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Team:TU Darmstadt/protocols
From 2013.igem.org
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| - | The following page gives an overview | + | The following page gives an overview on the generalized protocols and their possible modifications that where used our 2013 iGEM project. The corresponding materials can be found in the [https://2012.igem.org/Team:TU_Darmstadt/materials materials section]. |
== In vivo == | == In vivo == | ||
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Ligation DNA Ligation] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Ligation DNA Ligation] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Quantification DNA Quantification with NanoDrop] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Quantification DNA Quantification with NanoDrop] | ||
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR PCR] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR PCR] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_DNA Purification of DNA] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_DNA Purification of DNA] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Restriction_digest Restriction digest] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Restriction_digest Restriction digest] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Salting_Out Salting Out] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Salting_Out Salting Out] | ||
| - | * [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing DNA] | + | * [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing of DNA] |
=== [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein Protein] === | === [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein Protein] === | ||
Revision as of 13:25, 1 October 2013
| Labbook | | Protocols | | Materials | |
|---|
The following page gives an overview on the generalized protocols and their possible modifications that where used our 2013 iGEM project. The corresponding materials can be found in the materials section.
Contents |
In vivo
[http://en.wikipedia.org/wiki/E._coli Escherichia coli]
- Bacterial cell culture
- Bacterial transformation
- Cell counting/plating
- Chemically competent cells
- Colony PCR
- Glycerine Stocks
- Heat Shock Transformation
- PureYield Plasmid Miniprep System
- Protein expression of LssmOrange and mKate
In vitro
Nucleic acids
[http://en.wikipedia.org/wiki/DNA DNA]
- Agarose gel electrophoresis
- Dephosphorylation
- DNA Ligation
- DNA Quantification with NanoDrop
- PCR
- Purification of DNA
- Restriction digest
- Salting Out
- [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing of DNA]
Protein
- Activity assay
- Affinity chromatography
- Immobilized metal ion affinity chromatography (IMAC)
- pNP-Assay Bacteria
- pNP-Assay Enzyme
- Proteinpurification
- Purification of Periplasmatic Proteins
- SDS-Page
