Team:British Columbia/Notebook

From 2013.igem.org

(Difference between revisions)
(Team Caffeine)
(Team CRISPR)
Line 15: Line 15:
Cas9:
Cas9:
-
1) Cas9 - Joe and Anna
+
*Cas9 - Joe and Anna
-
2) pTet -  
+
*pTet -  
-
3) const. promoter + Cas9 - Joe
+
*const. promoter + Cas9 - Joe
-
4) promoter + Cas9 + Leader + R + spacer + R (on Kan)
+
*promoter + Cas9 + Leader + R + spacer + R (on Kan)
-
5) L + R + spacer + R
+
*L + R + spacer + R
Target:
Target:
-
1) tracerRNA
+
*tracerRNA
-
2) repeat + spacer + repeat - Vanins*2 + underlings
+
*repeat + spacer + repeat - Vanins*2 + underlings
-
3) leader - Ray, Fisal, Dan
+
*leader - Ray, Fisal, Dan
Decoy:
Decoy:
-
1) PAM + spacer (on Amp)  
+
*PAM + spacer (on Amp)
-
 
+
==Team Caffeine==
==Team Caffeine==

Revision as of 01:19, 4 July 2013

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions

Team CRISPR

List of BioBricks that are/will be on the way: (CAM PLEASE CONFIRM)

Cas9:

  • Cas9 - Joe and Anna
  • pTet -
  • const. promoter + Cas9 - Joe
  • promoter + Cas9 + Leader + R + spacer + R (on Kan)
  • L + R + spacer + R

Target:

  • tracerRNA
  • repeat + spacer + repeat - Vanins*2 + underlings
  • leader - Ray, Fisal, Dan

Decoy:

  • PAM + spacer (on Amp)

Team Caffeine

June 20

Joe, Dan, and I made heat-shock competent cells today - they can be found in the top compartment of the -80º Freezer #3 in the room full of freezers (thanks Cam!). We will be ordering primers (through Diane) to modify the TU Munich caffeine biosynthesis BioBricks we got in the Distribution Kit.

We're making a start - what are the other groups up to?

--grace.yi