Team:Heidelberg/Templates/Del week15 AE

From 2013.igem.org

(Difference between revisions)

Revision as of 01:39, 30 September 2013

07-08-2013

Sequencing Results

We send the following samples (amplified fragments of D. acidovorans DSM-39 and backbone pSB4K5 from the partsregistry) to GATC for sequencing:

AE (FS_02-FS_03) with Primer FS_02
AE (FS_02-FS_03) with Primer FS_03

Heidelberg AE-FS 02.txt

Sequencing of DelAE(FS_02 to FS_03) with Primer FS_02

Heidelberg AE-FS 03.txt

Sequencing of DelAE(FS_02 to FS_03) with Primer FS_03

Heidelberg AlignementAE 02Sequenzierung.txt

Alignement of expected sequence of DelAE and the sequencing results with primer FS_02

Heidelberg AlignementAE 03Sequenzierung.txt

Alignement of expected sequence of DelAE and the sequencing results with primer FS_03

08-08-2013

Amplification from FS_02 to FS_03; 5.3kb

Amplifcation of DelAE and DelEF and DelOP; run at 100 V, 0.8 % gel (TAE)(07.08)

Reaction

what	µL
D. acidovorans SPH-1	1
FS_02: (1/10)	4
FS_03: (1/10)	4
Phusion flash Master Mix	10

Conditions

Biometra TProfessional Basic
Cycles	temperature [°C]	Time [s]
1	98	10
30	98	1
	58	5
	72	1:30
1	72	7min
1	12	inf

Results:

Amplification of DelAE was repeated successfully with the new strain SPH-1
bands were cut out and DNA purified using QIAquick Gel Extraction Kit
if fragment is used in Gibson assembly the amplification has to be repeated to increase the amount of product

@@ Line 6: / Line 6: @@
 <gallery>
-File:AE-FS 02.txt | Sequencing of DelAE(FS_02 to FS_03) with Primer FS_02
+File:Heidelberg_AE-FS 02.txt | Sequencing of DelAE(FS_02 to FS_03) with Primer FS_02
-File:AE-FS 03.txt | Sequencing of DelAE(FS_02 to FS_03) with Primer FS_03
+File:Heidelberg_AE-FS 03.txt | Sequencing of DelAE(FS_02 to FS_03) with Primer FS_03
-File:AlignementAE 02Sequenzierung.txt | Alignement of expected sequence of DelAE and the sequencing results with primer FS_02
+File:Heidelberg_AlignementAE 02Sequenzierung.txt | Alignement of expected sequence of DelAE and the sequencing results with primer FS_02
-File:AlignementAE 03Sequenzierung.txt | Alignement of expected sequence of DelAE and the sequencing results with primer FS_03
+File:Heidelberg_AlignementAE 03Sequenzierung.txt | Alignement of expected sequence of DelAE and the sequencing results with primer FS_03
 </gallery>
 ==08-08-2013==
 ===Amplification from FS_02 to FS_03; 5.3kb===
-[[File:20130809 DelRestFS02-FS03 FS04-FS05 FS22-FS13l.png|100px|thumb|Amplifcation of DelAE and DelEF and DelOP; run at 100 V, 0.8 % gel (TAE)(07.08)]]
+[[File:Heidelberg_20130809 DelRestFS02-FS03 FS04-FS05 FS22-FS13l.png|100px|thumb|Amplifcation of DelAE and DelEF and DelOP; run at 100 V, 0.8 % gel (TAE)(07.08)]]
-[[File:20130809 DelRestFS02-FS03 FS04-FS05 FS22-FS13l cut.png|100px|thumb|Amplifcation of DelAE and DelEF and DelOP; run at 100 V, 0.8 % gel (TAE)(07.08)]]
+[[File:Heidelberg_20130809 DelRestFS02-FS03 FS04-FS05 FS22-FS13l cut.png|100px|thumb|Amplifcation of DelAE and DelEF and DelOP; run at 100 V, 0.8 % gel (TAE)(07.08)]]
 :'''Reaction'''
 {| class="wikitable"