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| | {{:Team:DTU-Denmark/Templates/StartPage|Notebook}} | | {{:Team:DTU-Denmark/Templates/StartPage|Notebook}} |
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| - | ==Timeline==
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| - |
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| - | ===January===
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| - | * 2013-01-15 Budget completed
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| - | * 2013-01-29 Human Practices: Workshop for highschool students
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| - |
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| - | ===February===
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| - | * 2013-02-25 Project selected: Conversion of ammonia to nitrous oxide in ''E. coli''
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| - | ===March===
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| - | * 2013-03-11 Biobrick workshop hosted for KU and SDU
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| - | * 2013-03-13 Project kickoff meeting
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| - |
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| - | ===April===
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| - | * 2013-04-15 Team contract signed
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| - | * 2013-04-28 Human Practices: Outreach for highschool students
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| - |
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| - | ===May===
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| - | * 2013-05-29 "Hello World" pilot project kicked off
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| - |
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| - | ===June===
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| - | * 2013-06-04 Team competency mapping
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| - | * 2013-06-09 Work on wiki started
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| - | * 2013-06-29 Team BBQ
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| - |
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| - | ===July===
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| - | '''Week 27'''
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| - | * Hello World project is successful. From a cytoplasmic extraction, we have observed GFP in the periplasm and RFP in the cytoplasm. See a nice picture [[Team:DTU-Denmark/Notebook/6_July_2013|here.]]
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| - | * Toxicity Experiment: experiment complete, initial data analysis complete. See results at: [[Team:DTU-Denmark/ToxicityExperiment|here]]
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| - |
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| - | '''Week 28'''
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| - | * Began growing ''Nitrosomonas'' culture and made special media for it
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| - | * Designed, ordered and received primers to extract genes from ''Nitrosomonas'' and ''Pseudomonas''
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| - | * Began work on cloning genes out of ''Nitrosomonas europaea'' and ''Pseudomonas aeruginosa''. All isolations successful except Nir.
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| - |
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| - | '''Week 29'''
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| - | * Performed first nitrate -> nitrite experiment
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| - |
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| - | '''Week 30'''
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| - | * Only have colonies for the cytochromes -- other genes do not give cultures
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| - | * Attempting to extract Nir gene in two pieces, since this has not been successful so far
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| - | * Began work on arabinose inducible system
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| - |
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| - | '''Week 31'''
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| - | * Began work on Synthetic Promoter Library
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| - | * BBQ with KU this Saturday
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| - |
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| - | ===August===
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| - |
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| - | '''Week 32'''
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| - | * The week no PCR worked -- ran 96 PCRs with varying conditions to debug
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| - | * Began work on modelling
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| - | * Began work on presentation
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| - |
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| - | '''Week 33'''
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| - | * Began daily meetings to coordinate
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| - | * Arabinose inducible promoter works as of 2013-08-11
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| - | * One Nir fragment has been amplified with USER primers (changed DMSO, Mg, temperature, etc), one to go
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| - | * E coli experiment will be run again this week
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| - |
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| - | '''Week 34'''
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| - | * Decided on final human practices event
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| - |
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| - | '''Week 35'''
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| - | * Attended Scandinavian meetup in Odense hosted by SDU team
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| - | * Biobricks in progress
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| - | * Biolector for SPL in progress
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| - | * sequencing for SPL in progress
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| - | * first draft of presentation
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| - | * first draft of poster
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| - | * measure Nir, AMO, HAO transformants
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| - | * modelling of kinetics and design of a reactor: Vanessa
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| - | * Title and abstract finished; Title: Requiem for a Stream
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| - | * Reviewed presentation with Julie, who gave us very good advice on pitching our project
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| - | * measure AMO, HAO transformants complete
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| - |
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| - | ===September===
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| - | '''Week 36'''
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| - | * Have biobricks for AMO and cytochromes (are going to sequence these), the others are coming.
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| - | * Running last biolector experiment today
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| - | * sequencing for SPL is in progress
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| - | * Kristian is working on a page in the parts registry for the parts registry
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| - | * first draft of presentation completed
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| - | * first draft of poster complete
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| - | * modelling of kinetics and design of a reactor: using Continuous Stirred Tank Reactor
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| - | * Began reactor design -- ask Mikael Rordham in 223 for 2 1L chemostats
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| - |
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| - | '''Week 37'''
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| - | * biobricks -- have 2 that are fine: AMO and cytochromes
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| - | * redo colony pcr with sequencing primers to verify
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| - | * Nir experiment on friday
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| - | * Planning for bioreactor experiment
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| - | * Human Practices: Attended BioPeople event "The Basics and the Best" on IP and patent law
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| - |
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| - | '''Week 38'''
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| - | * Filmed "Bricks of Knowledge" video on USER cloning for KU team
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| - | * Biobricks mailed
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| - | ** tat signal peptide
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| - | ** amo
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| - | ** cytochromes
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| - | * arabinose promoter biobrick not made -- Kristian and Henrike will make this in the next few days
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| - | * Bioreactor experiment started this morning, will finish at 10pm tonight.
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| - |
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| - | '''Week 39'''
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| - | * Hackathon intensive to complete wiki 28th of Sept
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| - | * Nir experiment -- Kasia and Ariadne will do this on Thursday
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| - | * Video in progress
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| - | * Reviewed poster v2
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| - |
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| - | === October ===
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| - |
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| - | '''Week 40'''
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| - | * Wiki complete!
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| | ==Lab Book Entries== | | ==Lab Book Entries== |
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