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Team:Groningen/4 July 2013
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| - | <Br/>For the silk it is decided to try a PCR with an annealing temperature of 85 and 90 degrees Celsius. we also tried undiluted template DNA. | + | <Br/>For the silk it is decided to try a PCR with an annealing temperature of 85 and 90 degrees Celsius. we followed the usual protocol aside from the standard diluted template DNA but also tried undiluted template DNA 1, (ul in 50 ul mix for both of then) . |
<br/>1 2 3 4 5 6 | <br/>1 2 3 4 5 6 | ||
<br/>98 98 85/90 72 72 4 | <br/>98 98 85/90 72 72 4 | ||
<br/>20:00 0:30 0:25 0:27 10:00 forever | <br/>20:00 0:30 0:25 0:27 10:00 forever | ||
Revision as of 10:28, 4 July 2013
Sander and Inne""
For the silk it is decided to try a PCR with an annealing temperature of 85 and 90 degrees Celsius. we followed the usual protocol aside from the standard diluted template DNA but also tried undiluted template DNA 1, (ul in 50 ul mix for both of then) .
1 2 3 4 5 6
98 98 85/90 72 72 4
20:00 0:30 0:25 0:27 10:00 forever
