Team:UNITN-Trento/Notebook/Labposts/07/35

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(Difference between revisions)

Latest revision as of 10:40, 3 October 2013

{"date" : "2013-07-16","author" : "gabriele","title" : "A short day","content" : "I added 1µl of DpnI to G1_EX-SAMsynth-SP O/N digestion and 1µl of SAP to G2A_linear-pSB1C3 O/N digestion, and then incubated both samples at 37°C for 1.5h. Finally, I stocked both samples at -20°C because I would like to ligate them together with the short digestion that I am going to perform tomorrow.","tags" : "SAMsynthetase"}

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+{"date" : "2013-07-16","author" : "gabriele","title" : "A short day","content" : "<html>I added 1&micro;l of DpnI to G1_EX-SAMsynth-SP O/N digestion and 1&micro;l of SAP to G2A_linear-pSB1C3 O/N digestion, and then incubated both samples at 37&deg;C for 1.5h. Finally, I stocked both samples at -20&deg;C because I would like to ligate them together with the short digestion that I am going to perform tomorrow.</html>","tags" : "SAMsynthetase"}
-	"date" : "2013-07-18",
-	"author" : "fabio-bruno",
-	"title" : " blue and red light sensors!!",
-	"content" : "<html>  today we performed the miniprep protocol on our inocula. yealds are: <br>006 a: 169,3 ng/ul; <br>006 b: 204,4ng/ul; <br>016 a: 204,9 ng/ul; <br>016 b: 173,7 ng/ul; <br>030 a: 276ng/ul; <br>030 b: 375,8ng/ul; <br>082 a: 173,1ng/ul; <br>082 b: 225,9 ng/ul; <br>After that we screened 006b, 016a,082 and 030.<br></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel 1|<html><center><img src=\"https://static.igem.org/mediawiki/2013/3/3f/Tn-2013_a.jpg\" width=\"450px\" /></center></html>}}<html></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|gel 2|<html><center><img src=\"https://static.igem.org/mediawiki/2013/1/18/Tn.2013_B.jpg\" width=\"450px\" /></center></html>}}<html>As we can see from the gels, we didn’t confirm the presence of any of them!! Now we are going to do a second set of inocula!</html>",
-	"tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR"
-}