium Nitrite 3
Sodium Nitrite 0.3
Sodium Nitrite 0.03
Ammonium Acetate 3000
Ammonium Acetate 300
Ammonium Acetate 30
Ammonium Acetate 3

Conclusion from today

No conclusions yet.

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