"
Team:Heidelberg/Templates/Del week10 OP
From 2013.igem.org
(Difference between revisions)
(Created page with "==04-07-2013== === Amplification from FS_12 to FS_13; 2.7 kb=== [[File:Heidelberg_20130705 DelFG DelL DelOP.png|200px|thumb|Gel eletrophoresis of PCRs of DelF-G, DelO-P and DelL...") |
Nils.kurzawa (Talk | contribs) m |
||
| Line 1: | Line 1: | ||
| + | |||
==04-07-2013== | ==04-07-2013== | ||
=== Amplification from FS_12 to FS_13; 2.7 kb=== | === Amplification from FS_12 to FS_13; 2.7 kb=== | ||
Latest revision as of 14:07, 30 September 2013
04-07-2013
Amplification from FS_12 to FS_13; 2.7 kb
- Reaction
| what | µl |
|---|---|
| D. acidovorans DSM-39 | 1 |
| FS_12: (1/10) | 1 |
| FS_13: (1/10) | 1 |
| Phusion Master Mix | 10 |
| DMSO | 1 |
| dd H2O | 6 |
- Conditions
| Biorad MyCycler | ||
|---|---|---|
| Cycles | temperature [°C] | Time [s] |
| 1 | 98 | 10 |
| 30 | 98 | 1 |
| 72 | 40 | |
| 1 | 72 | 420 |
| 1 | 4 | inf |
Results:
- Amplification of DelOP did not work
- Amplification will be repeated to exclude that pipetting errors were the reason for the failure
