Team:Imperial College/PHB production

From 2013.igem.org

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|[[File:Nilered.JPG|thumbnail|right|400px|<b>left</b> control cells <b>right phaCAB transformed MG1655</b>]] <br><br><br>
|[[File:Nilered.JPG|thumbnail|right|400px|<b>left</b> control cells <b>right phaCAB transformed MG1655</b>]] <br><br><br>
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O/N cutures of MG1655 transformed with either control or phaCAB plasmid were spread onto LB-agar plates with 3% glucose and Nile red staining. The staining indicates the production of P3HB.
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|[[File:27-9-13phaCABall.jpg|thumbnail|right|400px|text]]
|[[File:27-9-13phaCABall.jpg|thumbnail|right|400px|text]]
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O/N cutures of MG1655 transformed with either control or phaCAB plasmid were spread onto LB-agar plates with 3% glucose and Nile red staining. The staining indicates the production of P3HB.
<h2 class="clear">Purification of P3HB</h2>
<h2 class="clear">Purification of P3HB</h2>

Revision as of 12:15, 28 September 2013

PHB production

Nile red staining

left control cells right phaCAB transformed MG1655



text

O/N cutures of MG1655 transformed with either control or phaCAB plasmid were spread onto LB-agar plates with 3% glucose and Nile red staining. The staining indicates the production of P3HB.

Purification of P3HB

P(3HB) purified from phaCAB transformed MG1655 that were grown in LB with 3% glucose
P(3HB) purified from phaCAB transformed MG1655 that were grown in LB with 3% glucose
P(3HB) purified from phaCAB transformed MG1655 that were grown in M9M mixed waste.

Our Sponsors

TueSponsorsEppendorf.png 125px Invitrogen.jpg Geneart.jpg CSynBI.JPG

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