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Team:NTU Taiwan/index.html
From 2013.igem.org
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| - | <br/><p><b>PCR</b></p><br/> | + | <br/><p><b>PCR</b></p><br/><p> |
     Step 1: Design of appropriate forward and reverse primers<br/> |      Step 1: Design of appropriate forward and reverse primers<br/> | ||
     Step 2: Prepare our template<br/> |      Step 2: Prepare our template<br/> | ||
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     Step 4: Run PCR<br/> |      Step 4: Run PCR<br/> | ||
     Step 5: Examine the results by electrophoresis<br/> |      Step 5: Examine the results by electrophoresis<br/> | ||
| - | Note: If the template is genomic DNA, we would adjust the annealing temperature at 45°C. It is because the copy number of target gene may be low. We use this annealing temp when perform PCR of Tir1, 26s, 5.8s ITS | + | Note: If the template is genomic DNA, we would adjust the annealing temperature at 45°C. It is because the copy number of target gene may be low. We use this annealing temp when perform PCR of Tir1, 26s, 5.8s ITS</p> |
| - | <br/><p><b>Construction of our parts</b></p><br/> | + | <br/><p><b>Construction of our parts</b></p><br/><p> |
     Step 1: We design primers for parts with prefix and suffix.<br/> |      Step 1: We design primers for parts with prefix and suffix.<br/> | ||
     Step 2: Perform PCR and cleanup the PCR product<br/> |      Step 2: Perform PCR and cleanup the PCR product<br/> | ||
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     Step 6: Inoculate single colony into broth with chloramphenicol.<br/> |      Step 6: Inoculate single colony into broth with chloramphenicol.<br/> | ||
     Step 7: Miniprep the plasmid DNA from the overnight broth culture.<br/> |      Step 7: Miniprep the plasmid DNA from the overnight broth culture.<br/> | ||
| - |      Step 8: Confirm the products by both RE digestion and PCR sequencing<br/> | + |      Step 8: Confirm the products by both RE digestion and PCR sequencing<br/></p> |
<p><b>Point mutation protocol</b></p> | <p><b>Point mutation protocol</b></p> | ||
Revision as of 04:17, 28 September 2013
