Team:NTNU-Trondheim/Press
From 2013.igem.org
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+ | <title>Berkeley iGEM 2011 </title> | ||
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+ | <meta property="og:description" | ||
+ | content="Berkeley's 2011 iGEM team wiki."/> | ||
+ | <meta name="description" content="Berkeley iGEM 2011" /> | ||
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+ | <li><a href="https://2011.igem.org/Team:Berkeley">Home </a></li> | ||
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+ | <a href="https://2011.igem.org/Team:Berkeley/Project">Project</a> | ||
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+ | <li><a href="https://2011.igem.org/Team:Berkeley/Team">Team</a></li> | ||
+ | <li><a href="https://2011.igem.org/Team:Berkeley/Judging">Judging</a></li> | ||
+ | <li><a href="https://2011.igem.org/Team:Berkeley/Notebooks">Notebooks</a></li> | ||
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+ | <img src="https://static.igem.org/mediawiki/2011/1/12/ProjectSummaryHeader.jpg" width="480"> | ||
+ | <p>Biosensors have widespread applications ranging from diagnostics to environmental monitoring. Vibrio cholerae's ToxR system can be used as a component in biological sensing devices. ToxS causes ToxR homodimerization, activating transcription of the ctx promoter. By replacing the periplasmic domain of ToxR with existing or engineered ligand-dependent homodimers, we hope to link ToxR dimerization (and gene expression) to the presence of specific ligands. Initially, ToxR constructs proved toxic to E. coli. We built a stress-regulated transcription system that drives relatively high expression of toxic proteins. This allowed us to further engineer ToxR chimeras. We fused an estrogen-dependent dimer with ToxR hoping to create an estrogen biosensor. We observed a range of constitutive phenotypes and plan more experiments to engineer a dose-dependent transcriptional response to estrogen. By fusing existing or engineered ligand dependent homodimers to ToxR, this modular system can be used to build new biosensors. </p> | ||
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+ | <div class="col3-2"style="background-color:#101a4d;"><a href="https://2011.igem.org/Team:Berkeley/Project#ToxR"> <img src="https://static.igem.org/mediawiki/igem.org/1/10/Subtitlepic1.jpg" | ||
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+ | <p style="text-align:center; color:#CECECE;"> A protein with great potential as a general biosensor system.</p> </div> | ||
+ | <div class="col3-2"style="background-color:#185f73;"><a href="https://2011.igem.org/Team:Berkeley/Project#ToxRChimera"><img src="https://static.igem.org/mediawiki/2011/b/bb/Subtitlepic3header.jpg" | ||
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+ | onmouseout="this.src='https://static.igem.org/mediawiki/2011/b/bb/Subtitlepic3header.jpg'" width="230"> </a> | ||
+ | <p style="text-align:center; color:#CECECE;"> Chimeric proteins that drive translation off of the Pctx promoter.</p></div> | ||
- | + | <div class="col3-2" style="background-color:#303285"><a href="https://2011.igem.org/Team:Berkeley/Project#StressPromoters"><img src="https://static.igem.org/mediawiki/2011/2/2b/Subtitlepic2header.jpg" | |
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+ | <p style="text-align:center; color:#CECECE;"> Our method for expressing interesting (but toxic) proteins.</p> </div> | ||
+ | |||
+ | <div class="col3-2"style="background-color:#499394"><a href="https://2011.igem.org/Team:Berkeley/Project#EstrogenBiosensor"><img src="https://static.igem.org/mediawiki/2011/c/c8/Subtitlepic4.jpg" | ||
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+ | onmouseout="this.src='https://static.igem.org/mediawiki/2011/c/c8/Subtitlepic4.jpg'" width="230"></a> | ||
+ | <p style="text-align:center; color:#CECECE;"> Bacteria engineered to detect the presence of estrogen. <br></p></div> | ||
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+ | <img src="https://static.igem.org/mediawiki/2011/b/bd/Aboutus.jpg"> | ||
