Team:UNITN-Trento/Notebook/Labposts/07/11

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(Difference between revisions)

Latest revision as of 10:34, 3 October 2013

{"date" : "2013-07-03","author" : "emil","title" : " Inocula of the plate with the product of ligation and retransformation of the ligation products ","content" : " I observed the plates but unfortunately there were some colonies also in the control (perhaps it was due to degradation of ampicillin when it was added to LB agar too hot) so I have decided to re-transform the products of ligation left following the transformation protocol.These are the results of the plate:

plate K823026+E0840 1:1

plate K823026+E0840 1:3

plate K823024+E0840 1:1

plate K823024+E0840 1:3

plate K823024+E0840 control

plate K823026+E0840 control

Moreover I did the inocula of the old plate(3 for K823026+E0840 1:1,3 for K823026+E0840 1:3,3 for K823024+E0840 1:1,3 for K823024+E0840 1:3) in 5 mL of LB with Ampicillin(1:1000).","tags" : "K832024-K823026-E0840"}

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+{"date" : "2013-07-03","author" : "emil","title" : " Inocula of the plate with the product of ligation and retransformation of the ligation products ","content" : "<html> I observed the plates but unfortunately there were some colonies also in the control (perhaps it was due to degradation of ampicillin when it was added to LB agar too hot) so I have decided to re-transform the products of ligation left following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Competent-cells-transformation\"> transformation protocol</a>.These are the results of the plate:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823026+E0840 1:1 |<html> <img src=\"\" /></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823026+E0840 1:3|<html> <img src=\"\" /></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823024+E0840 1:1|<html> <img src=\"\" /></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823024+E0840 1:3|<html> <img src=\"\" /></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823024+E0840 control|<html> <img src=\"\" /></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823026+E0840 control|<html> <img src=\"\" /></html>}}<html>Moreover I did the inocula of the old plate(3 for K823026+E0840 1:1,3 for K823026+E0840 1:3,3 for K823024+E0840 1:1,3 for K823024+E0840 1:3) in 5 mL of LB with Ampicillin(1:1000).</html>","tags" : "K832024-K823026-E0840"}
-	"date" : "2013-07-04",
-	"author" : "fabio-viola",
-	"title" : "BACILLUS SUBTILIS COMES BACK TO LIFE!",
-	"content" : "<html>  today we made glycerol stock tubes from the remaining cultures and from the inocula made yesterday, but before that we collected some bacteria to make other two liquid cultures just to have more glycerol stock tubes to put at -80°</html>",
-	"tags" : " Bacillus subtilis "
-}