Team:DTU-Denmark/Methods

From 2013.igem.org

(Difference between revisions)
(PCR)
(Method 2)
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[[Team:DTU-Denmark/Methods/Colony_PCR|Colony PCR]]
[[Team:DTU-Denmark/Methods/Colony_PCR|Colony PCR]]
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== Method 2 ==
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==Plasmid isolation==
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Description of method
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# Follow QIAprep Spin Miniprep Kit protocol from steps 1 - 4
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# Centrifuge for 1h at 4C and 20000 g

Revision as of 12:47, 30 July 2013

Methods

Contents


PCR

PCR

PCR-mix

PCR-ramp

PCR-touchdown

PCR-touchUp

PCR-gradient

Colony PCR

Plasmid isolation

  1. Follow QIAprep Spin Miniprep Kit protocol from steps 1 - 4
  2. Centrifuge for 1h at 4C and 20000 g