+ | <p>We are Team Berkeley, a cohesive unit of 7 undergraduates and 3 advisers. Earlier this year we planned a complex and risky project, given the short amount of time iGEM made available. We quickly learned each others strengths and weaknesses and developed standard systems of organizational management in order to synchronize our efforts for the many parallel tasks at hand. We created protocols, shared them with one another, and worked together on troubleshooting. Using google docs to keep up with the status of cloning projects, the results of assays, material logistics, or the final steps required to complete a project ensured that through the months of hard work, we fine-tuned our ability to work together. As a team, we have learned firsthand how the synthetic biology community relies on the goal-oriented cooperation of skilled individuals from very different backgrounds and with very different skill sets. Some of us have strong engineering backgrounds while others of us have strong biology backgrounds, but we all share a desire to build synthetic biological systems that solve human problems. We are proud of the project that we have created which we will present at the Jamboree together in October.</p> | ||
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+ | <p style="text-align:left; color:#CECECE; font-size:13px; font-weight:bold; padding:3px"> The UC Berkeley iGEM team would like to thank Autodesk and Agilent for their financial support and Synberc, for their administrative support. </p> | ||
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Revision as of 21:01, 30 September 2013
Biosensors have widespread applications ranging from diagnostics to environmental monitoring. Vibrio cholerae's ToxR system can be used as a component in biological sensing devices. ToxS causes ToxR homodimerization, activating transcription of the ctx promoter. By replacing the periplasmic domain of ToxR with existing or engineered ligand-dependent homodimers, we hope to link ToxR dimerization (and gene expression) to the presence of specific ligands. Initially, ToxR constructs proved toxic to E. coli. We built a stress-regulated transcription system that drives relatively high expression of toxic proteins. This allowed us to further engineer ToxR chimeras. We fused an estrogen-dependent dimer with ToxR hoping to create an estrogen biosensor. We observed a range of constitutive phenotypes and plan more experiments to engineer a dose-dependent transcriptional response to estrogen. By fusing existing or engineered ligand dependent homodimers to ToxR, this modular system can be used to build new biosensors.
We are Team Berkeley, a cohesive unit of 7 undergraduates and 3 advisers. Earlier this year we planned a complex and risky project, given the short amount of time iGEM made available. We quickly learned each others strengths and weaknesses and developed standard systems of organizational management in order to synchronize our efforts for the many parallel tasks at hand. We created protocols, shared them with one another, and worked together on troubleshooting. Using google docs to keep up with the status of cloning projects, the results of assays, material logistics, or the final steps required to complete a project ensured that through the months of hard work, we fine-tuned our ability to work together. As a team, we have learned firsthand how the synthetic biology community relies on the goal-oriented cooperation of skilled individuals from very different backgrounds and with very different skill sets. Some of us have strong engineering backgrounds while others of us have strong biology backgrounds, but we all share a desire to build synthetic biological systems that solve human problems. We are proud of the project that we have created which we will present at the Jamboree together in October.
The UC Berkeley iGEM team would like to thank Autodesk and Agilent for their financial support and Synberc, for their administrative support.
- Thank you Wageningen iGEM 2013 and Berkeley iGEM 2012 for snippets of their CSS code! *
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float: left; text-align: center; color: #000050; margin: 5px;
}
- stages ul > li:before {
content: "♦"; font-size: 25px; line-height: 1px; display: block; color: #000050; background-color: #262626; border-top: 5px solid #ededed; margin: 0 0 15px 0;
}
- stages ul > li.active:before {
color: #4ee54e; text-shadow: 0px 0px 10px #56ff5f;
}
/********************************************************
- The Rest
