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Team:DTU-Denmark/Notebook/9 July 2013
From 2013.igem.org
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==Main purpose== | ==Main purpose== | ||
| - | Run gel with 7 PCR samples of Nir operon from | + | Run gel with 7 PCR samples of Nir operon from ''Pseudomonas aeruginosa'' and purified AMO, HAO and CYC from 08.07.2013. |
Purification of Nir operon and Nanodrop measurement. | Purification of Nir operon and Nanodrop measurement. | ||
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| + | Set up new PCR reaction to extract Nir from ''Pseudomonas aeruginosa''. | ||
==Who was in the lab== | ==Who was in the lab== | ||
Revision as of 17:14, 9 July 2013
Contents |
208
Main purpose
Run gel with 7 PCR samples of Nir operon from Pseudomonas aeruginosa and purified AMO, HAO and CYC from 08.07.2013.
Purification of Nir operon and Nanodrop measurement.
Set up new PCR reaction to extract Nir from Pseudomonas aeruginosa.
Who was in the lab
Ariadni,Henrike,Julia
Procedure
Run gel
Purification of Nir DNA according to the protocol QIA purification protocol. (given in the Kit)
PCR
Results
Nanodrop measurement:
- ng/ul (Nir)
- ng/ul (Nir)
- ng/ul (Nir)
