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Exeter/30 July 2013
From 2013.igem.org
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* Positive controls of: | * Positive controls of: | ||
| - | - RFP cut with E and S | + | - RFP cut with <i>E</i> and <i>S</i> |
| - | - RFP cut with X and P | + | - RFP cut with <i>X</i> and <i>P</i> |
| Line 55: | Line 55: | ||
We got fresh purite water each time we needed it. | We got fresh purite water each time we needed it. | ||
| - | We incubated at | + | We incubated at 37 °C for 30 minutes and at 80 °C for 20 minutes. |
Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook]. | Take me back to the [https://2013.igem.org/Team:Exeter/Notebook notebook]. | ||
Latest revision as of 21:56, 1 October 2013
Miniprep, NanoDrop, SureClean of RBS + Cph8 (K592018)
NanoDrop + Sureclean data:
| Culture | Quickstart protocol (ng/µl) | Sureclean (ng/µl) |
|---|---|---|
| RBS + Cph8 | 17.5 | 34.6 |
| B0015 | 4.3 | 16.0 |
Liquid cultures
We made liquid cultures of:
Cph8 + RBS - K592018
B0015 - Terminator
K592022 - Cyan (w/RBS and promoter)
K864404 - Promoter, RBS and cyan
K592011 - Cyan pigment
S05058
Digest
We followed advice from a PhD student and did:
- Positive controls of:
- RFP cut with E and S
- RFP cut with X and P
- Negative control of:
- Just water
The buffer and BSA were vortexed before use to make sure they were completely defrosted.
We got fresh purite water each time we needed it.
We incubated at 37 °C for 30 minutes and at 80 °C for 20 minutes.
Take me back to the notebook.
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