- /
.data {
width: 80%; display: block; margin: 0 auto;
} .box {
float: right; width: 775px; background-color: rgba(17, 77, 146, 0);
} .buttons {
float: left; width: 150px; background-color: rgba(17, 77, 146, 0); margin: 3px;
} .buttons a {
text-decoration: none;
} .buttons a span {
display: block; margin-bottom: 5px;
}
- title {
margin: 0 auto; text-shadow: 2px 2px #C0C0C0; font-size: 16px;
} .names {
font-size: 20px; text-shadow: 2px 2px #C0C0C0; margin: 2px;
} .top-left {
width: 495px; background-color: rgba(17, 77, 146, 0); float: left; margin: 0px 5px 5px 0px;
} .top-right {
width: 460px; height: 389px; background-color: rgba(17, 77, 146, 0.5); display: block; float: left; margin: 0px 0px 5px 0px;
} .middle {
width: 100%; height: 300px; clear: both; background-color: rgba(17, 77, 146, 0); margin: 0px 0px 5px 0px;
} .bottom-left {
width: 315px; height: 200px; float: left; margin: 0px 5px 0px 0px;
} .bottom-middle {
width: 315px; height: 200px; float: left;
} .bottom-right {
width: 315px; height: 200px; float: left; margin: 0px 0px 0px 5px;
}
.heading {
background-color: rgba(0,44,96,1); padding: 15px 0px 15px 0px; width: 100%;
} .heading a {
color: white; font-family: 'Garamond', sans-serif; font-weight: bold; font-size: 28px; text-decoration: none; margin-left: 10px;
} .heading a:hover{
color: white; font-family: 'Garamond', sans-serif; font-weight: bold; font-size: 28px; text-decoration: none;
} .heading-large {
background-color: rgba(0,44,96,1); padding: 35px 10px 15px 15px; width: auto; margin-bottom: 10px;
} .heading-large a {
color: white; font-family: 'Garamond', sans-serif; font-weight: bold; font-size: 42px; text-decoration: none;
} .sub-heading {
background-color: rgba(0, 0, 80, 1); //background-color: rgba(65, 105, 225, 1); padding: 12px 0px 12px 12px; width: auto;
} .sub-heading a {
color: white; font-family: 'Garamond', sans-serif; font-weight: bold; font-size: 20px; text-decoration: none;
} .table, tr, td {
margin: 25px; border: 0px solid rgba(17,77,146, 0); background-color: rgba(17, 77, 146, 0); text-align: left;
} table tr, table td {
border: 0px solid rgba(17,77,146, 0);
} .button:hover {
opacity: 0.5;
}
@import url(http://fonts.googleapis.com/css?family=Capriola); /* Let's import the lovely google font, please keep this line at the top of your stylesheet */ /* Menu CSS */ /*
- cssmenu,
- main-nav ul,
- main-nav ul li,
- main-nav ul li a {
padding: 0; margin: 0; line-height: 1; font-family: 'Capriola', sans-serif;
}
- main-nav:before,
- main-nav:after,
- main-nav > ul:before,
- main-nav > ul:after {
content: ; display: table;
}
- main-nav:after,
- main-nav > ul:after {
clear: both;
}
- /
- main-nav {
zoom: 1; height: 63px; background: url() repeat-x center bottom; /* border-radius: 0px; */ /* border-style: solid; */ border-left-style: none; border-right-style: none; border-bottom-style: solid; /* border-bottom: antiquewhite; */ border-color: rgba(0,44,96,1); width: 100%; margin-top: -.46%; margin-left: 0%; z-index: 99999; position:relative;
}
- main-nav {
text-align: justify;
}
- main-nav :after {
content: ; display: inline-block; width: 100%;
}
- main-nav li {
display: inline-block;
}
- main-nav ul {
background: rgba(187,218,255,1); height: 100%; /* border: yellow 10px; */ /* border-bottom-width: 10px; */ /* border-bottom-style: solid; */ margin: 0;
}
- main-nav ul li#projectBlue {
/* width: 23%; */ }
- main-nav ul li#humanPractices {
/* width: 22%; */
}
- main-nav ul li a {
display: block; height: 37px; padding: 21px 14px 0; margin: 4px 2px 0; border-radius: 2px 2px 0 0; text-decoration: none; font-size: larger; color: rgba(0, 44, 96, 1); text-shadow: 0; font-weight: 400; opacity: .8;
}
- main-nav ul li:first-child a {
/* margin: 4px 2px 0 0; */
}
- main-nav ul li a:hover img.home-image{
background:white; }
- main-nav ul li a:hover,
- main-nav ul li.active a {
display: block; color: white; text-shadow: white; opacity: 1;
}
- main-nav ul ul {
display: none; left: 0px;
}
- main-nav ul li:hover ul{
display: block; width: 100%; height: 184px; position: absolute; margin-left: 0; z-index:10; border: rgba(0,44,96,1); border-width: 3px; border-top-style: solid; border-bottom-style: solid; /* border-left-style: solid; */ /* border-right-style: solid; */
}
- main-nav ul ul li {
