Team:Exeter/Results

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=== Red Light Module output Brick with <i>BglII</i> and <i>BamHI</i> restriction sites ===
=== Red Light Module output Brick with <i>BglII</i> and <i>BamHI</i> restriction sites ===
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[[File:1231686 710878485592782 109932996 n.jpg|left||300px|Image: 300 pixels|light to pigment repression.]]
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The two ends of GFP have been ligated together to form a complete, working protein in the successful Gibson assembly of our output bricks. GFP is constitutively expressed within DH5α cells as endogenous ENVZ phosporylates endogenous OmpR as a component of osmolarity sensing. Endogenous OmpR then binds to OmpF. Thus our output brick is working as expected. In order to test whether the output brick can be implemented into the Red light pathway and ENVZ- strain should be used to avoid false positives.
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=== Post-Freeze Work ===
=== Post-Freeze Work ===

Revision as of 10:10, 30 September 2013

Exeter iGEM 2013 · Paint by Coli

Contents

Green Light Module

We have had marked success with our Green Light Module. Not only is the magenta pigment bright and evenly synthesised, but we have also managed to show magenta synthesis is inhibited by exposure to white light. We are currently working on showing that green light specifically switches off magenta production.

Universal showing our vibrant magenta pigment

Plates grown in the dark clearly show magenta pigment

Comparison of liquid cultures grown in dark vs. light

Cyan pigment

Red Light Module output Brick with BglII and BamHI restriction sites

light to pigment repression.

The two ends of GFP have been ligated together to form a complete, working protein in the successful Gibson assembly of our output bricks. GFP is constitutively expressed within DH5α cells as endogenous ENVZ phosporylates endogenous OmpR as a component of osmolarity sensing. Endogenous OmpR then binds to OmpF. Thus our output brick is working as expected. In order to test whether the output brick can be implemented into the Red light pathway and ENVZ- strain should be used to avoid false positives.








Post-Freeze Work

Blue Light Module

Red Light Module

(Cyan working in EnvZ deficient cells)


Exeter iGEM 2013 · Paint by Coli

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