From 2013.igem.org
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| - | { | + | {"date" : "2013-07- 23","author" : " emil","title" : " The incompetent Bacillus subtilis saga: The LB transformation and amplification of pSpac + GFP","content" : "<html> I have transformed 3 groups of cells with 500 ng of pSpac+pLac+RFP,I grew Bacillus in LB and when it reached OD of 1.1 I added the DNA to 400µl of cells,I let grow for 1 hour and then I plated the cell on Kanamicin plates. Afterwards I transformed Neb10β with 100 ng of K823026+E0840 following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols# Competent- cells- transformation\"> transformation protocol</ a>.</html>","tags" : " Bacillus subtilis- pSpac+GFP"} |
| - | "date" : "2013-07-24",
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| - | "author" : "thomas",
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| - | "title" : "Screening of AraC-pBAD + EFE + Venus",
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| - | "content" : "<html> This is the part five of <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-18-thomas-michele\">this experiment</a>. I proceeded with a <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#miniprep\">miniprep purification</a> of the previously prepared inocula and a <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols# Digestion\">screening digestion</a> in order to verify the construct. <br/><br/></html>{{:Team:UNITN- Trento/Templates/Styles/Spoiler|Quantification Results|<html><center><TABLE CLASS=\"tn- sp-table\"> <tr><th>Sample</ th> <th>Concentration</th></tr><tr><td>A from ligation 1:1 </td><td>852. 6 ng/ul</td></tr><tr><td>B from ligation 1:1 </td><td>1123 ng/ul</td></tr><tr><td>C from ligation 1:4 </td><td>685.8 ng/ul</td></tr><tr><td>D from ligation 1:4</td><td>2128.3 ng/ul</td></tr><tr><td>E from ligation 1:1 </td><td>1760 ng/ul</td></tr><tr><td>A from ligation 1:1 </td><td>852.6 ng/ul</td></tr></TABLE></center></html> }}<html> <br/></html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel Image|<html><center><img src=\" https://static.igem.org/mediawiki/2013/d/d9/Tn-2013_venus_screening.JPG\" style =\"width: 450px\"></center></html>}}<html> As you can see from the gel image, only one sample seems to confirm our fusion protein and will be sent for sequencing. Lane 4: insert 3024 bp, vector 2070 bp. Kapa universal ladder was adopted.</html>", | + | |
| - | "tags" : "EFE-Venus"
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| - | } | + | |
Latest revision as of 10:45, 3 October 2013
{"date" : "2013-07-23","author" : "emil","title" : "The incompetent Bacillus subtilis saga: The LB transformation and amplification of pSpac +GFP","content" : "I have transformed 3 groups of cells with 500 ng of pSpac+pLac+RFP,I grew Bacillus in LB and when it reached OD of 1.1 I added the DNA to 400µl of cells,I let grow for 1 hour and then I plated the cell on Kanamicin plates.Afterwards I transformed Neb10β with 100 ng of K823026+E0840 following the transformation protocol.","tags" : "Bacillus subtilis-pSpac+GFP"}
"
