Team:Heidelberg/Templates/Del week12 LP

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==18-07-2013==
==18-07-2013==
===Amplification from FS_13s to FS_15s; 6.4 kb===
===Amplification from FS_13s to FS_15s; 6.4 kb===

Latest revision as of 21:45, 30 September 2013

18-07-2013

Amplification from FS_13s to FS_15s; 6.4 kb

+=with DMSO, -=without DMSO; expected amplicon sizes: LP=6.4kbp, 8-9=3.1kbp, 21-09 and 20-09=8.1kbp, 22-13s=2.6kbp, AE=5.3kbp; run at 100 V, 0.8 % gel (TAE)
Amplifications of 18.07 and 19.07, cut
Reaction of DelLP
what µl
D. acidovorans DSM-39 1
FS_13_short: (1/10) 2
FS_15_short: (1/10) 2
Phusion flash Master Mix 10
DMSO 1
dd H2O 4
Conditions of Del LP
Biorad C1000 Touch Block A
Cycles temperature [°C] Time [s]
1 98 10
12 98 1
65 ↓ 0.5 5
72 2:10
18 98 1
63 5
72 2:10
1 72 10min
1 12 inf

Results:

  • The second try to amplify DelL-P did not work. There were no bands visible on the gel.
  • It does not make sense to try this again.
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