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Team:NTU Taiwan/index.html
From 2013.igem.org
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| - | <br/><p><b>PCR</b></p | + | <br/><p><b>PCR</b></p> |
1: Design of appropriate forward and reverse primers<br/> | 1: Design of appropriate forward and reverse primers<br/> | ||
2: Prepare our template<br/> | 2: Prepare our template<br/> | ||
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4: Run PCR<br/> | 4: Run PCR<br/> | ||
5: Examine the results by electrophoresis<br/> | 5: Examine the results by electrophoresis<br/> | ||
| - | Note: If the template is genomic DNA, we would adjust the annealing temperature at 45°C. It is because the copy number of target gene may be low. We use this annealing temp when perform PCR of Tir1, 26s, 5.8s ITS | + | Note: If the template is genomic DNA, we would adjust the annealing temperature at 45°C. It is because the copy number of target gene may be low. We use this annealing temp when perform PCR of Tir1, 26s, 5.8s ITS<br/><br/> |
| - | <br/><p><b>Construction of our parts</b></p | + | <br/><p><b>Construction of our parts</b></p><p> |
1: We design primers for parts with prefix and suffix.<br/> | 1: We design primers for parts with prefix and suffix.<br/> | ||
2: Perform PCR and cleanup the PCR product<br/> | 2: Perform PCR and cleanup the PCR product<br/> | ||
Latest revision as of 04:22, 28 September 2013
