Template:Team:Bonn:NetworkData

From 2013.igem.org

(Difference between revisions)
Line 14: Line 14:
 +
 +
 +
case 1:
 +
 +
 +
content.i = 1;
 +
content.parents=[];
 +
content.childs=[2,17];
 +
content.titleShort = "Background";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
case 2:
 +
 +
 +
content.i = 2;
 +
content.parents=[];
 +
content.childs=[2,17];
 +
content.titleShort = "Proteinaktivität";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
case 3:
 +
 +
 +
content.i = 3;
 +
content.parents=[2];
 +
content.childs=[4,5];
 +
content.titleShort = "reversibel";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
case 4:
 +
 +
 +
content.i = 4;
 +
content.parents=[3];
 +
content.childs=[];
 +
content.titleShort = "Zymogen";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
case 5:
 +
 +
 +
content.i = 5;
 +
content.parents=[3];
 +
content.childs=[];
 +
content.titleShort = "Knock In";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
case 6:
 +
 +
 +
content.i = 6;
 +
content.parents=[2];
 +
content.childs=[11,10,9,7,66];
 +
content.titleShort = "reversibel";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
 +
case 7:
 +
 +
 +
content.i = 7;
 +
content.parents=[2];
 +
content.childs=[11,10,9,7];
 +
content.titleShort = "proteinabbau";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
case 8:
 +
 +
 +
content.i = 8;
 +
content.parents=[7];
 +
content.childs=[];
 +
content.titleShort = "andere abbausysteme";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
 +
case 9:
 +
 +
 +
content.i = 9;
 +
content.parents=[6];
 +
content.childs=[];
 +
content.titleShort = "Transkription";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
 +
case 10:
 +
 +
 +
content.i = 10;
 +
content.parents=[6];
 +
content.childs=[];
 +
content.titleShort = "RNAi";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
 +
case 11:
 +
 +
 +
content.i = 11;
 +
content.parents=[6];
 +
content.childs=[];
 +
content.titleShort = "direct";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
case 12:
 +
 +
 +
content.i = 12;
 +
content.parents=[7];
 +
content.childs=[13,14,15,16];
 +
content.titleShort = "Ec. ClpXP system";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
case 13:
 +
 +
 +
content.i = 13;
 +
content.parents=[12];
 +
content.childs=[];
 +
content.titleShort = "Ec. ClpXP";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
case 14:
 +
 +
 +
content.i = 13;
 +
content.parents=[12];
 +
content.childs=[];
 +
content.titleShort = "Ec. sspB";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
case 15:
 +
 +
 +
content.i = 15;
 +
content.parents=[12];
 +
content.childs=[];
 +
content.titleShort = "Ec. ssrA";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
 +
case 18:
 +
 +
 +
content.i = 18;
 +
content.parents=[17];
 +
content.childs=[19,20,21];
 +
content.titleShort = "Light";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
 +
case 19:
 +
 +
 +
content.i = 19;
 +
content.parents=[18];
 +
content.childs=[36];
 +
content.titleShort = "LOV";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
 +
case 20:
 +
 +
 +
content.i = 20;
 +
content.parents=[18];
 +
content.childs=[22,23,24,25,26,27,28,29,30,31];
 +
content.titleShort = "Other systems";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
case 21:
 +
 +
 +
content.i = 21;
 +
content.parents=[18];
 +
content.childs=[35];
 +
content.titleShort = "pDawn";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
 +
case 33:
 +
 +
 +
content.i = 33;
 +
content.parents=[17];
 +
content.childs=[];
 +
content.titleShort = "heat";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
 +
case 35:
 +
 +
 +
content.i = 35;
 +
content.parents=[21];
 +
content.childs=[37];
 +
content.titleShort = "alternative project";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
 +
case 36:
 +
 +
 +
content.i = 36;
 +
content.parents=[19];
 +
content.childs=[37];
 +
content.titleShort = "LOV-ipaA - VinD1";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
 +
case 38:
 +
 +
 +
content.i = 38;
 +
content.parents=[37];
 +
content.childs=[40,39];
 +
content.titleShort = "Outlook";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
 +
case 45:
 +
 +
 +
content.i = 45;
 +
content.parents=[37];
 +
content.childs=[46,47,48,49,50,51];
 +
content.titleShort = "Advantages";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
case 46:
 +
 +
 +
content.i = 46;
 +
content.parents=[45];
 +
content.childs=[];
 +
content.titleShort = "speed";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
 +
case 47:
 +
 +
 +
content.i = 47;
 +
content.parents=[45];
 +
content.childs=[];
 +
content.titleShort = "general applicability";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
 +
case 48:
 +
 +
 +
content.i = 48;
 +
content.parents=[45];
 +
content.childs=[];
 +
content.titleShort = "low influence";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
 +
 +
case 49:
 +
 +
 +
content.i = 49;
 +
content.parents=[45];
 +
content.childs=[];
 +
content.titleShort = "temporal control";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
