"
Team:DTU-Denmark/Notebook/10 July 2013
From 2013.igem.org
(Difference between revisions)
(→Main purpose) |
(→Transformation in e.coli) |
||
| Line 17: | Line 17: | ||
====Transformation in ''e.coli''==== | ====Transformation in ''e.coli''==== | ||
According to the iGEM protocol ([http://parts.igem.org/Help:Protocols/Transformation transformation protocol]) with slight changes. | According to the iGEM protocol ([http://parts.igem.org/Help:Protocols/Transformation transformation protocol]) with slight changes. | ||
| - | * step 1:100 μl cells | + | * step 1: 100 μl cells |
| - | * step 2:1.5 μl of the resuspended DNA | + | * step 2: 1.5 μl of the resuspended DNA |
| - | * step 6 : 90 sec (instead of 60 s)at 42 <sup>o</sup>C | + | * step 6: 90 sec (instead of 60 s)at 42 <sup>o</sup>C |
| - | * step 9: | + | * step 9: Incubation without shaking |
| - | + | ||
| - | + | ||
| - | + | ||
==Results== | ==Results== | ||
Revision as of 11:12, 10 July 2013
Contents |
208
Main purpose
Run gel with 9 PCR samples of Nir operon from Pseudomonas aeruginosa
Transformation in E.coli
Who was in the lab
Ariadni,Henrike,Julia,Natalia
Procedure
Run gel
- 6 samples from PCR of 09.07.2013
- ladder
- 3 samples from purifcation of 09.07.2013
Transformation in e.coli
According to the iGEM protocol ([http://parts.igem.org/Help:Protocols/Transformation transformation protocol]) with slight changes.
- step 1: 100 μl cells
- step 2: 1.5 μl of the resuspended DNA
- step 6: 90 sec (instead of 60 s)at 42 oC
- step 9: Incubation without shaking