width: 25%; height: 100%; left:auto; clear:none; float: left; /* margin-left: 1%; */ /* margin-right: 1%; */ /* border-color: rgba(187,218,255,1); */ /* border-width: thick; */ background: rgba(0,44,96,1); /* color: rgba(187,218,255,1); */
}
- main-nav ul ul li.odd{
/* border-style: solid; */ border-color: rgba(0,44,96,1); border-width: thick; background: rgba(187,218,255,1); /* height: 100%; */ /* border: double rgba(187,218,255,1) 37px; */ /* margin-top: 1%; */ /* margin-left: 2%; */ /* margin-right: 2%; */ width: 25%;
}
- main-nav ul li ul li a {
display: block; height: 37px; padding: 22px 14px 0; margin: 4px 2px 0; border-radius: 2px 2px 0 0; text-decoration: none; font-size: larger; color: rgba(187,218,255,1); text-shadow: 0; font-weight: 400; padding-top: inherit; margin-top: 1%; opacity: 0.9; padding-top: 7%; text-align: center;
}
- main-nav ul li ul li a :after{
display:block; text-align: start; }
- main-nav ul li ul li.odd a {
text-decoration: none; color: rgba(0, 44, 96, 1); text-shadow: 0; font-weight: 400; opacity: .9;
}
- main-nav ul li ul li.odd a:hover,
- main-nav ul li ul li.active a {
display: block; color: white; text-shadow: white; opacity: 1;
}
- main-nav ul li ul li:hover a,
- main-nav ul li ul li.active a {
display: block; /* height: 100%; */ /* margin-top: 0px; */ /* padding-top: 14%; */ color: white; text-shadow: white; opacity: 1;
}
- main-nav img.nav-image#outreach {
width: 100px; height:auto; margin-top: 11px; }
- main-nav img.nav-image#plant {
width: 180px; height:auto; margin-top: 14px; }
- main-nav img.nav-image#solubilizing {
width:100px; height:auto; }
- main-nav img.nav-image#lumi {
width: 190px; height:auto; }
- main-nav img.nav-image#safety {
width:100px; height:auto; }
- main-nav img.nav-image {
width:200px; height:85px; margin: 10%; margin-left: auto; margin-right:auto; display: block; float: middle;
}
- main-nav img.home-image {
width: 25px; height: auto; margin: 0; margin-left: auto; margin-right: 10px; margin-bottom: 0px; padding-top: -5px; display: left; float: middle; opacity: .9; background: rgba(0, 44, 96, 1);
}
- header-image {
height: auto; width: 100%; border: rgba(0,44,96,1); border-bottom-width: 0px; border-left-style: none; border-right-style: none; border-top-style: solid; border-width: 3px; margin-left: 0%; margin-top: -2%; background: white; display: block;
}
- header-image-inner {
height: 183px; width: auto; margin-top: 0%; margin: 0%; padding: 0%; /* border: double rgba(0,44,96,1); */ /* border-width: 5px; */ /* border-style: solid; */ /* outline: solid rgba(187,218,255,1); */ background: rgba(187,218,255,1); /* box-shadow: 0 0 0 2px #000, 0 0 0 3px #999, 0 0 0 9px #fa0, 0 0 0 10px #666, 0 0 0 16px #fd0, 0 0 0 18px #000; */
}
- header-image-inner-inner {
/* margin-top: 3%; */ padding-top: 1%; float: none; }
- header-image img.firstHeaderImg {
height: auto; width: 19%; margin-left: 1%;
}
- header-image img.secondHeaderImg {
height: auto; width: 50%; margin-left: 4%; margin-right: 4%;
}
- header-image img.thirdHeaderImg {
height: auto; width: 19%;
}
/* @import url(http://fonts.googleapis.com/css?family=Capriola); /* Let's import the lovely google font, please keep this line at the top of your stylesheet */ /* Menu CSS */ /*
- cssmenu1,
- cssmenu1 ul,
- cssmenu1 ul li,
- cssmenu1 ul li a {
padding: 0; margin: 0; line-height: 1; font-family: 'Capriola', sans-serif;
}
- cssmenu1:before,
- cssmenu1:after,
- cssmenu1 > ul:before,
- cssmenu1 > ul:after {
content: ; display: table;
}
- cssmenu1:after,
- cssmenu1 > ul:after {
clear: both;
}
- cssmenu1 {
zoom: 1; height: 69px; background: url(bottom-bg.png) repeat-x center bottom; border-radius: 2px; width: auto;
}
- cssmenu1 ul {
background: url(nav-bg.png) repeat-x 0px 4px; height: 69px;
}
- cssmenu1 ul li {
float: left; list-style: none;
}
- cssmenu1 ul li a {
display: block; height: 37px; padding: 22px 30px 0; margin: 4px 2px 0; border-radius: 2px 2px 0 0; text-decoration: none; font-size: 15px; color: white; text-shadow: 0 1px 1px rgba(0, 0, 0, 0.75); font-weight: 400; opacity: .9;
}
- cssmenu1 ul li:first-child a {
margin: 4px 2px 0 0;
}
- cssmenu1 ul li a:hover,
- cssmenu1 ul li.active a {
background: url(color.png) center bottom; display: block; height: 37px; margin-top: 0px; padding-top: 26px; color: #616161; text-shadow: 0 1px 1px rgba(255, 255, 255, 0.35); opacity: 1;
}