 +
 +
case 50:
 +
 +
 +
content.i = 50;
 +
content.parents=[45];
 +
content.childs=[];
 +
content.titleShort = "spatial control";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
 +
 +
case 51:
 +
 +
 +
content.i = 51;
 +
content.parents=[45];
 +
content.childs=[];
 +
content.titleShort = "great activity difference";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
case 46:
 +
 +
 +
content.i = 46;
 +
content.parents=[45];
 +
content.childs=[];
 +
content.titleShort = "speed";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
 +
 +
case 53:
 +
 +
 +
content.i = 50;
 +
content.parents=[37];
 +
content.childs=[54,55,56];
 +
content.titleShort = "Applications";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
 +
 +
 +
case 54:
 +
 +
 +
content.i = 54;
 +
content.parents=[53];
 +
content.childs=[67,68];
 +
content.titleShort = "safety - Killswitch";
 +
content.titleLong = "";
 +
content.summary= "";
 +
content.text= "
 +
";
 +
content.type="Background";
 +
break;
Line 157: Line 643:
content.parents=[130];  
content.parents=[130];  
content.childs=[];  
content.childs=[];  
-
content.titleShort = "Bonn!137";  
+
content.titleShort = "Bonn";  
-
content.titleLong = "Bonn!137";  
+
content.titleLong = "Bonn";  
-
content.summary= "Description of Bonn137";  
+
content.summary= "Description of Bonn";  
content.text= "Founded by the Romans in the year 12 before Christ, birthplace of Beethoven, once Capitol of Germany- now: Bonn is a vivid place to life and  
content.text= "Founded by the Romans in the year 12 before Christ, birthplace of Beethoven, once Capitol of Germany- now: Bonn is a vivid place to life and  
study. Next to Cologne, Bonn is also set on the river Rhine. Everybody gets smitten with it´s charm- such a unique mixture of tradition and modern lifestyle.  
study. Next to Cologne, Bonn is also set on the river Rhine. Everybody gets smitten with it´s charm- such a unique mixture of tradition and modern lifestyle.  
-
Stadt. City. Ville. Bonn.!137";  
+
Stadt. City. Ville. Bonn.";  
content.type="Team";  
content.type="Team";  
break;
break;
Line 269: Line 755:
content.i = 37;
content.i = 37;
-
content.parents=[];  
+
content.parents=[12,18,36];
-
content.childs=[4];  
+
content.childs=[40,45,53,52,57,69,65];
content.titleShort = "project summary";  
content.titleShort = "project summary";  
content.titleLong = "our project in a nutshell";
content.titleLong = "our project in a nutshell";
Line 483: Line 969:
linker and (3.) a short peptide module that docks with ClpXP (SspB [XB]). <sup><a href=#163>[16.3]</a></sup> To test whether the linker length can be varied,  
linker and (3.) a short peptide module that docks with ClpXP (SspB [XB]). <sup><a href=#163>[16.3]</a></sup> To test whether the linker length can be varied,  
-
the degradation rates of GFP-DAS+4 (0.3μM) with SspB (0.15μM) were tested in vitro with 4 different linker lengths (of 5, 25, 48 and 91 amino acids). The  
+
the degradation rates of GFP-DAS+4 (0.3µM) with SspB (0.15µM) were tested in vitro with 4 different linker lengths (of 5, 25, 48 and 91 amino acids). The  
results showed that the 25 amino acids variant triggered the fastest degradation, followed by the 25 variant with 60%, 5 variant with 30% and 91 variant with  
results showed that the 25 amino acids variant triggered the fastest degradation, followed by the 25 variant with 60%, 5 variant with 30% and 91 variant with  
Line 528: Line 1,014:
constitutive promoter proB, FKBP12-SspB[XB] with the strong constitutive promoter proC and a medium-copy p15a origin of replication. For a target protein the  
constitutive promoter proB, FKBP12-SspB[XB] with the strong constitutive promoter proC and a medium-copy p15a origin of replication. For a target protein the  
-
lacI transcription repressor was used with a DAS+4-tag recombined to the C-terminus. Usually LacI represses lacZ transcription and thus production of β-
+
lacI transcription repressor was used with a DAS+4-tag recombined to the C-terminus. Usually LacI represses lacZ transcription and thus production of ß-
-
galactosidase. Therefore degradation of LacI leads to an increasing β-galactosidase activity.  As the assay showed absence of rapamycin results in no change  
+
galactosidase. Therefore degradation of LacI leads to an increasing ß-galactosidase activity.  As the assay showed absence of rapamycin results in no change  
-
of the β-galactosidase activity. Addition of rapamycin, however, leads to increasing β-galactosidase activity. Hence the system also worked in vivo (in an  
+
of the ß-galactosidase activity. Addition of rapamycin, however, leads to increasing ß-galactosidase activity. Hence the system also worked in vivo (in an  
acceptable time). <sup><a href=#161>[16.1]</a></sup> </br>
acceptable time). <sup><a href=#161>[16.1]</a></sup> </br>
Line 560: Line 1,046:
content.type="Background";
content.type="Background";
break;
break;
 +

Revision as of 08:43, 1 October 2013

Retrieved from "http://2013.igem.org/Template:Team:Bonn:NetworkData"