Sandelisa90 (Talk | contribs) |
Sandelisa90 (Talk | contribs) |
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<font size = "4" > Cell Surface Display </font> <BR> | <font size = "4" > Cell Surface Display </font> <BR> | ||
''Glycerol Stock'' | ''Glycerol Stock'' | ||
| - | 8hrs incoluation in LB+Chloramphenicol of a colonie from the INP_Strep-Dead transformation plate. | + | <br>8hrs incoluation at 37°C, in LB+Chloramphenicol of a colonie from the INP_Strep-Dead transformation plate. |
| + | <br>Additionnal 8hrs incubation at 37°C of the samples to make a pellet for Miniprep. | ||
| + | |||
<br><br> | <br><br> | ||
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''Innoculating Bacteris'' | ''Innoculating Bacteris'' | ||
| - | We innoculated the bacteria transformed the day before in order to do a Miniprep according to the manufacturers instructions of the qiagen Kit. | + | <br>We innoculated the bacteria transformed the day before in order to do a Miniprep according to the manufacturers instructions of the qiagen Kit. |
{{Template:EPFL2013Footer}} | {{Template:EPFL2013Footer}} | ||
Cell Surface Display
Glycerol Stock
8hrs incoluation at 37°C, in LB+Chloramphenicol of a colonie from the INP_Strep-Dead transformation plate.
Additionnal 8hrs incubation at 37°C of the samples to make a pellet for Miniprep.
Sensing
Innoculating Bacteris
We innoculated the bacteria transformed the day before in order to do a Miniprep according to the manufacturers instructions of the qiagen Kit.
"
