Team:UNITN-Trento/Notebook/Labposts/07/09

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(Difference between revisions)

Latest revision as of 10:34, 3 October 2013

{"date" : "2013-07-03","author" : "gabriele","title" : "An easy day...","content" : "First of all I counted the colonies from yesterday:

plate	#colonies
Ctrl	4
0.5:1	2
0.5:2	0

Then, I tried again to linearize pSB1C3 (this time, starting from the circular), but I failed.

Gel

Loading scheme
1kb ladder	G1	G2	G3

$\"Gel\"$

","tags" : "SAMsynthetase-pSB1C3"}

@@ Line 1: / Line 1: @@
-{
+{"date" : "2013-07-03","author" : "gabriele","title" : "An easy day...","content" : "<html>First of all I counted the colonies from <a href=\"https://2013.igem.org/wiki/index.php?title=Team:UNITN-Trento/Notebook#tn-post-2013-07-02-gabriele\">yesterday</a>:<br/><br/><center><table class=\"tn-sp-table\"><tr><th>plate</th><th>#colonies</th></tr><tr><td>Ctrl</td><td>4</td></tr><tr><td>0.5:1</td><td>2</td></tr><tr><td>0.5:2</td><td>0</td></tr></table></center><br/><hr><br/>Then, I tried again to linearize pSB1C3 (this time, starting from the circular), but I failed.</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|Gel|<html><center><table class=\"tn-sp-table\"><tr><th colspan=\"4\">Loading scheme</th></tr><tr><td>1kb ladder</td><td>G1</td><td>G2</td><td>G3</td></tr></table><img src=\"https://static.igem.org/mediawiki/2013/d/d3/Tn-20130703-GGCM_linearizationpSB1C3_FAILED.jpg\" alt=\"Gel\" width=\"450px\"></center></html>}}<html></html>","tags" : "SAMsynthetase-pSB1C3"}
-	"date" : "2013-07-03",
-	"author" : "emil",
-	"title" : " Inocula of the plate with the product of ligation and retransformation of the ligation products ",
-	"content" : "<html> I observed the plates but unfortunately there were some colonies also in the control (perhaps it was due to degradation of ampicillin when it was added to LB agar too hot) so I have decided to re-transform the products of ligation left following the <a href=\"https://2013.igem.org/Team:UNITN-Trento/Protocols#Competent-cells-transformation\"> transformation protocol</a>.These are the results of the plate:</html>{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823026+E0840 1:1 |<html> <img src=\"\" /></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823026+E0840 1:3|<html> <img src=\"\" /></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823024+E0840 1:1|<html> <img src=\"\" /></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823024+E0840 1:3|<html> <img src=\"\" /></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823024+E0840 control|<html> <img src=\"\" /></html>}}{{:Team:UNITN-Trento/Templates/Styles/Spoiler|plate K823026+E0840 control|<html> <img src=\"\" /></html>}}<html>Moreover I did the inocula of the old plate(3 for K823026+E0840 1:1,3 for K823026+E0840 1:3,3 for K823024+E0840 1:1,3 for K823024+E0840 1:3) in 5 mL of LB with Ampicillin(1:1000).</html>",
-	"tags" : "K832024-K823026-E0840"
-}