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Team:UNITN-Trento/Notebook/Labposts/07/26
From 2013.igem.org
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| - | { | + | {"date" : "2013-07-10","author" : "emil","title" : " Purification of Inocula(09/7): the final countdown","content" : "<html>I (yes I'm egocentric) did the miniprep(wath a new thing!) of the inocula(I didn't purified the 1:2 due to their despicable red color) previously done(the last I hope) following(is always the same but I have to write it anyway)the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#miniprep\">miniprep protocol</a>(OOOH!that's incredible!).These are(obviously)the results of the quantification:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|results|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>Quantities</th></tr><tr><td>BBa_K823026+BBa_E0840(1:1) </td><td> 421.3ng/µl</td></tr><tr><td>BBa_K823026+BBa_E0840(1:1) </td><td> 438.6ng/µl</td></tr>tr><td>BBa_K823026+BBa_E0840(1:3) </td><td> 356.7ng/µl</td></tr><tr><td>BBa_K823026+BBa_E0840(1:3) </td><td> 415.2ng/µl</td></tr><tr><td>BBa_K823024+BBa_E0840 </td><td> 401.2ng/µl</td></tr>tr><td>BBa_K823024+BBa_E0840 </td><td> 365ng/µl</td></tr></table></center></html>}}<html>Afterwards I screened them(800 ng for each sample) following the <a href =\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Digestion\">digestion protocol</a> so I have obtained these results:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel order|<html><center><table class=\"tn-sp-table\"><tr><th>Sample</th><th>Well</th></tr> <tr><td>ladder </td><td>1</td></tr><tr><td>BBa_K823026+BBa_E0840(1:1) </td><td>2</td></tr><tr><td>BBa_K823026+BBa_E0840(1:1) </td><td>3</td></tr>tr><td>BBa_K823026+BBa_E0840(1:3) </td><td>4</td></tr><tr><td>BBa_K823026+BBa_E0840(1:3) </td><td>5</td></tr><tr><td>BBa_K823024+BBa_E0840 </td><td>6</td></tr>tr><td>BBa_K823024+BBa_E0840 </td><td>7</td></tr></table></center></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/1/15/Tn-2013_DSC0161.JPG\" width=\"450px\"></html>}}<html>As we can see in the last 4 samples the lower band is clearly at 1000 bp then the GFP is probably present, the succesfull samples were sent to be sequenced!</html>","tags" : "pSpac-PXyl-GFP"} |
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Latest revision as of 10:38, 3 October 2013
{"date" : "2013-07-10","author" : "emil","title" : " Purification of Inocula(09/7): the final countdown","content" : "I (yes I'm egocentric) did the miniprep(wath a new thing!) of the inocula(I didn't purified the 1:2 due to their despicable red color) previously done(the last I hope) following(is always the same but I have to write it anyway)the miniprep protocol(OOOH!that's incredible!).These are(obviously)the results of the quantification:
results
Afterwards I screened them(800 ng for each sample) following the digestion protocol so I have obtained these results:| Sample | Quantities |
|---|---|
| BBa_K823026+BBa_E0840(1:1) | 421.3ng/µl |
| BBa_K823026+BBa_E0840(1:1) | 438.6ng/µl | BBa_K823026+BBa_E0840(1:3) | 356.7ng/µl |
| BBa_K823026+BBa_E0840(1:3) | 415.2ng/µl |
| BBa_K823024+BBa_E0840 | 401.2ng/µl | BBa_K823024+BBa_E0840 | 365ng/µl |
gel order
As we can see in the last 4 samples the lower band is clearly at 1000 bp then the GFP is probably present, the succesfull samples were sent to be sequenced!","tags" : "pSpac-PXyl-GFP"}
| Sample | Well |
|---|---|
| ladder | 1 |
| BBa_K823026+BBa_E0840(1:1) | 2 |
| BBa_K823026+BBa_E0840(1:1) | 3 | BBa_K823026+BBa_E0840(1:3) | 4 |
| BBa_K823026+BBa_E0840(1:3) | 5 |
| BBa_K823024+BBa_E0840 | 6 | BBa_K823024+BBa_E0840 | 7 |
![](\"https://static.igem.org/mediawiki/2013/1/15/Tn-2013_DSC0161.JPG\")
