Team:Penn/MaGellinFutureDirections

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Future Directions
Future Directions

Revision as of 04:24, 22 October 2013

Penn iGEM

Future Directions


Alternative to ChIP.
  1. MaGellin can also be used to screen the binding specificity of transcription factors (TF) and other DNA binding domains, with methylation serving as an effective reporter for targeted binding.
  2. By fusing a TF to the methyltransferase, its binding with the plasmid becomes easily detected by the associated methylation
  3. In this situation, one would vary the “target site” to see which DNA sequences have the strongest binding to the TF
  4. This technique would be significantly faster, simpler, and cheaper than a ChIP-based method


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