Team:UNITN-Trento/Notebook/Labposts/07/64

From 2013.igem.org

(Difference between revisions)

Latest revision as of 10:47, 3 October 2013

{"date" : "2013-07-27","author" : "thomas","title" : "AraCpBAD + EFE + Venus transformation","content" : "Once I confirmed the construct through an electrophoresis analisis, I decided to transorm the sample C into NEB10b cells. I plated then 100 ul of cells and putted the plate in static incubation at 37°C overnight.

Plate Image

As you can see from the picture, the colonies grown. One of them was then picked up and inoculated overnight.","tags" : "EFE-Venus"}

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+{"date" : "2013-07-27","author" : "thomas","title" : "AraCpBAD + EFE + Venus transformation","content" : "<html>Once I confirmed the construct through an electrophoresis analisis, I decided to transorm the sample C into NEB10b cells. I plated then 100 ul of cells and putted the plate in static incubation at 37&deg;C overnight.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Plate Image|<html><center><img src=\"https://static.igem.org/mediawiki/2013/5/53/Tn-2013_efe%2Bvenus_plate.JPG\" style =\"width: 450px\"></center></html>}}<html> As you can see from the picture, the colonies grown. One of them was then picked up and inoculated overnight.</html>","tags" : "EFE-Venus"}
-	"date" : "2013-07-30",
-	"author" : "fabio",
-	"title" : " the blue ligation: the never ending story !",
-	"content" : "<html>  today unfortunately I miniprepped the last inocula (from ligation nr. 4) and screening didn’t verify the presence of any ligation. What a drag!! The only thing that remains to be done is to inoculate more and more colonies from previous plates: 6 from ligation 3 (plate 1:1) and 6 from ligation nr. 4 (plate 1:1).</html>",
-	"tags" : " YF1_FixJ - FixK2"
-}