Team:UNITN-Trento/Notebook/Labposts/07/52

From 2013.igem.org

(Difference between revisions)
 
Line 1: Line 1:
-
{
+
{"date" : "2013-07-23","author" : "fabio","title" : " the blue ligation: the never ending story !","content" : "<html> this morning I added Sap to the 016 digestion and dPnl to the other two, then I purified them and quantified'em: <br>O16: 29,8 ng/ul <br>006: 16,2 ng/ul <br>R0010: 6,1 ng/ul <br>Then I started the ligation following the ligation protocol: at the end of it, I transformed 10ul of the ligation in 200 Neb10b and plated.Meanwhile Bruno miniprepped k952003 inocula and the screening was successful!!</html>","tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR- YF1_FixJ_PfixK2_amilGFP "}
-
"date" : "2013-07-24",
+
-
"author" : "gabriele",
+
-
"title" : "Inocula",
+
-
"content" : "<html>I checked the plates prepared by Caterina <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-23-caterina\">yesterday</a>.<br/><center><table class=\"tn-sp-table\"><tr><th>plate</th><th>#colonies</th><th>#inocula</th></tr><tr><td>ON Ctrl</td><td>0</td><td>0</td></tr><tr><td>ON 1:1</td><td>6</td><td>5</td></tr><tr><td>ON 1:3</td><td>0</td><td>0</td></tr><tr><td>Short Ctrl</td><td>0</td><td>0</td></tr><tr><td>Short 1:1</td><td>1</td><td>1</td></tr><tr><td>Short 1:3</td><td>1</td><td>1</td></tr></table></center><br/>As you might notice, an O/N 1:3 ligation was never performed, the problem is that Caterina wrote the wrong labels on the plates, so we can't link the plates back to the ligations... anyway, we will keep the denomination of the plates from now onward.</html>",
+
-
"tags" : "SAMsynthetase"
+
-
}
+

Latest revision as of 10:44, 3 October 2013

{"date" : "2013-07-23","author" : "fabio","title" : " the blue ligation: the never ending story !","content" : " this morning I added Sap to the 016 digestion and dPnl to the other two, then I purified them and quantified'em:
O16: 29,8 ng/ul
006: 16,2 ng/ul
R0010: 6,1 ng/ul
Then I started the ligation following the ligation protocol: at the end of it, I transformed 10ul of the ligation in 200 Neb10b and plated.Meanwhile Bruno miniprepped k952003 inocula and the screening was successful!!","tags" : " YF1_FixJ - FixK2- ho1_PcyA_cph8-OmpR- YF1_FixJ_PfixK2_amilGFP "}

Retrieved from "http://2013.igem.org/Team:UNITN-Trento/Notebook/Labposts/07/52"